Protective Effect of Curcumin on Endotoxin-induced Acute Lung Injury in Rats

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 26; no. 6; pp. 678 - 681
• Main Author: 连庆泉 李兴旺 尚游 姚尚龙 马利 金胜威
• Format: Journal Article
• Language: English
• Published: China Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, China%Department of Anesthesiology, the Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical College,Wenzhou 325027, China 2006
• Subjects: Acute Lung Injury - chemically induced; Acute Lung Injury - drug therapy; Acute Lung Injury - metabolism; Animals; Chemokine CXCL1 - metabolism; Curcumin - therapeutic use; Disease Models, Animal; Endotoxins - toxicity; Lung - metabolism; Lung - pathology; Male; Neutrophils - cytology; Rats; Rats, Wistar; 保护作用; 内毒素; 急性肺损伤; CINC-1; lipopolysaccharide; acute lung injury; neutrophils
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-006-0613-5

To investigate the protective effect of curcumin on endotoxin-induced acute lung injury in rats, and explore the underlying mechanisms, 24 male Wistar rats were randomly divided into 4 experimental groups： sham-vehicle （S）, sham-curcumin （C）, lipopolysaccharide （LPS）-vehicle （L）, and curcumin-lipopolysaccharide （C-L） groups. The wet/dry （W/D） weight ratio of the lung and bronchoalveolar lavage （BAL） fluid protein content were used as measures of lung injury. Neutrophil recruitment and activation were evaluated by BAL fluid cellularity and myeloperoxidase （MPO） activity in cell-free BAL and lung tissue. The levels of cytokine-induced neutrophil chemoattractant-1 （CINC-1） in lung tissues were measured by ELISA. were observed by using the HE staining. Our results the wet/dry weight ratio and protein content in BALE The histopathological changes of lung tissues showed that lung injury parameters, including were significantly higher in the L group than in the S group （P〈0.01）. In the L group, higher numbers of neutrophils and greater MPO activity in cell-free BAL and lung homogenates were observed when compared with the S group （P〈0.01）. There was a marked increase in CINC-1 levels in lung tissues in response to LPS challenge （P〈0.01, L group vs S group）. Curcumin pretreatment significantly attenuated LPS-induced changes in these indices. LPS caused extensive morphological lung damage, which was also lessened after curcumin pretreatment. All the above-mentioned parameters in the C group were not significantly different from those of the S group. It is concluded that curcumin pretreatment attenuates LPS-induced lung injury in rats. This beneficial effect of curcumin may involves, in part, inhibition of neutrophilic recruitment and activity, possibly through inhibition of lung CINC-1 expression.