Multi-target detection of oxidative stress biomarkers in quercetin and myricetin treated human red blood cells
Quercetin and myricetin are important dietary flavonoids with potential health benefits and interfere with reactive oxygen species metabolism. The objective of this study was multi-target spectroscopic analysis of oxidative stress biomarkers (malondialdehyde (MDA), glutathione (GSH) and sulfhydryl (...
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| Published in | RSC advances Vol. 6; no. 58; pp. 53195 - 5322 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
01.01.2016
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 2046-2069 2046-2069 |
| DOI | 10.1039/c6ra05121a |
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| Abstract | Quercetin and myricetin are important dietary flavonoids with potential health benefits and interfere with reactive oxygen species metabolism. The objective of this study was multi-target spectroscopic analysis of oxidative stress biomarkers (malondialdehyde (MDA), glutathione (GSH) and sulfhydryl (-SH) groups) in quercetin and myricetin treated red blood cells (RBCs) during human aging. The study was carried out on clinically relevant blood samples obtained from 105 healthy subjects between the ages of 18-82 years. The subjects were divided into three age groups, young (18-35 years), middle (36-60 years) and old (>60 years). Oxidative stress was induced
in vitro
by incubating RBCs with 10
−5
M
tert
-butyl hydroperoxide (
t
-BHP). The effects of flavonoids were evaluated by detecting MDA, GSH and -SH groups by co-incubating the RBCs in the presence of flavonoid (10
−8
M to 10
−5
M final concentration) and
t
-BHP. The GSH/GSSG ratios were estimated to demonstrate the antioxidant power of the RBCs. The results showed elevated MDA levels (
p
< 0.001) after incubation with
t
-BHP as compared to a control. The GSH and -SH groups significantly (
p
< 0.001) decreased when incubated with
t
-BHP.
In vitro
administration of both flavonoids significantly attenuated the deleterious effect of oxidative stress in erythrocytes from all age groups. We showed a significant (
p
< 0.0001) negative correlation (
r
= −0.8334) between GSH/GSSG during human aging. We believe that these findings are novel and will help in the fast screening of new chemical molecules which may help against oxidative stress in RBCs, and thereby have tremendous scope in medical diagnostics and therapeutics.
Quercetin and myricetin help against oxidative stress in human red blood cells during aging, thereby has tremendous scope in medical diagnostics and therapeutics. |
|---|---|
| AbstractList | Quercetin and myricetin are important dietary flavonoids with potential health benefits and interfere with reactive oxygen species metabolism. The objective of this study was multi-target spectroscopic analysis of oxidative stress biomarkers (malondialdehyde (MDA), glutathione (GSH) and sulfhydryl (–SH) groups) in quercetin and myricetin treated red blood cells (RBCs) during human aging. The study was carried out on clinically relevant blood samples obtained from 105 healthy subjects between the ages of 18–82 years. The subjects were divided into three age groups, young (18–35 years), middle (36–60 years) and old (>60 years). Oxidative stress was induced in vitro by incubating RBCs with 10⁻⁵ M tert-butyl hydroperoxide (t-BHP). The effects of flavonoids were evaluated by detecting MDA, GSH and –SH groups by co-incubating the RBCs in the presence of flavonoid (10⁻⁸ M to 10⁻⁵ M final concentration) and t-BHP. The GSH/GSSG ratios were estimated to demonstrate the antioxidant power of the RBCs. The results showed elevated MDA levels (p < 0.001) after incubation with t-BHP as compared to a control. The GSH and –SH groups significantly (p < 0.001) decreased when incubated with t-BHP. In vitro administration of both flavonoids significantly attenuated the deleterious effect of oxidative stress in erythrocytes from all age groups. We showed a significant (p < 0.0001) negative correlation (r = −0.8334) between GSH/GSSG during human aging. We believe that these findings are novel and will help in the fast screening of new chemical molecules which may help against oxidative stress in RBCs, and thereby have tremendous scope in medical diagnostics and therapeutics. Quercetin and myricetin are important dietary flavonoids with potential health benefits and interfere with reactive oxygen species metabolism. The objective of this study was multi-target spectroscopic analysis of oxidative stress biomarkers (malondialdehyde (MDA), glutathione (GSH) and sulfhydryl (-SH) groups) in quercetin and myricetin treated red blood cells (RBCs) during human aging. The study was carried out on clinically relevant blood samples obtained from 105 healthy subjects between the ages of 18-82 years. The subjects were divided into three age groups, young (18-35 years), middle (36-60 years) and old (>60 years). Oxidative stress was induced in vitro by incubating RBCs with 10 −5 M tert -butyl hydroperoxide ( t -BHP). The effects of flavonoids were evaluated by detecting MDA, GSH and -SH groups by co-incubating the RBCs in the presence of