Bone Inductive Activity of Hydroxyapatite-Bone Morphogenetic Protein Complex
Bone morphogenetic protein (BMP) is known to be a protein which induces new bone at heterotopic sites. Purification of BMP has not been perfected, and obtaining large amounis of BMP is very difficult, so it seems better to use some carrier or frame material for BMP to work effectively. Various kinds...
Saved in:
| Published in | Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Vol. 31; no. 3; pp. 860 - 869 |
|---|---|
| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
Japan
JAPANESE SOCIETY OF PERIODONTOLOGY
1989
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0385-0110 1880-408X 1880-408X |
| DOI | 10.2329/perio.31.860 |
Cover
| Abstract | Bone morphogenetic protein (BMP) is known to be a protein which induces new bone at heterotopic sites. Purification of BMP has not been perfected, and obtaining large amounis of BMP is very difficult, so it seems better to use some carrier or frame material for BMP to work effectively. Various kinds of hydroxyapatite (HAP) have been used to repair periodontal osseous defects, but they do not have osteogenetic or osteoinductive properties. If osteoinductive proteins such as BMP could retain their biologic properties after being implanted into living tissue with HAP, it would be an advantage in repairing periodontal osseous defects. In this experiment, we prepared BMP-HAP complex and investigated its osteoinductivc activity. BMP was extracted from bovine cortical bones in accordance with the Urist's procedure. The ability of this BMP to stimulate new bone growth was ensured by implantation in the muscle pouch of mice. HAP was synthesized by the wet method. The BMP-HAP complex was implanted in the muscle pouch of mice, and osteoinduction was examined 3, 7, 14, and 21 days after implantation to assess its osteo-inductive ablity. New bone formation was studied by roentgenographic and histologic observation. In the BMP-HAP group, new bone formation was seen on the roentgenograms and new cartilage and bone were observed histologically in the tissue surrounding the apatite. In the HAP group, no new cartilage or bone formation was noted. |
|---|---|
| AbstractList | Bone morphogenetic protein (BMP) is known to be a protein which induces new bone at heterotopic sites. Purification of BMP has not been perfected, and obtaining large amounts of BMP is very difficult, so it seems better to use some carrier or frame material for BMP to work effectively. Various kinds of hydroxyapatite (HAP) have been used to repair periodontal osseous defects, but they do not have osteogenetic or osteoinductive properties. If osteoinductive proteins such as BMP could retain their biologic properties after being implanted into living tissue with HAP, it would be an advantage in repairing periodontal osseous defects. In this experiment, we prepared BMP-HAP complex and investigated its osteoinductive activity. BMP was extracted from bovine cortical bones in accordance with the Urist's procedure. The ability of this BMP to stimulate new bone growth was ensured by implantation in the muscle pouch of mice. HAP was synthesized by the wet method. The BMP-HAP complex was implanted in the muscle pouch of mice, and osteoinduction was examined 3, 7, 14, and 21 days after implantation to assess its osteo-inductive