Spinal Stroke: Outcome Attenuation by Erythropoietin and Carbamylated Erythropoietin and Its Prediction by Sphingosine-1-Phosphate Serum Levels in Mice
Spinal strokes may be associated with tremendous spinal cord injury. Erythropoietin (EPO) improves the neurological outcome of animals after spinal cord ischemia (SCI) and its effects on ischemia-induced endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) are considered possibl...
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| Published in | International journal of molecular sciences Vol. 23; no. 17; p. 9558 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Basel
MDPI AG
23.08.2022
MDPI |
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| Online Access | Get full text |
| ISSN | 1422-0067 1661-6596 1422-0067 |
| DOI | 10.3390/ijms23179558 |
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| Abstract | Spinal strokes may be associated with tremendous spinal cord injury. Erythropoietin (EPO) improves the neurological outcome of animals after spinal cord ischemia (SCI) and its effects on ischemia-induced endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) are considered possible molecular mechanisms. Furthermore, sphingosin-1-phosphate (S1P) is suggested to correlate with SCI. In this study, the effect of recombinant human EPO (rhEPO) and carbamylated EPO (cEPO-Fc) on the outcome of mice after SCI and a prognostic value of S1P were investigated. SCI was induced in 12-month-old male mice by thoracic aortal cross-clamping after administration of rhEPO, cEPO-Fc, or a control. The locomotory behavior of mice was evaluated by the Basso mouse scale and S1P serum levels were measured by liquid chromatography-tandem mass spectrometry. The spinal cord was examined histologically and the expressions of key UPR proteins (ATF6, PERK, and IRE1a, caspase-12) were analyzed utilizing immunohistochemistry and real-time quantitative polymerase chain reaction. RhEPO and cEPO-Fc significantly improved outcomes after SCI. The expression of caspase-12 significantly increased in the control group within the first 24 h of reperfusion. Animals with better locomotory behavior had significantly higher serum levels of S1P. Our data indicate that rhEPO and cEPO-Fc have protective effects on the clinical outcome and neuronal tissue of mice after SCI and that the ER is involved in the molecular mechanisms. Moreover, serum S1P may predict the severity of impairment after SCI. |
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| AbstractList | Spinal strokes may be associated with tremendous spinal cord injury. Erythropoietin (EPO) improves the neurological outcome of animals after spinal cord ischemia (SCI) and its effects on ischemia-induced endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) are considered possible molecular mechanisms. Furthermore, sphingosin-1-phosphate (S1P) is suggested to correlate with SCI. In this study, the effect of recombinant human EPO (rhEPO) and carbamylated EPO (cEPO-Fc) on the outcome of mice after SCI and a prognostic value of S1P were investigated. SCI was induced in 12-month-old male mice by thoracic aortal cross-clamping after administration of rhEPO, cEPO-Fc, or a control. The locomotory behavior of mice was evaluated by the Basso mouse scale and S1P serum levels were measured by liquid chromatography-tandem mass spectrometry. The spinal cord was examined histologically and the expressions of key UPR proteins (ATF6, PERK, and IRE1a, caspase-12) were analyzed utilizing immunohistochemistry and real-time quantitative polymerase chain reaction. RhEPO and cEPO-Fc significantly improved outcomes after SCI. The expression of caspase-12 