Oligodeoxynucleotides containing CpG motifs (CpG‐ODN) restores immune regulatory functions of airway macrophages of patients with asthma

• Published in: Immunology Vol. 172; no. 4; pp. 588 - 599
• Main Authors: Zhang, Huanping, Wang, Lihuan, Zhang, Aizhi, Wang, Xiangyu, Liao, Yun, Chen, Xiaoxue, Xu, Xuejie, Yang, Litao, Liu, Yu, Tang, Aifa, Yang, Pingchang
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.08.2024
• Subjects: Adult; allergy; Asthma; Asthma - immunology; Bronchoalveolar Lavage Fluid - cytology; Bronchoalveolar Lavage Fluid - immunology; Bronchus; Cells, Cultured; CpG islands; endoplasmic reticulum stress; Female; Helper cells; Humans; immune regulation; Immune system; immunity; Immunoregulation; Interleukin-10 - metabolism; Lavage; Lymphocytes T; Macrophages; Macrophages - immunology; Macrophages - metabolism; Macrophages, Alveolar - immunology; Male; Middle Aged; Oligodeoxyribonucleotides - immunology; Oligodeoxyribonucleotides - pharmacology; Oligonucleotides; Respiratory tract; Th2 Cells - immunology; allergy; asthma; immunity; endoplasmic reticulum stress; immune regulation
• ISSN: 0019-2805; 1365-2567; 1365-2567
• DOI: 10.1111/imm.13792

Allergic asthma is characterized by the polarization of Th2 cells and impaired immune regulation. Macrophages occupy the largest proportion of airway immune cells. This study aims to discover the mechanism that hinders the immune regulatory functions of airway macrophages. In this study, macrophages were isolated from cells in bronchoalveolar lavage fluids (BALF) collected from asthma patients and normal control (NC) subjects. The results indicated that macrophages occupied the largest portion of the cellular components in BALF. The frequency of IL‐10+ macrophage was significantly lower in asthma patients than in NC subjects. The expression of IL‐10 in macrophages of BALF was associated with the levels of asthma‐related parameters. The immune‐suppressive functions of BALF M0 cells were defective in asthma patients. The inducibility of IL‐10 expression was impaired in BALF macrophages of asthma patients, which could be restored by exposing to CpG. In conclusion, the induction of IL‐10 in macrophages of BALF in asthma patients was impaired, and it could be restored by exposure to CpG. When specific immune response occurs concurrently with psychological stress, endoplasmic reticulum stress was increased, which impairs the immune suppressive ability of immune regulatory T cells.