ability. New bone formation was studied by roentgenographic and histologic observation. In the BMP-HAP group, new bone formation was seen on the roentgenograms and new cartilage and bone were observed histologically in the tissue surrounding the apatite. In the HAP group, no new cartilage or bone formation was noted. Bone morphogenetic protein (BMP) is known to be a protein which induces new bone at heterotopic sites. Purification of BMP has not been perfected, and obtaining large amounts of BMP is very difficult, so it seems better to use some carrier or frame material for BMP to work effectively. Various kinds of hydroxyapatite (HAP) have been used to repair periodontal osseous defects, but they do not have osteogenetic or osteoinductive properties. If osteoinductive proteins such as BMP could retain their biologic properties after being implanted into living tissue with HAP, it would be an advantage in repairing periodontal osseous defects. In this experiment, we prepared BMP-HAP complex and investigated its osteoinductive activity. BMP was extracted from bovine cortical bones in accordance with the Urist's procedure. The ability of this BMP to stimulate new bone growth was ensured by implantation in the muscle pouch of mice. HAP was synthesized by the wet method. The BMP-HAP complex was implanted in the muscle pouch of mice, and osteoinduction was examined 3, 7, 14, and 21 days after implantation to assess its osteo-inductive ability. New bone formation was studied by roentgenographic and histologic observation. In the BMP-HAP group, new bone formation was seen on the roentgenograms and new cartilage and bone were observed histologically in the tissue surrounding the apatite. In the HAP group, no new cartilage or bone formation was noted.Bone morphogenetic protein (BMP) is known to be a protein which induces new bone at heterotopic sites. Purification of BMP has not been perfected, and obtaining large amounts of BMP is very difficult, so it seems better to use some carrier or frame material for BMP to work effectively. Various kinds of hydroxyapatite (HAP) have been used to repair periodontal osseous defects, but they do not have osteogenetic or osteoinductive properties. If osteoinductive proteins such as BMP could retain their biologic properties after being implanted into living tissue with HAP, it would be an advantage in repairing periodontal osseous defects. In this experiment, we prepared BMP-HAP complex and investigated its osteoinductive activity. BMP was extracted from bovine cortical bones in accordance with the Urist's procedure. The ability of this BMP to stimulate new bone growth was ensured by implantation in the muscle pouch of mice. HAP was synthesized by the wet method. The BMP-HAP complex was implanted in the muscle pouch of mice, and osteoinduction was examined 3, 7, 14, and 21 days after implantation to assess its osteo-inductive ability. New bone formation was studied by roentgenographic and histologic observation. In the BMP-HAP group, new bone formation was seen on the roentgenograms and new cartilage and bone were observed histologically in the tissue surrounding the apatite. In the HAP group, no new cartilage or bone formation was noted. Bone morphogenetic protein (BMP) is known to be a protein which induces new bone at heterotopic sites. Purification of BMP has not been