Cytoprotective Effect of Silymarin against Diabetes-Induced Cardiomyocyte Apoptosis in Diabetic Rats

• Published in: Biomedical and environmental sciences Vol. 28; no. 1; pp. 36 - 43
• Main Authors: Tuorkey, Muobarak J., El-Desouki, Nabila I., Kamel, Rabab A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 2015; Zoology Department, Division of Physiology, Faculty of Science, Damanhour University, Damanhour 22111, Egypt%Zoology Department, Faculty of Science, Tanta University, Tanta 3152, Egypt
• Subjects: Alanine Transaminase - blood; Animals; Aspartate Aminotransferases - blood; Aspartate transaminase and Alanine transaminase ratio (AST/ALT); Bcl-2; Blood Glucose; Cardiomyocytes; Caspase-3; Cholesterol; Cholesterol - blood; Creatinine; Creatinine - blood; Diabetes Mellitus, Experimental - complications; Diabetic Cardiomyopathies - prevention & control; Heart - drug effects; Immunohistochemistry; Insulin - blood; Male; Myocardium - pathology; Myocytes, Cardiac - drug effects; Rats; Silymarin - pharmacology; Triglycerides; Triglycerides - blood; 保护作用; 心肌细胞凋亡; 水飞蓟素; 癌症治疗; 糖尿病大鼠; 胱天蛋白酶-3; Aspartate transaminase and Alanine transaminase ratio (AST/ALT); Creatinine; Bcl-2; Cardiomyocytes; Triglycerides; Caspase-3; Cholesterol
• ISSN: 0895-3988; 2214-0190
• DOI: 10.3967/bes2015.004

Objective The beneficial effects of silymarin have been extensively studied in the context of inflammation and cancer treatment, yet much less is known about its therapeutic effect on diabetes. The present study was aimed to investigate the cytoprotective activity of silymarin against diabetes-induced cardiomyocyte apoptosis. Methods Rats were randomly divided into： control group, untreated diabetes group and diabetes group treated with silymarin （120 mg/ks·d） for 10 d. Rats were sacrificed, and the cardiac muscle specimens and blood samples were collected. The immunoreactivity of caspase-3 and Bcl-2 in the cardiomyocytes was measured. Total proteins, glucose, insulin, creatinine, AST, ALT, cholesterol, and triglycerides levels were estimated. Results Unlike the treated diabetes group, cardiomyocyte apoptosis increased in the untreated rats, as evidenced by enhanced caspase-3 and declined Bcl-2 activities. The levels of glucose, creatinine, AST, ALT, cholesterol, and triglycerides declined in the treated rats. The declined levels of insulin were enhanced again after treatment of diabetic rats with silymarin, reflecting a restoration of the pancreatic β-cells activity. Conclusion The findings of this study are of great importance, which confirmed for the first time that treatment of diabetic subjects with silymarin may protect cardiomyocytes against apoptosis and promote survival-restoration of the pancreatic β-cells.