significantly increased in the control group within the first 24 h of reperfusion. Animals with better locomotory behavior had significantly higher serum levels of S1P. Our data indicate that rhEPO and cEPO-Fc have protective effects on the clinical outcome and neuronal tissue of mice after SCI and that the ER is involved in the molecular mechanisms. Moreover, serum S1P may predict the severity of impairment after SCI. Spinal strokes may be associated with tremendous spinal cord injury. Erythropoietin (EPO) improves the neurological outcome of animals after spinal cord ischemia (SCI) and its effects on ischemia-induced endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) are considered possible molecular mechanisms. Furthermore, sphingosin-1-phosphate (S1P) is suggested to correlate with SCI. In this study, the effect of recombinant human EPO (rhEPO) and carbamylated EPO (cEPO-Fc) on the outcome of mice after SCI and a prognostic value of S1P were investigated. SCI was induced in 12-month-old male mice by thoracic aortal cross-clamping after administration of rhEPO, cEPO-Fc, or a control. The locomotory behavior of mice was evaluated by the Basso mouse scale and S1P serum levels were measured by liquid chromatography-tandem mass spectrometry. The spinal cord was examined histologically and the expressions of key UPR proteins (ATF6, PERK, and IRE1a, caspase-12) were analyzed utilizing immunohistochemistry and real-time quantitative polymerase chain reaction. RhEPO and cEPO-Fc significantly improved outcomes after SCI. The expression of caspase-12 significantly increased in the control group within the first 24 h of reperfusion. Animals with better locomotory behavior had significantly higher serum levels of S1P. Our data indicate that rhEPO and cEPO-Fc have protective effects on the clinical outcome and neuronal tissue of mice after SCI and that the ER is involved in the molecular mechanisms. Moreover, serum S1P may predict the severity of impairment after SCI.Spinal strokes may be associated with tremendous spinal cord injury. Erythropoietin (EPO) improves the neurological outcome of animals after spinal cord ischemia (SCI) and its effects on ischemia-induced endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) are considered possible molecular mechanisms. Furthermore, sphingosin-1-phosphate (S1P) is suggested to correlate with SCI. In this study, the effect of recombinant human EPO (rhEPO) and carbamylated EPO (cEPO-Fc) on the outcome of mice after SCI and a prognostic value of S1P were investigated. SCI was induced in 12-month-old male mice by thoracic aortal cross-clamping after administration of rhEPO, cEPO-Fc, or a control. The locomotory behavior of mice was evaluated by the Basso mouse scale and S1P serum levels were measured by liquid chromatography-tandem mass spectrometry. The spinal cord was examined histologically and the expressions of key UPR proteins (ATF6, PERK, and IRE1a, caspase-12) were analyzed utilizing immunohistochemistry and real-time quantitative polymerase chain reaction. RhEPO and cEPO-Fc significantly improved outcomes after SCI. The expression of caspase-12 significantly increased in the control group within the first 24 h of reperfusion. Animals with better locomotory behavior had significantly higher serum levels of S1P. Our data indicate that rhEPO and cEPO-Fc have protective effects on the clinical outcome and neuronal tissue of mice after SCI and that the ER is involved in the molecular mechanisms. Moreover, serum S1P may predict the severity of impairment after SCI. |
| Author | Schelzig, Hubert Koepke, Leon-Gordian Daum, Guenter Simon, Florian Vcelar, Brigitta Ibing, Wiebke Schwedhelm, Edzard Oberhuber, Alexander |