perfected, and obtaining large amounis of BMP is very difficult, so it seems better to use some carrier or frame material for BMP to work effectively. Various kinds of hydroxyapatite (HAP) have been used to repair periodontal osseous defects, but they do not have osteogenetic or osteoinductive properties. If osteoinductive proteins such as BMP could retain their biologic properties after being implanted into living tissue with HAP, it would be an advantage in repairing periodontal osseous defects. In this experiment, we prepared BMP-HAP complex and investigated its osteoinductivc activity. BMP was extracted from bovine cortical bones in accordance with the Urist's procedure. The ability of this BMP to stimulate new bone growth was ensured by implantation in the muscle pouch of mice. HAP was synthesized by the wet method. The BMP-HAP complex was implanted in the muscle pouch of mice, and osteoinduction was examined 3, 7, 14, and 21 days after implantation to assess its osteo-inductive ablity. New bone formation was studied by roentgenographic and histologic observation. In the BMP-HAP group, new bone formation was seen on the roentgenograms and new cartilage and bone were observed histologically in the tissue surrounding the apatite. In the HAP group, no new cartilage or bone formation was noted. |
| Author | KAWAI, Tatsushi KISHI, Masayuki MIEKI, Akimichi KAWAI, Tsuyoshi HASEGAWA, Jiro NOGUCHI, Toshihide UMEMURA, Masataka KATAOKA, Hiroyasu OHNO, Yuzo KURITA, Shinya KOIE, Masato |
| Author_xml | – sequence: 1 fullname: KAWAI, Tatsushi organization: Department of Dental Materials Science, School of Dentistry, Aichigakuin University – sequence: 1 fullname: KOIE, Masato organization: The 2nd Department of Oro -Maxillo-Facial Surgery, School of Dentistry, Aichigakuin University – sequence: 1 fullname: UMEMURA, Masataka organization: Department of Periodontology, School of Dentistry, Aichigakuin University – sequence: 1 fullname: MIEKI, Akimichi organization: Department of Dental Materials Science, School of Dentistry, Aichigakuin University – sequence: 1 fullname: NOGUCHI, Toshihide organization: Department of Periodontology, School of Dentistry, Aichigakuin University – sequence: 1 fullname: KURITA, Shinya organization: Department of Dental Materials Science, School of Dentistry, Aichigakuin University – sequence: 1 fullname: OHNO, Yuzo organization: Department of Periodontology, School of Dentistry, Aichigakuin University – sequence: 1 fullname: KAWAI, Tsuyoshi organization: The 2nd Department of Oro -Maxillo-Facial Surgery, School of Dentistry, Aichigakuin University – sequence: 1 fullname: KISHI, Masayuki organization: Department of Periodontology, School of Dentistry, Aichigakuin University – sequence: 1 fullname: KATAOKA, Hiroyasu organization: Department of Dental Materials Science, School of Dentistry, Aichigakuin University – sequence: 1 fullname: HASEGAWA, Jiro organization: Department of Dental Materials Science, School of Dentistry, Aichigakuin University |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/2562267$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkD1PwzAQhi0EglLYWJEyMZFytvPhjFAVqFQEA0hsluNcwCiNg50A-fekpOrKcje8z70nPcdkv7Y1EnJGYcY4y64adMbOOJ2JBPbIhAoBYQTidZ9MgIs4BErhiJx6b3IAECkDnh2SQxYnjCXphKxuhr5gWRedbs0XBtebZdo-sGVw3xfO_vSqUa1pMfwjH6xr3u0b1tgaHTw526Kpg7ldNxX-nJCDUlUeT7d7Sl5uF8_z-3D1eLecX69CzWkMoVYIMU9TgXFEi1xncRLlBS9VlnIlCqqVphmwjCcIZa5YnkDORBxFyCIaFSWfknDs7epG9d-qqmTjzFq5XlKQGy_yz4vkVA5eBv5i5BtnPzv0rVwbr7GqVI228zLN4jQdRA3g-Rbs8jUWu9atriG_HHPtrPcOy__-Lkb8w7fqDXewcoO8CkeYZonYHPBxDHe7XL8rJ7Hmv5jslm0 |
