Sphingobacterium jejuense sp. nov., with ginsenoside-converting activity, isolated from compost

• Published in: International journal of systematic and evolutionary microbiology Vol. 66; no. 11; pp. 4433 - 4439
• Main Authors: Siddiqi, Muhammad Zubair, Muhammad Shafi, Siddiqi, Choi, Kang Duk, Im, Wan-Taek, Aslam, Zubair
• Format: Journal Article
• Language: English
• Published: England 01.11.2016
• Subjects: Bacterial Typing Techniques; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Ginsenosides - metabolism; Panax - microbiology; Phospholipids - chemistry; Phylogeny; Republic of Korea; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Sphingobacterium - classification; Sphingobacterium - genetics; Sphingobacterium - isolation & purification; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; Republic of Korea
• ISSN: 1466-5026; 1466-5034; 1466-5034
• DOI: 10.1099/ijsem.0.001370

A Gram-stain-negative, strictly aerobic, non-motile, light yellow, short-rod bacterium (designated GJ30-7T) isolated from compost, was characterized using a polyphasic approach, in order to clarify its taxonomic position. Strain GJ30-7T was observed to grow optimally at 30 °C and at pH 7.0 on R2A agar medium. Strain Gsoil GJ30-7T possessed β-glucosidase activity, which was responsible for its ability to transform ginsenosides Rb1 and Rc (the two main active components of ginseng) to ginsenoside F2. Phylogenetic analysis, based on 16S rRNA gene sequence similarities, indicated that GJ30-7T belongs to the genus Sphingobacterium of the family Sphingobacteriaceae and was most closely related to Sphingobacterium yanglingense JCM 30166T (92.6 %), Sphingobacterium psychroaquaticum KACC 18188T (92.6 %), and Sphingobacterium thermophilum KCTC 23708T (92.0 %). The DNA G+C content was 43 mol% and MK-7 was the major isoprenoid quinone. The main polar lipids were phosphatidylethanolamine, one unidentified phospolipid and one unknown polar lipid. In contrast to standard and reference strains, unidentified sphingolipid was also present. The predominant fatty acids of strain GJ30-7T were iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 1ω7c and/or C16 : 1ω6c (summed feature 3) and iso-C17 : 1ω9c and/or C16 : 0 10-methyl (summed feature 9), supporting the affiliation of strain GJ30-7T to the genus Sphingobacterium. However, strain GJ30-7T could be distinguished genotypically and phenotypically from species of the genus Sphingobacterium with validly published names. The novel isolate therefore represents a novel species, for which the name Sphingobacterium jejuense sp. nov. is proposed, with the type strain GJ30-7T (=KACC 18625T=JCM 30948T).