| AuthorAffiliation | 4 Clinic for Vascular and Endovascular Surgery, University Hospital Duesseldorf, Heinrich-Heine-University Duesseldorf, 40225 Düsseldorf, Germany 3 German Center for Cardiovascular Research (DZHK e.V.), Partner Site Hamburg/Kiel/Lübeck, 20251 Hamburg, Germany 1 Department of Trauma Surgery and Orthopedic Surgery, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany 6 Department of Vascular Medicine, University Heart and Vascular Center Hamburg, 20251 Hamburg, Germany 7 Polymun Scientific Immunbiologische Forschung GmbH, 3400 Klosterneuburg, Austria 5 Clinic for Vascular and Endovascular Surgery, University Hospital Münster, 48149 Münster, Germany 2 Institute for Clinical Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany |
| AuthorAffiliation_xml | – name: 3 German Center for Cardiovascular Research (DZHK e.V.), Partner Site Hamburg/Kiel/Lübeck, 20251 Hamburg, Germany – name: 5 Clinic for Vascular and Endovascular Surgery, University Hospital Münster, 48149 Münster, Germany – name: 1 Department of Trauma Surgery and Orthopedic Surgery, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany – name: 6 Department of Vascular Medicine, University Heart and Vascular Center Hamburg, 20251 Hamburg, Germany – name: 2 Institute for Clinical Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany – name: 7 Polymun Scientific Immunbiologische Forschung GmbH, 3400 Klosterneuburg, Austria – name: 4 Clinic for Vascular and Endovascular Surgery, University Hospital Duesseldorf, Heinrich-Heine-University Duesseldorf, 40225 Düsseldorf, Germany |
| Author_xml | – sequence: 1 givenname: Leon-Gordian orcidid: 0000-0003-3966-8537 surname: Koepke fullname: Koepke, Leon-Gordian – sequence: 2 givenname: Edzard orcidid: 0000-0003-3597-6276 surname: Schwedhelm fullname: Schwedhelm, Edzard – sequence: 3 givenname: Wiebke surname: Ibing fullname: Ibing, Wiebke – sequence: 4 givenname: Alexander orcidid: 0000-0002-6322-7152 surname: Oberhuber fullname: Oberhuber, Alexander – sequence: 5 givenname: Guenter surname: Daum fullname: Daum, Guenter – sequence: 6 givenname: Brigitta surname: Vcelar fullname: Vcelar, Brigitta – sequence: 7 givenname: Hubert surname: Schelzig fullname: Schelzig, Hubert – sequence: 8 givenname: Florian orcidid: 0000-0001-5743-8554 surname: Simon fullname: Simon, Florian |
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| Cites_doi | 10.1083/jcb.150.4.887 10.1016/j.jtcvs.2010.09.017 10.1016/S1534-5807(02)00203-4 10.1111/jcmm.14688 10.1007/s00772-018-0388-6 10.1016/j.jvs.2015.10.011 10.1111/j.1582-4934.2011.01489.x 10.1074/jbc.M204973200 10.1006/abio.1999.4085 10.1634/stemcells.2006-0223 10.1161/STROKEAHA.120.029071 10.1007/s12035-017-0858-y 10.1007/s11064-020-03088-x 10.1161/CIRCRESAHA.120.316711 10.1016/j.jtcvs.2014.12.025 10.1097/00004647-200202000-00001 10.1016/j.ajpath.2011.12.012 10.1111/j.1471-4159.2004.02703.x 10.1007/s12031-020-01691-9 10.1097/00000658-198607000-00005 10.1620/tjem.219.295 10.1016/j.jss.2018.07.021 10.1016/S0968-0004(01)01908-9 10.1038/47513 10.1161/STROKEAHA.120.033414 10.1074/jbc.M010677200 10.1016/S1078-5884(97)80208-0 10.1097/SHK.0b013e3181890733 10.1016/j.jvs.2010.06.172 10.1016/j.cca.2017.01.029 10.1016/j.jtcvs.2005.01.007 10.1089/neu.2008.0840 10.1097/CCM.0b013e31817d7912 10.1016/S0022-5223(99)70367-3 10.1089/neu.2006.23.635 10.1016/j.pep.2006.05.018 10.1038/sj.cdd.4401378 10.1016/j.bbrc.2018.03.065 |