| ContentType | Journal Article |
| Copyright | The Japanese Society of Periodontology |
| Copyright_xml | – notice: The Japanese Society of Periodontology |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 ADTOC UNPAY |
| DOI | 10.2329/perio.31.860 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic Unpaywall for CDI: Periodical Content Unpaywall |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: UNPAY name: Unpaywall url: https://proxy.k.utb.cz/login?url=https://unpaywall.org/ sourceTypes: Open Access Repository |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Dentistry |
| EISSN | 1880-408X |
| EndPage | 869 |
| ExternalDocumentID | 10.2329/perio.31.860 2562267 10_2329_perio_31_860 article_perio1968_31_3_31_3_860_article_char_en |
| Genre | English Abstract Journal Article |
| GroupedDBID | ABJNI ALMA_UNASSIGNED_HOLDINGS CS3 F5P JSF KQ8 MOJWN OK1 RJT 123 2WC AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 ADTOC UNPAY |
| ID | FETCH-LOGICAL-c3150-cae053778e541dbc9564bd3fa973a8d1cac1902936e0fba2b60b28544e2414df3 |
| IEDL.DBID | UNPAY |
| ISSN | 0385-0110 1880-408X |
| IngestDate | Wed Oct 01 15:51:46 EDT 2025 Wed Oct 01 14:47:44 EDT 2025 Fri Feb 23 02:51:18 EST 2024 Tue Jul 01 01:42:33 EDT 2025 Wed Sep 03 06:30:40 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 3 |
| Language | Japanese |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c3150-cae053778e541dbc9564bd3fa973a8d1cac1902936e0fba2b60b28544e2414df3 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
| OpenAccessLink | https://proxy.k.utb.cz/login?url=https://www.jstage.jst.go.jp/article/perio1968/31/3/31_3_860/_pdf |
| PMID | 2562267 |
| PQID | 79577110 |
| PQPubID | 23479 |
| PageCount | 10 |
| ParticipantIDs | unpaywall_primary_10_2329_perio_31_860 proquest_miscellaneous_79577110 pubmed_primary_2562267 crossref_primary_10_2329_perio_31_860 jstage_primary_article_perio1968_31_3_31_3_860_article_char_en |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 1900 |
| PublicationDate | 1989-00-00 |
| PublicationDateYYYYMMDD | 1989-01-01 |
| PublicationDate_xml | – year: 1989 text: 1989-00-00 |
| PublicationDecade | 1980 |
| PublicationPlace | Japan |
| PublicationPlace_xml | – name: Japan |
| PublicationTitle | Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) |
| PublicationTitleAlternate | Nihon Shishubyo Gakkai Kaishi |
| PublicationYear | 1989 |
| Publisher | JAPANESE SOCIETY OF PERIODONTOLOGY |
| Publisher_xml | – name: JAPANESE SOCIETY OF PERIODONTOLOGY |
| References | 6) 堀田善史: ブロック状ならびに顆粒状合成ハイドロキシアパタイトのサル人工的歯周骨欠損への移植. 日歯周誌, 29: 384-404, 1987. 16) Terashima, Y. and Urist, M. R.: Brdu-inhibition of substatum-controlled chondrogenesis. Proc. soc. Exp. Biol. Med., 146: 855, 1974. 2) Nilsson, O. S., Urist. M. R., Dawson, E. G., Schmalzried, T. P. and Finerman, G. A. M.: Bone repair induced by bone morphogenetic protein in ulnar defect in dogs. Bone Joint Surg., 68-B (4) : 635-642, 1986. 8) Laemmli, U. K.: Cleavage of structual proteins during the assemble of the head of bacteriophage T 4. Nature, 227: 680-685, 1970. 3) 石川一郎, 松江美代子, 田原洋, 山田総一郎, 桐野忠昭, 松江一郎: ビーグル犬の根分岐部病変への各種移植材の比較検討. 