GoldenBraid-compatible infectious clone of apple latent spherical virus (ALSV) and its use for virus-induced gene silencing
Virus-induced gene silencing (VIGS) is a technological process in which the expression of a plant target gene is down-regulated by inoculating a plant with a recombinant virus-based vector carrying part of the coding sequence of the target gene (Baulcombe, 1999a; Burch-Smith et al., 2004). VIGS uses...
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| Published in | Biologia plantarum Vol. 69; no. 1; pp. 38 - 48 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
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Institute of Experimental Botany of the Czech Academy of Sciences
01.01.2025
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1573-8264 0006-3134 1573-8264 |
| DOI | 10.32615/bp.2025.005 |
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| Abstract | Virus-induced gene silencing (VIGS) is a technological process in which the expression of a plant target gene is down-regulated by inoculating a plant with a recombinant virus-based vector carrying part of the coding sequence of the target gene (Baulcombe, 1999a; Burch-Smith et al., 2004). VIGS uses an RNA silencing-based defence mechanism in which double-stranded RNAs (dsRNAs) of viral origin, as templates, are processed into small interfering RNAs by Dicer-like enzymes. The resulting siRNA is incorporated into an RNA-induced silencing complex, which leads to the degradation of the RNA (viral RNA, mRNA) with sequences complementary to the siRNA. Thus, VIGS utilises foreign plant genes/targets harboured by a viral vector to produce dsRNA, a source of siRNAs that triggers RNA-mediated silencing of the corresponding target gene. VIGS has proven to be a powerful and cost-effective method for functional genomics studies in plants (Rössner et al., 2022). |
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| AbstractList | Virus-induced gene silencing (VIGS) is a technological process in which the expression of a plant target gene is down-regulated by inoculating a plant with a recombinant virus-based vector carrying part of the coding sequence of the target gene (Baulcombe, 1999a; Burch-Smith et al., 2004). VIGS uses an RNA silencing-based defence mechanism in which double-stranded RNAs (dsRNAs) of viral origin, as templates, are processed into small interfering RNAs by Dicer-like enzymes. The resulting siRNA is incorporated into an RNA-induced silencing complex, which leads to the degradation of the RNA (viral RNA, mRNA) with sequences complementary to the siRNA. Thus, VIGS utilises foreign plant genes/targets harboured by a viral vector to produce dsRNA, a source of siRNAs that triggers RNA-mediated silencing of the corresponding target gene. VIGS has proven to be a powerful and cost-effective method for functional genomics studies in plants (Rössner et al., 2022). |
| Author | MORAVEC, Tomáš DUŠEK, Jakub HOFFMEISTEROVÁ, Hana Kumar KUNDU, Jiban IBRAHIM, Emad |
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| Cites_doi | 10.1111/mpp.12529 10.1007/978-1-0716-1835-6_15 10.1371/journal.pone.0226340 10.1146/annurev-arplant-102820-020542 10.1071/CP14151 10.1186/s13007-020-0560-3 10.1016/j.virol.2004.02.008 10.1016/j.virusres.2013.06.015 10.1016/S1046-2023(03)00037-9 10.3109/13880209.2015.1048371 10.1007/s00299-014-1694-4 10.1038/s41598-018-27778-3 10.1007/s11103-010-9718-0 10.1111/tpj.16032 10.1007/s00425-010-1318-3 10.1146/annurev.pp.43.060192.002523 10.1016/j.jviromet.2006.07.017 10.1186/s13007-022-00903-0 10.1038/nprot.2006.286 10.1146/annurev-phyto-080417-050141 10.1046/j.1365-313X.1999.t01-1-00597.x 10.1016/S1369-5266(99)80022-3 10.3389/fpls.2020.522059 10.3390/v6114242 10.1128/JVI.00953-06 10.1186/1746-4811-7-15 10.1146/annurev.arplant.49.1.557 10.1111/j.1469-8137.2007.02225.x 10.1038/s41598-024-60141-3 10.3389/fmicb.2014.00595 10.1105/tpc.10.6.937 10.1094/MPMI-06-12-0161-R 10.1186/s13007-018-0286-7 10.1111/j.1467-7652.2009.00414.x 10.1006/meth.2001.1262 10.1385/1-59745-130-4:43 10.1126/science.276.5318.1558 10.1016/j.virol.2005.05.013 10.1016/j.virol.2009.01.039 10.1111/j.1365-313X.2004.02158.x 10.1385/1-59259-413-1:287 10.1007/978-3-7091-6425-9_14 10.3389/fpls.2021.699442 10.3390/ijms24065608 10.1007/s11248-010-9382-9 10.1111/j.1432-1033.1989.tb15029.x 10.1007/978-1-0716-2273-5_20 10.3389/fpls.2022.991909 10.1186/s13007-022-00950-7 10.1104/pp.111.180042 10.3390/v14020298 10.1186/s12870-021-02854-5 10.1016/j.bbrc.2003.11.177 |