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| References | Kimura (ref_16) 2007; 25 Moritz (ref_40) 2017; 468 Winer (ref_39) 1999; 270 Dong (ref_28) 2015; 8 Basso (ref_37) 2006; 23 Safi (ref_2) 1997; 14 Shen (ref_11) 2002; 3 Mizukami (ref_29) 2010; 52 Rodrigues (ref_35) 2018; 55 Smith (ref_7) 2011; 141 Chen (ref_30) 2020; 45 Etz (ref_4) 2015; 149 Wang (ref_31) 2020; 51 Sakurai (ref_21) 2005; 130 Norimatsu (ref_18) 2012; 180 Simon (ref_38) 2018; 23 Schriebl (ref_36) 2006; 49 Hirano (ref_20) 2012; 16 DeGracia (ref_10) 2002; 22 Schwedhelm (ref_13) 2021; 52 DeGracia (ref_8) 2004; 91 Simon (ref_6) 2009; 31 Simon (ref_5) 2008; 36 Nakagawa (ref_25) 2000; 150 Tang (ref_34) 2018; 498 Zhang (ref_17) 2009; 219 Heurteaux (ref_3) 1999; 117 Morishima (ref_26) 2002; 277 Rao (ref_12) 2004; 11 Jones (ref_15) 2016; 8 Lee (ref_9) 2001; 26 Nitzsche (ref_14) 2021; 128 Lee (ref_33) 2009; 26 Zhao (ref_27) 2019; 23 Yamanaka (ref_22) 2019; 233 Svensson (ref_1) 1986; 204 Nakagawa (ref_23) 2000; 403 Yoneda (ref_24) 2001; 276 Simon (ref_19) 2016; 64 Ji (ref_32) 2021; 71 |
| References_xml | – volume: 150 start-page: 887 year: 2000 ident: ref_25 article-title: Cross-talk between two cysteine protease families. Activation of caspase-12 by calpain in apoptosis publication-title: J. Cell Biol. doi: 10.1083/jcb.150.4.887 – volume: 141 start-page: 256 year: 2011 ident: ref_7 article-title: Attenuation of spinal cord ischemia and reperfusion injury by erythropoietin publication-title: J. Thorac. Cardiovasc. Surg. doi: 10.1016/j.jtcvs.2010.09.017 – volume: 3 start-page: 99 year: 2002 ident: ref_11 article-title: ER stress regulation of ATF6 localization by dissociation of BiP/GRP78 binding and unmasking of Golgi localization signals publication-title: Dev. Cell doi: 10.1016/S1534-5807(02)00203-4 – volume: 23 start-page: 8173 year: 2019 ident: ref_27 article-title: Dexmedetomidine attenuates neuronal injury after spinal cord ischaemia-reperfusion injury by targeting the CNPY2-endoplasmic reticulum stress signalling publication-title: J. Cell Mol. Med. doi: 10.1111/jcmm.14688 – volume: 23 start-page: 261 year: 2018 ident: ref_38 article-title: Immunhistochemische Färbungen—Ein möglicher Lösungsweg publication-title: Gefässchirurgie doi: 10.1007/s00772-018-0388-6 – volume: 64 start-page: 1797 year: 2016 ident: ref_19 article-title: Erythropoietin preconditioning improves clinical and histologic outcome in an acute spinal cord ischemia and reperfusion rabbit model publication-title: J. Vasc. Surg. doi: 10.1016/j.jvs.2015.10.011 – volume: 16 start-page: 1792 year: 2012 ident: ref_20 article-title: Erythropoietin attenuates the sequels of ischaemic spinal cord injury with enhanced recruitment of CD34+ cells in mice publication-title: J. Cell. Mol. Med. doi: 10.1111/j.1582-4934.2011.01489.x – volume: 277 start-page: 34287 year: 2002 ident: ref_26 article-title: An endoplasmic reticulum stress-specific caspase cascade in apoptosis. Cytochrome c-independent activation of caspase-9 by caspase-12 publication-title: J. Biol. Chem. doi: 10.1074/jbc.M204973200 – volume: 270 start-page: 41 year: 1999 ident: ref_39 article-title: Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro publication-title: Anal. Biochem. doi: 10.1006/abio.1999.4085 – volume: 25 start-page: 115 year: 2007 ident: ref_16 article-title: Essential roles of sphingosine 1-phosphate/S1P1 receptor axis in the migration of neural stem cells toward a site of spinal cord injury publication-title: Stem Cells doi: 10.1634/stemcells.2006-0223 – volume: 51 start-page: 1570 year: 2020 ident: ref_31 article-title: PERK (Protein Kinase RNA-Like ER Kinase) Branch of the Unfolded Protein Response Confers Neuroprotection in Ischemic Stroke by Suppressing Protein Synthesis publication-title: Stroke doi: 10.1161/STROKEAHA.120.029071 – volume: 55 start-page: 6436 year: 2018 ident: ref_35 article-title: Biomarkers in Spinal Cord Injury: From Prognosis to Treatment publication-title: Mol. Neurobiol. doi: 10.1007/s12035-017-0858-y – volume: 45 start-page: 2302 year: 2020 ident: ref_30 article-title: Novel Role of HAX-1 in Neurons Protection After Spinal Cord Injury Involvement of IRE-1 publication-title: Neurochem. Res. doi: 10.1007/s11064-020-03088-x – volume: 128 start-page: 363 year: 2021 ident: ref_14 article-title: Endothelial S1P1 Signaling Counteracts Infarct Expansion in Ischemic Stroke publication-title: Circ. Res. doi: 10.1161/CIRCRESAHA.120.316711 – volume: 149 start-page: 1074 year: 2015 ident: ref_4 article-title: First-in-man endovascular preconditioning of the paraspinal collateral network by segmental artery coil embolization to prevent ischemic spinal cord injury publication-title: J. Thorac. Cardiovasc. Surg. doi: 10.1016/j.jtcvs.2014.12.025 – volume: 22 start-page: 127 year: 2002 ident: ref_10 article-title: Molecular pathways of protein synthesis inhibition during brain reperfusion: Implications for neuronal survival or death publication-title: J. Cereb. Blood Flow Metab. doi: 10.1097/00004647-200202000-00001 – volume: 180 start-page: 1625 year: 2012 ident: ref_18 article-title: FTY720 improves functional recovery after spinal cord injury by primarily nonimmunomodulatory mechanisms publication-title: Am. J. Pathol. doi: 10.1016/j.ajpath.2011.12.012 – volume: 91 start-page: 1 year: 2004 ident: ref_8 article-title: Cerebral ischemia and the unfolded protein response publication-title: J. Neurochem. doi: 10.1111/j.1471-4159.2004.02703.x – volume: 71 start-page: 734 year: 2021 ident: ref_32 article-title: Activating Transcription Factor 6 Contributes to Functional Recovery After Spinal Cord Injury in Adult Zebrafish publication-title: J. Mol. Neurosci. doi: 10.1007/s12031-020-01691-9 – volume: 204 start-page: 38 year: 1986 ident: ref_1 article-title: Cross-clamping of the thoracic aorta. Influence of aortic shunts, laminectomy, papaverine, calcium channel blocker, allopurinol, and superoxide dismutase on spinal cord blood flow and paraplegia in baboons publication-title: Ann. Surg. doi: 10.1097/00000658-198607000-00005 – volume: 219 start-page: 295 year: 2009 ident: ref_17 article-title: Treatment with immunosuppressants FTY720 and tacrolimus promotes functional recovery after spinal cord injury in rats publication-title: Tohoku J. Exp. Med. doi: 10.1620/tjem.219.295 – volume: 233 start-page: 124 year: 2019 ident: ref_22 article-title: Synergetic Induction of NGF With Diazoxide and Erythropoietin Attenuates Spinal Cord Ischemic Injury publication-title: J. Surg. Res. doi: 10.1016/j.jss.2018.07.021 – volume: 26 start-page: 504 year: 2001 ident: ref_9 article-title: The glucose-regulated proteins: Stress induction and clinical applications publication-title: Trends Biochem. Sci. doi: 10.1016/S0968-0004(01)01908-9 – volume: 403 start-page: 98 year: 2000 ident: ref_23 article-title: Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta publication-title: Nature doi: 10.1038/47513 – volume: 52 start-page: 3901 year: 2021 ident: ref_13 article-title: Serum Sphingosine-1-Phosphate Levels Are Associated With Severity and Outcome in Patients With Cerebral Ischemia publication-title: Stroke doi: 10.1161/STROKEAHA.120.033414 – volume: 276 start-page: 13935 year: 2001 ident: ref_24 article-title: Activation of caspase-12, an endoplastic reticulum (ER) resident caspase, through tumor necrosis factor receptor-associated factor 2-dependent mechanism in response to the ER stress publication-title: J. Biol. Chem. doi: 10.1074/jbc.M010677200 – volume: 14 start-page: 118 year: 1997 ident: ref_2 article-title: Cerebral spinal fluid drainage and distal aortic