GC‐MS Analysis of Various Phytoestrogens in Health Functional Foods

This study aimed to measure phytoestrogen contents in health functional foods (HFFs) to alleviate menopausal symptoms that are commercially available in South Korea. A sensitive and selective method using gas chromatography–mass spectrometry (GC‐MS) for the monitoring of 21 phytoestrogens was develo...

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Published inBulletin of the Korean Chemical Society Vol. 38; no. 4; pp. 448 - 458
Main Authors Kim, Yu Ra, Pyo, Hee Soo, Chung, Bong Chul, Moon, Myeong Hee, Lee, Jeongae
Format Journal Article
LanguageEnglish
Published Weinheim Wiley‐VCH Verlag GmbH & Co. KGaA 01.04.2017
대한화학회
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ISSN1229-5949
0253-2964
1229-5949
DOI10.1002/bkcs.11111

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Abstract This study aimed to measure phytoestrogen contents in health functional foods (HFFs) to alleviate menopausal symptoms that are commercially available in South Korea. A sensitive and selective method using gas chromatography–mass spectrometry (GC‐MS) for the monitoring of 21 phytoestrogens was developed and fully validated. Phytoestrogens were extracted by liquid–liquid extraction using methyl tert‐butylether as the solvent. Food samples were enzymatically hydrolyzed with β‐glucuronidase/arylsulfatase. Before GC‐MS, the analytes were derivatized to their trimethylsilyl derivatives by incubation with N,O‐bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane for 30 min at 80°C. The calibration curves showed good linearity (r 2 > 0.99) in the range of interest for each analyte. The limits of detection and quantification of the 21 phytoestrogens were in the ranges of 0.5–20 and 1–50 ng/mL, respectively. The intra‐ and inter‐day precisions were all below 15.7%. These multivariate procedures were efficient in determining the optimal conditions using peak heights as responses.
AbstractList This study aimed to measure phytoestrogen contents in health functional foods (HFFs) to alleviate menopausal symptoms that are commercially available in South Korea. A sensitive and selective method using gas chromatography–mass spectrometry (GC‐MS) for the monitoring of 21 phytoestrogens was developed and fully validated. Phytoestrogens were extracted by liquid–liquid extraction using methyl tert‐butylether as the solvent. Food samples were enzymatically hydrolyzed with β‐glucuronidase/arylsulfatase. Before GC‐MS, the analytes were derivatized to their trimethylsilyl derivatives by incubation with N,O‐bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane for 30 min at 80°C. The calibration curves showed good linearity (r 2 > 0.99) in the range of interest for each analyte. The limits of detection and quantification of the 21 phytoestrogens were in the ranges of 0.5–20 and 1–50 ng/mL, respectively. The intra‐ and inter‐day precisions were all below 15.7%. These multivariate procedures were efficient in determining the optimal conditions using peak heights as responses.
This study aimed to measure phytoestrogen contents in health functional foods (HFFs) to alleviate menopausal symptoms that are commercially available in South Korea. A sensitive and selective method using gas chromatography–mass spectrometry (GC-MS) for the monitoring of 21 phytoestrogens was developed and fully validated. Phytoestrogens were extracted by liquid–liquid extraction using methyl tert-butylether as the solvent. Food samples were enzymatically hydrolyzed with β-glucuronidase/arylsulfatase. Before GC-MS, the analytes were derivatized to their trimethylsilyl derivatives by incubation with N,O-bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane for 30 min at 80°C. The calibration curves showed good linearity (r2 > 0.99) in the range of interest for each analyte. The limits of detection and quantification of the 21 phytoestrogens were in the ranges of 0.5–20 and 1–50 ng/mL, respectively. The intra- and inter-day precisions were all below 15.7%. These multivariate procedures were efficient in determining the optimal conditions using peak heights as responses. KCI Citation Count: 2
This study aimed to measure phytoestrogen contents in health functional foods ( HFFs ) to alleviate menopausal symptoms that are commercially available in South Korea. A sensitive and selective method using gas chromatography–mass spectrometry ( GC‐MS ) for the monitoring of 21 phytoestrogens was developed and fully validated. Phytoestrogens were extracted by liquid–liquid extraction using methyl tert ‐butylether as the solvent. Food samples were enzymatically hydrolyzed with β‐glucuronidase/arylsulfatase. Before GC‐MS , the analytes were derivatized to their trimethylsilyl derivatives by incubation with N , O ‐bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane for 30 min at 80°C. The calibration curves showed good linearity ( r 2  > 0.99) in the range of interest for each analyte. The limits of detection and quantification of the 21 phytoestrogens were in the ranges of 0.5–20 and 1–50 ng/ mL , respectively. The intra‐ and inter‐day precisions were all below 15.7%. These multivariate procedures were efficient in determining the optimal conditions using peak heights as responses.
Author Lee, Jeongae
Chung, Bong Chul
Kim, Yu Ra
Moon, Myeong Hee
Pyo, Hee Soo
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Snippet This study aimed to measure phytoestrogen contents in health functional foods (HFFs) to alleviate menopausal symptoms that are commercially available in South...
This study aimed to measure phytoestrogen contents in health functional foods ( HFFs ) to alleviate menopausal symptoms that are commercially available in...
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SubjectTerms Gas chromatography–mass spectrometry
Health functional food
Phytoestrogen
Simultaneous
화학
Title GC‐MS Analysis of Various Phytoestrogens in Health Functional Foods
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