日歯周誌, 27: 570-587.1985. 11) Urist, M. R., Huo, Y. K., Brownell, A. G., Hohl, W. M., Buyske, J., Lietze, A., Tempst, P., Hunkapiller, M. and Delange, R. J.: Purification of bovine bone morphogenetic protein by hydroxyapatite chromatography. Proc. Natl. Acad. Sci. USA., 81: 371-375, 1984. 19) 中原治彦, 高岡邦夫: 骨形成因子の臨床応用の可能性一骨誘導能を有する生体材料の開発一. 形成外科, 6: 535-542, 1987. 21) Morio Kawamura, M. D., Hisashi Iwata, M.D., Keiji Sato, M. D. and Takayuki Miura, M. D.: Chondroosteogenetic Response to Crude Bone Matrix Proteins Bound to Hydroxyapatite. Clin. Orthop., 217: 281-292, 1987. 1) Sato, K. and Urist, M. R.: Induced regeneration of calvaria by bone morphogenetic protein (BMP) in dogs. Clin. Orthop., 197: 301-311 13) Price, P. A., Urist, M. R. and Otawara, Y.: Matrix Gla-protein a new-carboxy-glutamic acid-containing protein which is associated with the organic matrix of bone. Biochem. Biophys. Res. Commun., 117: 427-475, 1983. 15) 二見 (松島) 瑞子, 稲田祐二: 高分子修復生体活性材料. 生体材料, 6: 201-207, 1988. 10) Urist, M. R. and Strates, B. S.: Bone morphogenetic protein. J. Dent. Res. (suppl. to No. 6), 50: 1392-1406, 1971. 23) 栗田新也, 河合達志, 三枝樹明道, 片岡宏康, 紀籐政司, 長谷川二郎, 梅村昌孝, 鯉江正人: HAPBMP複合体の骨形成能第2報- 骨形成能の定量一. 歯材器, 8: 87, 1989, 抄録集 4) 峯岸大造: サル歯槽骨欠損部への多孔質アパタイト顆粒の充填に関する研究. 日歯周誌, 28: 87-100, 1986. 12) Kunio Takaoka, M. D., Keiro Ono, M. D., Ph. D., Katsumasa Amitani, M. D., Ph. D., Ritsuko Kishimoto, M. S. and Yozo Nakato, M. D., Ph. D.: Solubilization and Concentration of a Bone-Inducing Substance from a Murine Osteosarcoma. Clin. Orthop., 148: 274-280, 1980. 18) 磯部饒, 荒井孝和, 富松隆, 塩野正基: 脱灰骨移植実験における間葉系細胞の分化について- マウス腹腔内diffusionchamber移植- . 骨代言射, 14: 195-199, 1981. 9) Urist, M. R.: Bone: Formation by autoindution. Science, 150: 893-899, 1965. 7) Urist, M. R., Lietze, A., Mizutani, H., Takagi, K. and Triffitt, J. T.: A bovine low molecular weight bone morphogenetic protein (BMP) fraction. Clin. Orthop., 162: 219-232, 1982. 14) John, M. Wozney, Vicki Rosen, Anthony J. Celeste, Lisa M. Mitsock, Matthew J. Whitters, Ronald W. Kriz, Rodney M. Hewick and Elizabeth A. Wang: Novel Regulators of Bone Formation: Molecular Clones and Activities. Science, 242: 1528-1534, 1988. 17) 磯部饒, 富松隆, 野田政樹, 荒井孝和, 塩野正基: 脱灰凍結乾燥骨のdiffusion chamber内移植による軟骨形成における細胞分化. 骨代謝, 13: 278-282, 1980. 5) 林成忠: ハイドロキシァパタイト顆粒の歯周治療への応用に関する組織学的研究. 日歯周誌, 28: 1004-1027, 1986. 22) Mieki, A. Kawai, T. and Hasegawa, J.: Osteoinductive Activity of 19-Tricalcium Phosphate and Bovine bone Morphogenetic Protein Complex. Third International Conference on the Chemistry and Biology of Mineralized tissues. Abstracts, 50, 1988. 20) 小川泰亮: 吸収性高分子を用いたドラッグデリバリーシステム. 生体材料, 6 (4): 209-217, 1988. |
| References_xml | – reference: 2) Nilsson, O. S., Urist. M. R., Dawson, E. G., Schmalzried, T. P. and Finerman, G. A. M.: Bone repair induced by bone morphogenetic protein in ulnar defect in dogs. Bone Joint Surg., 68-B (4) : 635-642, 1986. – reference: 5) 林成忠: ハイドロキシァパタイト顆粒の歯周治療への応用に関する組織学的研究. 日歯周誌, 28: 1004-1027, 1986. – reference: 23) 栗田新也, 河合達志, 三枝樹明道, 片岡宏康, 紀籐政司, 長谷川二郎, 梅村昌孝, 鯉江正人: HAPBMP複合体の骨形成能第2報- 骨形成能の定量一. 