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| References | Cook (ref9) 2020; 15 Inaba (ref19) 2011; 156 Li (ref24) 2000; 81 Rössner (ref38) 2022; 73 Zulfiqar (ref55) 2023; 24 Igarashi (ref18) 2009; 386 Bally (ref2) 2018; 56 Canto (ref6) 2006; 80 Kon (ref23) 2014; 5 Ratcliff (ref35) 1997; 276 Sasaki (ref41) 2011; 7 King (ref21) 2015; 34 Senthil-Kumar (ref44) 2007; 176 Dawson (ref11) 1992; 43 Romsuk (ref37) 2022; 13 Radonić (ref34) 2004; 313 Angell (ref0) 1999; 20 Chuang (ref8) 2022 Dráb (ref13) 2014; 65 Gedling (ref15) 2018; 14 Baulcombe (ref4) 1999; 2 Reis (ref36) 2018; 8 Zhou (ref54) 2022 Ma (ref29) 2021; 12 Lu (ref28) 2003; 30 Taki (ref46) 2013; 176 Satoh (ref42) 2014; 6 Zhang (ref53) 2013; 26 Moran-Bertot (ref32) 2021; 21 Cunningham (ref10) 1998; 49 Yamagishi (ref50) 2011; 75 Klińska-Bąchor (ref22) 2024; 14 Burch-Smith (ref5) 2004; 39 Zaulda (ref52) 2022; 18 Schmidt (ref43) 1989; 184 Carbonell (ref7) 2017; 18 Liu (ref25) 2020; 16 Vézina (ref48) 2009; 7 Wise (ref49) 2006 Ruiz (ref40) 1998; 10 Lombardi (ref27) 2010; 19 Baulcombe (ref3) 1999 Park (ref33) 2004; 323 Dusek (ref14) 2020; 11 Dinesh-Kumar (ref12) 2003 Annamalai (ref1) 2005; 338 Sparkes (ref45) 2006; 1 Tiedge (ref47) 2022; 18 Yamagishi (ref51) 2011; 233 Jay (ref20) 2023; 113 Mohammadzadeh (ref31) 2016; 54 Livak (ref26) 2001; 25 Mohammadzadeh (ref30) 2023; 15 Hoffmeisterová (ref16) 2022; 14 Rotenberg (ref39) 2006; 138 |
| References_xml | – volume: 18 start-page: 746 year: 2017 ident: ref7 article-title: Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection publication-title: Molecular Plant Pathology doi: 10.1111/mpp.12529 – volume: 15 start-page: 232 year: 2023 ident: ref30 article-title: Transient co-expression of bioactive murine interferon-gamma and HBsAg in tobacco and lettuce leaves publication-title: Avicenna Journal of Medical Biotechnology – volume-title: Plant Virology. Methods in Molecular Biology. Vol. 2400 year: 2022 ident: ref54 article-title: Silencing specific genes in plants using virus-induced gene silencing (VIGS) vectors doi: 10.1007/978-1-0716-1835-6_15 – volume: 15 start-page: e0226340 year: 2020 ident: ref9 article-title: Structural variation and its potential impact on genome instability: novel discoveries in the EGFR landscape by long-read sequencing publication-title: PLoS ONE doi: 10.1371/journal.pone.0226340 – volume: 73 start-page: 703 year: 2022 ident: ref38 article-title: VIGS goes viral: how VIGS transforms our understanding of plant science publication-title: Annual Review of Plant Biology doi: 10.1146/annurev-arplant-102820-020542 – volume: 65 start-page: 1323 year: 2014 ident: ref13 article-title: SYBR Green I based RT-qPCR assays for the detection of RNA viruses of cereals and grasses publication-title: Crop and Pasture Science doi: 10.1071/CP14151 – volume: 16 start-page: 9 year: 2020 ident: ref25 article-title: A cucumber green mottle mosaic virus vector for virus-induced gene silencing in cucurbit plants publication-title: Plant Methods doi: 10.1186/s13007-020-0560-3 – volume: 323 start-page: 49 year: 2004 ident: ref33 article-title: The multifunctional plant viral suppressor of gene silencing P19 interacts with itself and an RNA binding host protein publication-title: Virology doi: 10.1016/j.virol.2004.02.008 – volume: 176 start-page: 251 year: 2013 ident: ref46 article-title: Development of apple latent spherical virus-based vaccines against