perfusion decrease the incidence of neurological deficit: The results of 343 descending and thoracoabdominal aortic aneurysm repairs publication-title: Eur. J. Vasc. Endovasc. Surg. Off. J. Eur. Soc. Vasc. Surg. doi: 10.1016/S1078-5884(97)80208-0 – volume: 31 start-page: 220 year: 2009 ident: ref_6 article-title: Beneficial effects of erythropoietin in models of shock and organ failure-nothing is simple and easy publication-title: Shock doi: 10.1097/SHK.0b013e3181890733 – volume: 52 start-page: 1580 year: 2010 ident: ref_29 article-title: Sodium 4-phenylbutyrate protects against spinal cord ischemia by inhibition of endoplasmic reticulum stress publication-title: J. Vasc. Surg. doi: 10.1016/j.jvs.2010.06.172 – volume: 468 start-page: 25 year: 2017 ident: ref_40 article-title: Reference intervals for serum sphingosine-1-phosphate in the population-based Study of Health in Pomerania publication-title: Clin. Chim. Acta doi: 10.1016/j.cca.2017.01.029 – volume: 130 start-page: 640 year: 2005 ident: ref_21 article-title: Endoplasmic reticulum stress induced in motor neurons by transient spinal cord ischemia in rabbits publication-title: J. Thorac. Cardiovasc. Surg. doi: 10.1016/j.jtcvs.2005.01.007 – volume: 26 start-page: 2335 year: 2009 ident: ref_33 article-title: FTY720 reduces inflammation and promotes functional recovery after spinal cord injury publication-title: J. Neurotrauma doi: 10.1089/neu.2008.0840 – volume: 36 start-page: 2143 year: 2008 ident: ref_5 article-title: Erythropoietin during porcine aortic balloon occlusion-induced ischemia/reperfusion injury publication-title: Crit. Care Med. doi: 10.1097/CCM.0b013e31817d7912 – volume: 8 start-page: 52 year: 2016 ident: ref_15 article-title: Sphingolipids in spinal cord injury publication-title: Int. J. Physiol. Pathophysiol. Pharmacol. – volume: 117 start-page: 881 year: 1999 ident: ref_3 article-title: Riluzole prevents ischemic spinal cord injury caused by aortic crossclamping publication-title: J. Thorac. Cardiovasc. Surg. doi: 10.1016/S0022-5223(99)70367-3 – volume: 23 start-page: 635 year: 2006 ident: ref_37 article-title: Basso Mouse Scale for locomotion detects differences in recovery after spinal cord injury in five common mouse strains publication-title: J. Neurotrauma doi: 10.1089/neu.2006.23.635 – volume: 8 start-page: 15871 year: 2015 ident: ref_28 article-title: Neuroprotective effects and impact on caspase-12 expression of tauroursodeoxycholic acid after acute spinal cord injury in rats publication-title: Int. J. Clin. Exp. Pathol. – volume: 49 start-page: 265 year: 2006 ident: ref_36 article-title: Biochemical characterization of rhEpo-Fc fusion protein expressed in CHO cells publication-title: Protein Expr. Purif. doi: 10.1016/j.pep.2006.05.018 – volume: 11 start-page: 372 year: 2004 ident: ref_12 article-title: Coupling endoplasmic reticulum stress to the cell death program publication-title: Cell Death Differ. doi: 10.1038/sj.cdd.4401378 – volume: 498 start-page: 830 year: 2018 ident: ref_34 article-title: S1P/S1PR3 signaling mediated proliferation of pericytes via Ras/pERK pathway and CAY10444 had beneficial effects on spinal cord injury publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/j.bbrc.2018.03.065 |
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| SubjectTerms | Animals Coronary vessels Endoplasmic reticulum Gene expression Ischemia Kinases Proteins Spinal cord |
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| Title | Spinal Stroke: Outcome Attenuation by Erythropoietin and Carbamylated Erythropoietin and Its Prediction by Sphingosine-1-Phosphate Serum Levels in Mice |
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