歯材器, 8: 87, 1989, 抄録集 – reference: 9) Urist, M. R.: Bone: Formation by autoindution. Science, 150: 893-899, 1965. – reference: 11) Urist, M. R., Huo, Y. K., Brownell, A. G., Hohl, W. M., Buyske, J., Lietze, A., Tempst, P., Hunkapiller, M. and Delange, R. J.: Purification of bovine bone morphogenetic protein by hydroxyapatite chromatography. Proc. Natl. Acad. Sci. USA., 81: 371-375, 1984. – reference: 8) Laemmli, U. K.: Cleavage of structual proteins during the assemble of the head of bacteriophage T 4. Nature, 227: 680-685, 1970. – reference: 3) 石川一郎, 松江美代子, 田原洋, 山田総一郎, 桐野忠昭, 松江一郎: ビーグル犬の根分岐部病変への各種移植材の比較検討. 日歯周誌, 27: 570-587.1985. – reference: 18) 磯部饒, 荒井孝和, 富松隆, 塩野正基: 脱灰骨移植実験における間葉系細胞の分化について- マウス腹腔内diffusionchamber移植- . 骨代言射, 14: 195-199, 1981. – reference: 4) 峯岸大造: サル歯槽骨欠損部への多孔質アパタイト顆粒の充填に関する研究. 日歯周誌, 28: 87-100, 1986. – reference: 6) 堀田善史: ブロック状ならびに顆粒状合成ハイドロキシアパタイトのサル人工的歯周骨欠損への移植. 日歯周誌, 29: 384-404, 1987. – reference: 21) Morio Kawamura, M. D., Hisashi Iwata, M.D., Keiji Sato, M. D. and Takayuki Miura, M. D.: Chondroosteogenetic Response to Crude Bone Matrix Proteins Bound to Hydroxyapatite. Clin. Orthop., 217: 281-292, 1987. – reference: 16) Terashima, Y. and Urist, M. R.: Brdu-inhibition of substatum-controlled chondrogenesis. Proc. soc. Exp. Biol. Med., 146: 855, 1974. – reference: 20) 小川泰亮: 吸収性高分子を用いたドラッグデリバリーシステム. 生体材料, 6 (4): 209-217, 1988. – reference: 1) Sato, K. and Urist, M. R.: Induced regeneration of calvaria by bone morphogenetic protein (BMP) in dogs. Clin. Orthop., 197: 301-311, – reference: 7) Urist, M. R., Lietze, A., Mizutani, H., Takagi, K. and Triffitt, J. T.: A bovine low molecular weight bone morphogenetic protein (BMP) fraction. Clin. Orthop., 162: 219-232, 1982. – reference: 10) Urist, M. R. and Strates, B. S.: Bone morphogenetic protein. J. Dent. Res. (suppl. to No. 6), 50: 1392-1406, 1971. – reference: 17) 磯部饒, 富松隆, 野田政樹, 荒井孝和, 塩野正基: 脱灰凍結乾燥骨のdiffusion chamber内移植による軟骨形成における細胞分化. 骨代謝, 13: 278-282, 1980. – reference: 12) Kunio Takaoka, M. D., Keiro Ono, M. D., Ph. D., Katsumasa Amitani, M. D., Ph. D., Ritsuko Kishimoto, M. S. and Yozo Nakato, M. D., Ph. D.: Solubilization and Concentration of a Bone-Inducing Substance from a Murine Osteosarcoma. Clin. Orthop., 148: 274-280, 1980. – reference: 22) Mieki, A. Kawai, T. and Hasegawa, J.: Osteoinductive Activity of 19-Tricalcium Phosphate and Bovine bone Morphogenetic Protein Complex. Third International Conference on the Chemistry and Biology of Mineralized tissues. Abstracts, 50, 1988. – reference: 19) 中原治彦, 高岡邦夫: 骨形成因子の臨床応用の可能性一骨誘導能を有する生体材料の開発一. 形成外科, 6: 535-542, 1987. – reference: 13) Price, P. A., Urist, M. R. and Otawara, Y.: Matrix Gla-protein a new-carboxy-glutamic acid-containing protein which is associated with the organic matrix of bone. Biochem. Biophys. Res. Commun., 117: 427-475, 1983. – reference: 14) John, M. Wozney, Vicki Rosen, Anthony J. Celeste, Lisa M. Mitsock, Matthew J. Whitters, Ronald W. Kriz, Rodney M. Hewick and Elizabeth A. Wang: Novel Regulators of Bone Formation: Molecular Clones and Activities. Science, 242: 1528-1534, 1988. – reference: 15) 二見 (松島) 瑞子, 稲田祐二: 高分子修復生体活性材料. 生体材料, 6: 201-207, 1988. |
| SSID | ssib000872039 ssib023160750 ssib002822002 ssib005901892 ssj0041828 ssib002484695 ssib005880044 ssib004298385 |
| Score | 1.2807236 |
| Snippet | Bone morphogenetic protein (BMP) is known to be a protein which induces new bone at heterotopic sites. Purification of BMP has not been perfected, and... |
| SourceID | unpaywall proquest pubmed crossref jstage |
| SourceType | Open Access Repository Aggregation Database Index Database Publisher |
| StartPage | 860 |