flavonoid (10 −8 M to 10 −5 M final concentration) and t -BHP. The GSH/GSSG ratios were estimated to demonstrate the antioxidant power of the RBCs. The results showed elevated MDA levels ( p < 0.001) after incubation with t -BHP as compared to a control. The GSH and -SH groups significantly ( p < 0.001) decreased when incubated with t -BHP. In vitro administration of both flavonoids significantly attenuated the deleterious effect of oxidative stress in erythrocytes from all age groups. We showed a significant ( p < 0.0001) negative correlation ( r = −0.8334) between GSH/GSSG during human aging. We believe that these findings are novel and will help in the fast screening of new chemical molecules which may help against oxidative stress in RBCs, and thereby have tremendous scope in medical diagnostics and therapeutics. Quercetin and myricetin help against oxidative stress in human red blood cells during aging, thereby has tremendous scope in medical diagnostics and therapeutics. Quercetin and myricetin are important dietary flavonoids with potential health benefits and interfere with reactive oxygen species metabolism. The objective of this study was multi-target spectroscopic analysis of oxidative stress biomarkers (malondialdehyde (MDA), glutathione (GSH) and sulfhydryl (–SH) groups) in quercetin and myricetin treated red blood cells (RBCs) during human aging. The study was carried out on clinically relevant blood samples obtained from 105 healthy subjects between the ages of 18–82 years. The subjects were divided into three age groups, young (18–35 years), middle (36–60 years) and old (>60 years). Oxidative stress was induced in vitro by incubating RBCs with 10 −5 M tert -butyl hydroperoxide ( t -BHP). The effects of flavonoids were evaluated by detecting MDA, GSH and –SH groups by co-incubating the RBCs in the presence of flavonoid (10 −8 M to 10 −5 M final concentration) and t -BHP. The GSH/GSSG ratios were estimated to demonstrate the antioxidant power of the RBCs. The results showed elevated MDA levels ( p < 0.001) after incubation with t -BHP as compared to a control. The GSH and –SH groups significantly ( p < 0.001) decreased when incubated with t -BHP. In vitro administration of both flavonoids significantly attenuated the deleterious effect of oxidative stress in erythrocytes from all age groups. We showed a significant ( p < 0.0001) negative correlation ( r = −0.8334) between GSH/GSSG during human aging. We believe that these findings are novel and will help in the fast screening of new chemical molecules which may help against oxidative stress in RBCs, and thereby have tremendous scope in medical diagnostics and therapeutics. |
| Author | Nagotu, Shirisha Maurya, Pawan Kumar Kumar, Prabhanshu Chandra, Pranjal Chand, Subhash |
| AuthorAffiliation | Department of Psychiatry Indian Institute of Technology Amity Institute of Biotechnology Interdisciplinary Laboratory for Clinical Neuroscience (LiNC) Indian Institute of Technology-Guwahati Universidade Federal de Sao Paulo - UNIFESP Department of Biochemical Engineering & Biotechnology Amity University Uttar Pradesh Department of Biosciences and Bioengineering |
| AuthorAffiliation_xml | – sequence: 0 name: Department of Biochemical Engineering & Biotechnology – sequence: 0 name: Amity Institute of Biotechnology – sequence: 0 name: Department of Biosciences and Bioengineering – sequence: 0 name: Indian Institute of Technology-Guwahati – sequence: 0 name: Interdisciplinary Laboratory for Clinical Neuroscience (LiNC) – sequence: 0 name: Amity University Uttar Pradesh – sequence: 0 name: Universidade Federal de Sao Paulo - UNIFESP – sequence: 0 name: Indian Institute of Technology – sequence: 0 name: Department of Psychiatry |
| Author_xml | – sequence: 1 givenname: Pawan Kumar surname: Maurya fullname: Maurya, Pawan Kumar – sequence: 2 givenname: Prabhanshu surname: Kumar fullname: Kumar, Prabhanshu – sequence: 3 givenname: Shirisha surname: Nagotu fullname: Nagotu, Shirisha – sequence: 4 givenname: Subhash surname: Chand fullname: Chand, Subhash – sequence: 5 givenname: Pranjal surname: Chandra fullname: Chandra, Pranjal |
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| CitedBy_id | crossref_primary_10_1007_s00232_021_00200_2 crossref_primary_10_1007_s13205_021_03087_x crossref_primary_10_1016_j_microc_2022_107615 crossref_primary_10_3390_nu10070817 crossref_primary_10_1155_2019_3746326 crossref_primary_10_1007_s13205_019_1807_4 crossref_primary_10_1007_s13205_018_1524_4 crossref_primary_10_1007_s13205_019_1577_z crossref_primary_10_1016_j_cccb_2024_100226 crossref_primary_10_3390_molecules27082498 crossref_primary_10_3233_JAD_170969 crossref_primary_10_1039_C6RA23411A |
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| SubjectTerms | antioxidant activity biomarkers blood sampling diagnostic techniques erythrocytes glutathione humans malondialdehyde metabolism myricetin oxidative stress quercetin reactive oxygen species screening spectral analysis therapeutics |
| Title | Multi-target detection of oxidative stress biomarkers in quercetin and myricetin treated human red blood cells |
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