Surveillance of Methicillin-Resistant Staphylococcus aureus: Cluster Analysis for Minimum Inhibitory Concentration Typing Using Spreadsheet Software
We have reported an accuracy of 73% using cluster analysis on drug sensitivity using the SPSSTM (Method S) compared to genotyping for methicillin-resistant Staphylococcus aureus (MRSA). Here we studied the efficacy of cluster analysis using ExcelTM with free additional software (Method E) on multi-d...
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Published in | Japanese Journal of Infection Prevention and Control Vol. 27; no. 5; pp. 323 - 328 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English Japanese |
Published |
Japanese Society for Infection Prevention and Control
2012
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Subjects | |
Online Access | Get full text |
ISSN | 1882-532X 1883-2407 1883-2407 |
DOI | 10.4058/jsei.27.323 |
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Abstract | We have reported an accuracy of 73% using cluster analysis on drug sensitivity using the SPSSTM (Method S) compared to genotyping for methicillin-resistant Staphylococcus aureus (MRSA). Here we studied the efficacy of cluster analysis using ExcelTM with free additional software (Method E) on multi-drug susceptibility compared to Method S. Subjects were MRSA strains first isolated in an individual. Cluster analysis was performed in (1) 71 strains using the same 11 drugs used in our previous study, (2) 70 strains using 15 drugs for the 2011 series, and (3) 70 strains using 13 drugs for the 2005 series suspected of cross infection. (1) Method E typed completely as did Method S. (2) Method E showed sensitivity, specificity, and accuracy of 97.6%, 92.9%, and 95.7% compared to Method S. (3) Method S typed four strains in one cluster separately from those of hospital personnel, indicating cross propagation rather than transmission via the staff. Hand-to-hand infection control was enforced with success. Method E showed identical typing with Method S. Methods E and S allowed typing in about three days. Therefore, typing using Method E may have advantages in analyzing MRSA cross infection, enabling rapid intervention. |
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AbstractList | We have reported an accuracy of 73% using cluster analysis on drug sensitivity using the SPSSTM (Method S) compared to genotyping for methicillin-resistant Staphylococcus aureus (MRSA). Here we studied the efficacy of cluster analysis using ExcelTM with free additional software (Method E) on multi-drug susceptibility compared to Method S. Subjects were MRSA strains first isolated in an individual. Cluster analysis was performed in (1) 71 strains using the same 11 drugs used in our previous study, (2) 70 strains using 15 drugs for the 2011 series, and (3) 70 strains using 13 drugs for the 2005 series suspected of cross infection. (1) Method E typed completely as did Method S. (2) Method E showed sensitivity, specificity, and accuracy of 97.6%, 92.9%, and 95.7% compared to Method S. (3) Method S typed four strains in one cluster separately from those of hospital personnel, indicating cross propagation rather than transmission via the staff. Hand-to-hand infection control was enforced with success. Method E showed identical typing with Method S. Methods E and S allowed typing in about three days. Therefore, typing using Method E may have advantages in analyzing MRSA cross infection, enabling rapid intervention. |
Author | ISHIMARU, Toshiyuki YAMASHITA, Akihisa ASANO, Ikuyo MATSUBARA, Nobuo UENO, Takako YOSHIDA, Junichi HIRATA, Noriko KIKUCHI, Tetsuya |
Author_xml | – sequence: 1 fullname: UENO, Takako organization: Infection Control Committee, Shimonoseki City Hospital – sequence: 1 fullname: MATSUBARA, Nobuo organization: Infection Control Committee, Shimonoseki City Hospital – sequence: 1 fullname: YAMASHITA, Akihisa organization: Department of Orthopedics, Shimonoseki City Hospital – sequence: 1 fullname: YOSHIDA, Junichi organization: Infection Control Committee, Shimonoseki City Hospital – sequence: 1 fullname: ISHIMARU, Toshiyuki organization: Infection Control Committee, Shimonoseki City Hospital – sequence: 1 fullname: KIKUCHI, Tetsuya organization: Infection Control Committee, Shimonoseki City Hospital – sequence: 1 fullname: ASANO, Ikuyo organization: Infection Control Committee, Shimonoseki City Hospital – sequence: 1 fullname: HIRATA, Noriko organization: Infection Control Committee, Shimonoseki City Hospital |