| SubjectTerms | Alveolar Bone Loss - therapy Animals BMP Bone and Bones Bone Morphogenetic Proteins Bone Regeneration - physiology Cattle Delivery system Durapatite Growth Substances HAP Hydroxyapatites Mice Morphogenesis Osteogenesis Osteoinduction Proteins - physiology |
| Title | Bone Inductive Activity of Hydroxyapatite-Bone Morphogenetic Protein Complex |
| URI | https://www.jstage.jst.go.jp/article/perio1968/31/3/31_3_860/_article/-char/en https://www.ncbi.nlm.nih.gov/pubmed/2562267 https://www.proquest.com/docview/79577110 https://www.jstage.jst.go.jp/article/perio1968/31/3/31_3_860/_pdf |
| UnpaywallVersion | publishedVersion |
| Volume | 31 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| ispartofPNX | Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 1989/09/28, Vol.31(3), pp.860-869 |
| journalDatabaseRights | – providerCode: PRVAFT databaseName: Open Access Digital Library customDbUrl: eissn: 1880-408X dateEnd: 99991231 omitProxy: true ssIdentifier: ssj0041828 issn: 0385-0110 databaseCode: KQ8 dateStart: 19680101 isFulltext: true titleUrlDefault: http://grweb.coalliance.org/oadl/oadl.html providerName: Colorado Alliance of Research Libraries |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1bb5VAEJ7YU5Pqg1ZtU7QXHtQ34FAuu7yY9Jqm1UYTT1KfNnujF1s4sYdo--s7wwI1pjEmvkAIG1i-Gdhv2G9mAd7iW6Z0om2gjSkCZMRxICXLAp2lqsTxg7OScoc_HecHk_TwJOvVhNedrPICedGppV14WocX06gDMaLavzW6DI-SOEpwIxLB83Ekpqacg_mc5phGMD85_rz1rZ094CRMcxUJ0E8xVuInTvyOPKJwl8OgNeRtgcr7Yemx68FDpPMpLDTVVN78lJeXvw1E-89B9Y_g9Cffw2amQn37R3XH_3rGRXjW0VR_yzV_AY9s9RIWdklaRKvDvYKP23VlfQzom_aD6VN-BC1D4delf3ZjqHOSxNozGyhqeVWjQWv0Vkqa9NvqEOeV3wra7a8lmOzvfd05CLqVGQKdIIMMtKQVJRjjNktjozQGWakySSkLlkhuYi01Eg1kErkdl0puqnysKFUztUgYUlMmyzCq8OYr4G_mSpXMpBnTVF1NFWPD8bCwBq0l49yDd71hxNQV4BAYuJABRYuVQIgQIA8-OEyHVh2iYkBUtGD2iA7nKb0NvxEebPTWFviK0byJrGzdXAtWZIyhG3mw7JxguAXyRaSvzIP3g1P8tZOv_7XhG3jixGz0r2cVRrMfjV1D9jNT6zB39IWvd15-BwY-Bvg |
| linkProvider | Unpaywall |
| linkToUnpaywall | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1bT51AEJ60xybqg7WtRqqtPLR9Aw5y2eXFRG2NadT0oSexT5u94R1O9BAvv74zLNDGNE2TvkAIG1i-Gdhv2G9mAT7gW6Z0om2gjSkCZMRxICXLAp2lqsTxg7OScoePjvODSfr1JOvVhLedrPICedGppV14WocX06gDMaLavzW6DI-SOEpwIxLB83EkpqZ8DnM5zTGNYG5y_G3nRzt7wEmY5ioSoJ9irMRPnPgdeUThLodBa8jbApW_hqUXrgd_Ip2LMN9UU_lwJ6-ufhuI9l-C6h_B6U8uw2amQv34pLrjfz3jMix1NNXfcc1fwTNbvYb5zyQtotXh3sDhbl1ZHwP6pv1g-pQfQctQ-HXpnz0Y6pwksfbMBopaXtdo0Bq9lZIm_bY6xHnlt4J2e78Ck_0v3_cOgm5lhkAnyCADLWlFCca4zdLYKI1BVqpMUsqCJZKbWEuNRAOZRG7HpZJbKh8rStVMLRKG1JTJKowqvPka-Fu5UiUzacY0VVdTxdhwPCysQWvJOPfgY28YMXUFOAQGLmRA0WIlECIEyINth-nQqkNUDIiKFswe0eE8pbfhN8KDzd7aAl8xmjeRla2bW8GKjDF0Iw9WnRMMt0C-iPSVefBpcIq_dvLtvzZchwUnZqN_PRswmt009h2yn5l63_n3T-IaBgM |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Bone+inductive+activity+of+hydroxyapatite-bone+morphogenetic+protein+complex&rft.jtitle=Nihon+Shish%C5%ABby%C5%8D+Gakkai+kaishi&rft.au=UMEMURA%2C+Masataka&rft.au=KAWAI%2C+Tatsushi&rft.au=MIEKI%2C+Akimichi&rft.au=KATAOKA%2C+Hiroyasu&rft.date=1989&rft.issn=0385-0110&rft.eissn=1880-408X&rft.volume=31&rft.issue=3&rft.spage=860&rft.epage=869&rft_id=info:doi/10.2329%2Fperio.31.860&rft.externalDBID=n%2Fa&rft.externalDocID=10_2329_perio_31_860 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0385-0110&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0385-0110&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0385-0110&client=summon |