three tospoviruses publication-title: Virus Research doi: 10.1016/j.virusres.2013.06.015 – volume: 30 start-page: 296 year: 2003 ident: ref28 article-title: Virus-induced gene silencing in plants publication-title: Methods doi: 10.1016/S1046-2023(03)00037-9 – volume: 54 start-page: 465 year: 2016 ident: ref31 article-title: Co-expression of hepatitis C virus polytope-HBsAg and p19-silencing suppressor protein in tobacco leaves publication-title: Pharmaceutical Biology doi: 10.3109/13880209.2015.1048371 – volume: 34 start-page: 133 year: 2015 ident: ref21 article-title: Development and optimization of agroinfiltration for soybean publication-title: Plant Cell Reports doi: 10.1007/s00299-014-1694-4 – volume: 8 start-page: 9426 year: 2018 ident: ref36 article-title: A conditional silencing suppression system for transient expression publication-title: Scientific Reports doi: 10.1038/s41598-018-27778-3 – volume: 75 start-page: 193 year: 2011 ident: ref50 article-title: Promotion of flowering and reduction of a generation time in apple seedlings by ectopical expression of the Arabidopsis thaliana FT gene using the Apple latent spherical virus vector publication-title: Plant Molecular Biology doi: 10.1007/s11103-010-9718-0 – volume: 113 start-page: 186 year: 2023 ident: ref20 article-title: A contemporary reassessment of the enhanced transient expression system based on the tombusviral silencing suppressor protein P19 publication-title: The Plant Journal doi: 10.1111/tpj.16032 – volume: 233 start-page: 561 year: 2011 ident: ref51 article-title: Expression of FLOWERING LOCUS T from Arabidopsis thaliana induces precocious flowering in soybean irrespective of maturity group and stem growth habit publication-title: Planta doi: 10.1007/s00425-010-1318-3 – volume: 81 start-page: 541 year: 2000 ident: ref24 article-title: Nucleotide sequence and genome organization of Apple latent spherical virus: a new virus classified into the family Comoviridae publication-title: Journal of General Virology – volume: 43 start-page: 527 year: 1992 ident: ref11 article-title: Host-range determinants of plant viruses publication-title: Annual Review of Plant Physiology and Plant Molecular Biology doi: 10.1146/annurev.pp.43.060192.002523 – volume: 138 start-page: 49 year: 2006 ident: ref39 article-title: Methods for effective real-time RT-PCR analysis of virus-induced gene silencing publication-title: Journal of Virological Methods doi: 10.1016/j.jviromet.2006.07.017 – volume: 18 start-page: 71 year: 2022 ident: ref47 article-title: Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.) publication-title: Plant Methods doi: 10.1186/s13007-022-00903-0 – volume: 1 start-page: 2019 year: 2006 ident: ref45 article-title: Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants publication-title: Nature Protocols doi: 10.1038/nprot.2006.286 – volume: 56 start-page: 405 year: 2018 ident: ref2 article-title: The rise and rise of Nicotiana benthamiana: a plant for all reasons publication-title: Annual Review of Phytopathology doi: 10.1146/annurev-phyto-080417-050141 – volume: 20 start-page: 357 year: 1999 ident: ref0 article-title: Potato virus X amplicon-mediated silencing of nuclear genes publication-title: The Plant Journal doi: 10.1046/j.1365-313X.1999.t01-1-00597.x – volume: 2 start-page: 109 year: 1999 ident: ref4 article-title: Fast forward genetics based on virus-induced gene silencing publication-title: Current Opinion in Plant Biology doi: 10.1016/S1369-5266(99)80022-3 – volume: 11 start-page: 522059 year: 2020 ident: ref14 article-title: Extended set of GoldenBraid compatible vectors for fast assembly of multigenic constructs and their use to create geminiviral expression vectors publication-title: Frontiers in Plant Science doi: 10.3389/fpls.2020.522059 – volume: 6 start-page: 4242 year: 2014 ident: ref42 article-title: Apple latent spherical virus vector as vaccine for the prevention and treatment of mosaic diseases