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Cites_doi | 10.1128/JCM.29.12.2690-2695.1991 10.1016/S1201-9712(01)90072-4 10.1016/S0895-4356(96)00277-6 10.1007/BF03032294 |
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References | 4) Yoshida J, Umeda A, Ishimaru T, Akao M: Cluster analysis on multiple drugs susceptibility supplements genotyping of methicillin-resistant Staphylococcus aureus. Int J Infect Dis 2001; 5: 205–8. 6) 佐藤正一,齊藤佳子,佐藤洋子,澤田恭子:MRSAにおける薬剤感受性クラスター解析およびパルスフィールド・ゲル電気泳動パターンの関連性.医学検査 2008; 57(3): 229–35. 7) 松原伸夫:ICU・手術室でのスリッパ履き替えの評価.山口臨技 2002; 26(3): 4–10. 2) Yoshida J, Nagata T, Nishioka Y, Nose Y, Tanaka M: Outbreak of multi-drug resistant Staphylococcus aureus: a cluster analysis. J Clin Epidemiol 1996; 49: 1447–52. 1) Harris AD, Furuno JP, Roghmann MC, Johnson JK, Conway LJ, Venezia RA, et al.: Targeted surveillance of methicillin-resistant Staphylococcus aureus and its potential use to guide empiric antibiotic therapy. Antimicrob Agents Chemother 2010; 54: 3143–8. 5) Ichiyama S, Ohta M, Shimokata K, Kato N, Takeuchi J: Genomic DNA fingerprinting by pulsed-field gel electrophoresis as an epidemiological marker for study of nosocomial infections caused by methicillin-resistant Staphylococcus aureus. J Clin Microbiol 1991; 29: 2690–5. 8) Yoshida J, Kirikae T, Yamanaka N, Suzuki H, Onzuka T, Hisahara M, et al.: Evidence-based infection control in thoracic surgery. Jpn J Thorac Cardiovasc Surg 2002; 50(7): 273–9. 3) Yoshida J, Ishimaru T, Kawano S, Akao M: An electronic system combining MIC2000 (TM) and antibiogram cluster analysis for surveillance of methicillin-resistant Staphylococcus aureus in hospitals. Jpn J Infect Dis 2000; 53: 79–81. 1 2 3 4 5 6 |
References_xml | – reference: 1) Harris AD, Furuno JP, Roghmann MC, Johnson JK, Conway LJ, Venezia RA, et al.: Targeted surveillance of methicillin-resistant Staphylococcus aureus and its potential use to guide empiric antibiotic therapy. Antimicrob Agents Chemother 2010; 54: 3143–8. – reference: 8) Yoshida J, Kirikae T, Yamanaka N, Suzuki H, Onzuka T, Hisahara M, et al.: Evidence-based infection control in thoracic surgery. Jpn J Thorac Cardiovasc Surg 2002; 50(7): 273–9. – reference: 7) 松原伸夫:ICU・手術室でのスリッパ履き替えの評価.山口臨技 2002; 26(3): 4–10. – reference: 3) Yoshida J, Ishimaru T, Kawano S, Akao M: An electronic system combining MIC2000 (TM) and antibiogram cluster analysis for surveillance of methicillin-resistant Staphylococcus aureus in hospitals. Jpn J Infect Dis 2000; 53: 79–81. – reference: 5) Ichiyama S, Ohta M, Shimokata K, Kato N, Takeuchi J: Genomic DNA fingerprinting by pulsed-field gel electrophoresis as an epidemiological marker for study of nosocomial infections caused by methicillin-resistant Staphylococcus aureus. J Clin Microbiol 1991; 29: 2690–5. – reference: 6) 佐藤正一,齊藤佳子,佐藤洋子,澤田恭子:MRSAにおける薬剤感受性クラスター解析およびパルスフィールド・ゲル電気泳動パターンの関連性.医学検査 2008; 57(3): 229–35. – reference: 2) Yoshida J, Nagata T, Nishioka Y, Nose Y, Tanaka M: Outbreak of multi-drug resistant Staphylococcus aureus: a cluster analysis. J Clin Epidemiol 1996; 49: 1447–52. – reference: 4) Yoshida J, Umeda A, Ishimaru T, Akao M: Cluster analysis on multiple drugs susceptibility supplements genotyping of methicillin-resistant Staphylococcus aureus. Int J Infect Dis 2001; 5: 205–8. – ident: 3 doi: 10.1128/JCM.29.12.2690-2695.1991 – ident: 2 doi: 10.1016/S1201-9712(01)90072-4 – ident: 5 – ident: 4 – ident: 1 doi: 10.1016/S0895-4356(96)00277-6 – ident: 6 doi: 10.1007/BF03032294 |
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Title | Surveillance of Methicillin-Resistant Staphylococcus aureus: Cluster Analysis for Minimum Inhibitory Concentration Typing Using Spreadsheet Software |
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