Surveillance of Methicillin-Resistant Staphylococcus aureus: Cluster Analysis for Minimum Inhibitory Concentration Typing Using Spreadsheet Software

We have reported an accuracy of 73% using cluster analysis on drug sensitivity using the SPSSTM (Method S) compared to genotyping for methicillin-resistant Staphylococcus aureus (MRSA). Here we studied the efficacy of cluster analysis using ExcelTM with free additional software (Method E) on multi-d...

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Published inJapanese Journal of Infection Prevention and Control Vol. 27; no. 5; pp. 323 - 328
Main Authors UENO, Takako, MATSUBARA, Nobuo, YAMASHITA, Akihisa, YOSHIDA, Junichi, ISHIMARU, Toshiyuki, KIKUCHI, Tetsuya, ASANO, Ikuyo, HIRATA, Noriko
Format Journal Article
LanguageEnglish
Japanese
Published Japanese Society for Infection Prevention and Control 2012
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ISSN1882-532X
1883-2407
1883-2407
DOI10.4058/jsei.27.323

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Abstract We have reported an accuracy of 73% using cluster analysis on drug sensitivity using the SPSSTM (Method S) compared to genotyping for methicillin-resistant Staphylococcus aureus (MRSA). Here we studied the efficacy of cluster analysis using ExcelTM with free additional software (Method E) on multi-drug susceptibility compared to Method S. Subjects were MRSA strains first isolated in an individual. Cluster analysis was performed in (1) 71 strains using the same 11 drugs used in our previous study, (2) 70 strains using 15 drugs for the 2011 series, and (3) 70 strains using 13 drugs for the 2005 series suspected of cross infection. (1) Method E typed completely as did Method S. (2) Method E showed sensitivity, specificity, and accuracy of 97.6%, 92.9%, and 95.7% compared to Method S. (3) Method S typed four strains in one cluster separately from those of hospital personnel, indicating cross propagation rather than transmission via the staff. Hand-to-hand infection control was enforced with success. Method E showed identical typing with Method S. Methods E and S allowed typing in about three days. Therefore, typing using Method E may have advantages in analyzing MRSA cross infection, enabling rapid intervention.
AbstractList We have reported an accuracy of 73% using cluster analysis on drug sensitivity using the SPSSTM (Method S) compared to genotyping for methicillin-resistant Staphylococcus aureus (MRSA). Here we studied the efficacy of cluster analysis using ExcelTM with free additional software (Method E) on multi-drug susceptibility compared to Method S. Subjects were MRSA strains first isolated in an individual. Cluster analysis was performed in (1) 71 strains using the same 11 drugs used in our previous study, (2) 70 strains using 15 drugs for the 2011 series, and (3) 70 strains using 13 drugs for the 2005 series suspected of cross infection. (1) Method E typed completely as did Method S. (2) Method E showed sensitivity, specificity, and accuracy of 97.6%, 92.9%, and 95.7% compared to Method S. (3) Method S typed four strains in one cluster separately from those of hospital personnel, indicating cross propagation rather than transmission via the staff. Hand-to-hand infection control was enforced with success. Method E showed identical typing with Method S. Methods E and S allowed typing in about three days. Therefore, typing using Method E may have advantages in analyzing MRSA cross infection, enabling rapid intervention.
Author ISHIMARU, Toshiyuki
YAMASHITA, Akihisa
ASANO, Ikuyo
MATSUBARA, Nobuo
UENO, Takako
YOSHIDA, Junichi
HIRATA, Noriko
KIKUCHI, Tetsuya
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Cites_doi 10.1128/JCM.29.12.2690-2695.1991
10.1016/S1201-9712(01)90072-4
10.1016/S0895-4356(96)00277-6
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6) 佐藤正一,齊藤佳子,佐藤洋子,澤田恭子:MRSAにおける薬剤感受性クラスター解析およびパルスフィールド・ゲル電気泳動パターンの関連性.医学検査 2008; 57(3): 229–35.
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5) Ichiyama S, Ohta M, Shimokata K, Kato N, Takeuchi J: Genomic DNA fingerprinting by pulsed-field gel electrophoresis as an epidemiological marker for study of nosocomial infections caused by methicillin-resistant Staphylococcus aureus. J Clin Microbiol 1991; 29: 2690–5.
8) Yoshida J, Kirikae T, Yamanaka N, Suzuki H, Onzuka T, Hisahara M, et al.: Evidence-based infection control in thoracic surgery. Jpn J Thorac Cardiovasc Surg 2002; 50(7): 273–9.
3) Yoshida J, Ishimaru T, Kawano S, Akao M: An electronic system combining MIC2000 (TM) and antibiogram cluster analysis for surveillance of methicillin-resistant Staphylococcus aureus in hospitals. Jpn J Infect Dis 2000; 53: 79–81.
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– reference: 8) Yoshida J, Kirikae T, Yamanaka N, Suzuki H, Onzuka T, Hisahara M, et al.: Evidence-based infection control in thoracic surgery. Jpn J Thorac Cardiovasc Surg 2002; 50(7): 273–9.
– reference: 7) 松原伸夫:ICU・手術室でのスリッパ履き替えの評価.山口臨技 2002; 26(3): 4–10.
– reference: 3) Yoshida J, Ishimaru T, Kawano S, Akao M: An electronic system combining MIC2000 (TM) and antibiogram cluster analysis for surveillance of methicillin-resistant Staphylococcus aureus in hospitals. Jpn J Infect Dis 2000; 53: 79–81.
– reference: 5) Ichiyama S, Ohta M, Shimokata K, Kato N, Takeuchi J: Genomic DNA fingerprinting by pulsed-field gel electrophoresis as an epidemiological marker for study of nosocomial infections caused by methicillin-resistant Staphylococcus aureus. J Clin Microbiol 1991; 29: 2690–5.
– reference: 6) 佐藤正一,齊藤佳子,佐藤洋子,澤田恭子:MRSAにおける薬剤感受性クラスター解析およびパルスフィールド・ゲル電気泳動パターンの関連性.医学検査 2008; 57(3): 229–35.
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SubjectTerms Computer programs
methicillin-resistant Staphylococcus aureus (MRSA)
minimum inhibitory concentrations (MIC)
Staphylococcus aureus
surveillance
Title Surveillance of Methicillin-Resistant Staphylococcus aureus: Cluster Analysis for Minimum Inhibitory Concentration Typing Using Spreadsheet Software
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