in pea, broad bean, and eustoma plants by Bean yellow mosaic virus publication-title: Viruses doi: 10.3390/v6114242 – volume: 80 start-page: 9064 year: 2006 ident: ref6 article-title: Translocation of Tomato bushy stunt virus P19 protein into the nucleus by ALY proteins compromises its silencing suppressor activity publication-title: Journal of Virology doi: 10.1128/JVI.00953-06 – volume: 7 start-page: 15 year: 2011 ident: ref41 article-title: Efficient virus-induced gene silencing in apple, pear and Japanese pear using Apple latent spherical virus vectors publication-title: Plant Methods doi: 10.1186/1746-4811-7-15 – volume: 49 start-page: 557 year: 1998 ident: ref10 article-title: Genes and enzymes of carotenoid biosynthesis in plants publication-title: Annual Review of Plant Physiology and Plant Molecular Biology doi: 10.1146/annurev.arplant.49.1.557 – volume: 176 start-page: 782 year: 2007 ident: ref44 article-title: A systematic study to determine the extent of gene silencing in Nicotiana benthamiana and other Solanaceae species when heterologous gene sequences are used for virus-induced gene silencing publication-title: New Phytologist doi: 10.1111/j.1469-8137.2007.02225.x – volume: 14 start-page: 9512 year: 2024 ident: ref22 article-title: Metabolic engineering of omega-3 long chain polyunsaturated fatty acids in plants using different ∆6- and ∆5-desaturases co-expressed with LPCAT from the marine diatom Phaeodactylum tricornutum publication-title: Scientific Reports doi: 10.1038/s41598-024-60141-3 – volume: 5 start-page: 595 year: 2014 ident: ref23 article-title: Induction and maintenance of DNA methylation in plant promoter sequences by apple latent spherical virus-induced transcriptional gene silencing publication-title: Frontiers in Microbiology doi: 10.3389/fmicb.2014.00595 – volume: 10 start-page: 937 year: 1998 ident: ref40 article-title: Initiation and maintenance of virus-induced gene silencing publication-title: The Plant Cell doi: 10.1105/tpc.10.6.937 – volume: 26 start-page: 182 year: 2013 ident: ref53 article-title: Optimized agroinfiltration and virus-induced gene silencing to study Ve1-mediated Verticillium resistance in tobacco publication-title: Molecular Plant-Microbe Interactions doi: 10.1094/MPMI-06-12-0161-R – volume: 14 start-page: 19 year: 2018 ident: ref15 article-title: Improved apple latent spherical virus-induced gene silencing in multiple soybean genotypes through direct inoculation of agro-infiltrated Nicotiana benthamiana extract publication-title: Plant Methods doi: 10.1186/s13007-018-0286-7 – volume: 7 start-page: 442 year: 2009 ident: ref48 article-title: Transient co-expression for fast and high-yield production of antibodies with human-like N-glycans in plants publication-title: Plant Biotechnology Journal doi: 10.1111/j.1467-7652.2009.00414.x – volume: 25 start-page: 402 year: 2001 ident: ref26 article-title: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method publication-title: Methods doi: 10.1006/meth.2001.1262 – volume-title: Agrobacterium Protocols. Methods in Molecular Biology. Vol. 343 year: 2006 ident: ref49 article-title: Three methods for the introduction of foreign DNA into Agrobacterium doi: 10.1385/1-59745-130-4:43 – volume: 276 start-page: 1558 year: 1997 ident: ref35 article-title: A similarity between viral defense and gene silencing in plants publication-title: Science doi: 10.1126/science.276.5318.1558 – volume: 338 start-page: 96 year: 2005 ident: ref1 article-title: Replication-independent expression of genome components and capsid protein of brome mosaic virus in planta: a functional role for viral replicase in RNA packaging publication-title: Virology doi: 10.1016/j.virol.2005.05.013 – volume: 386 start-page: 407 year: 2009 ident: ref18 article-title: Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes publication-title: Virology doi: 10.1016/j.virol.2009.01.039 – volume: 39 start-page: 734 year: 2004 ident: ref5 article-title: Applications and advantages of virus-induced gene silencing for gene function studies in plants publication-title: The Plant Journal doi: 10.1111/j.1365-313X.2004.02158.x – volume-title: Plant Functional Genomics. Methods in Molecular Biology. Vol. 236 year: 2003 ident: ref12 article-title: Virus-induced gene silencing doi: 10.1385/1-59259-413-1:287 – volume-title: 100 Years of Virology. Archives of Virology. Supplementa. Vol. 15 year: 1999 ident: ref3 article-title: Viruses and gene silencing in plants doi: 10.1007/978-3-7091-6425-9_14 – volume: 12 start-page: 699442 year: 2021 ident: ref29 article-title: Co-expression with replicating vector overcoming competitive effects derived by a companion protease inhibitor in plants publication-title: Frontiers in Plant Science doi: 10.3389/fpls.2021.699442 – volume: 24 start-page: 5608 year: 2023 ident: ref55 article-title: Virus-induced gene silencing (VIGS): a powerful tool for crop improvement and its advancement towards epigenetics publication-title: International Journal of Molecular Sciences doi: 10.3390/ijms24065608 – volume: 19 start-page: 1083 year: 2010 ident: ref27 article-title: Optimisation of the purification process of a tumour-targeting antibody produced in N. benthamiana using vacuum-agroinfiltration publication-title: Transgenic Research doi: 10.1007/s11248-010-9382-9 – volume: 184 start-page: 375 year: 1989 ident: ref43 article-title: Immunological detection of phytoene desaturase in algae and higher plants using an antiserum raised against a bacterial fusion-gene construct publication-title: European Journal of Biochemistry doi: 10.1111/j.1432-1033.1989.tb15029.x – volume-title: Engineering Natural Product Biosynthesis. Methods in Molecular Biology. Vol. 2489 year: 2022 ident: ref8 article-title: Rapid combinatorial coexpression of biosynthetic genes by transient expression in the plant host Nicotiana benthamiana doi: 10.1007/978-1-0716-2273-5_20 – volume: 13 start-page: 991909 year: 2022 ident: ref37 article-title: High-yield bioactive triterpenoid production by heterologous expression in Nicotiana benthamiana using the Tsukuba system publication-title: Frontiers in Plant Science doi: 10.3389/fpls.2022.991909 – volume: 18 start-page: 116 year: 2022 ident: ref52 article-title: A cowpea severe mosaic virus-based vector simplifies virus-induced gene silencing and foreign protein expression in soybean publication-title: Plant Methods doi: 10.1186/s13007-022-00950-7 – volume: 156 start-page: 2026 year: 2011 ident: ref19 article-title: Virus-induced necrosis is a consequence of direct protein-protein interaction between a viral RNA-silencing suppressor and a host catalase publication-title: Plant Physiology doi: 10.1104/pp.111.180042 – volume: 14 start-page: 298 year: 2022 ident: ref16 article-title: One-enzyme RTX-PCR for the detection of RNA viruses from multiple virus genera and crop plants publication-title: Viruses doi: 10.3390/v14020298 – volume: 21 start-page: 78 year: 2021 ident: ref32 article-title: Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae publication-title: BMC Plant Biology doi: 10.1186/s12870-021-02854-5 – volume: 313 start-page: 856 year: 2004 ident: ref34 article-title: Guideline to reference gene selection for quantitative real-time PCR publication-title: Biochemical and Biophysical Research Communications doi: 10.1016/j.bbrc.2003.11.177 |
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| SubjectTerms | agroinfiltration apple latent spherical virus goldenbraid system phytoene desaturase gene rt-qpcr virus-induced gene silencing |
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| Title | GoldenBraid-compatible infectious clone of apple latent spherical virus (ALSV) and its use for virus-induced gene silencing |
| URI | http://bp.ueb.cas.cz/doi/10.32615/bp.2025.005.pdf https://doaj.org/article/2225d992a40c44fc805325095c06ff6f |
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