Transfection of B7—1 cDNA empowers antigen presentation of blood malignant cells for activation of anti—tumor T cells

• Published in: Chinese medical journal Vol. 116; no. 1; pp. 78 - 84
• Main Author: 克晓燕 贾丽萍 王晶 王德炳
• Format: Journal Article
• Language: English
• Published: China Department of Hematology, Third Hospital, Peking University, Beijing 100083, China%Institute of Blood Disease Research, People's Hospital, Peking University, Beijing 100083, China 2003
• Subjects: Antigen Presentation; Antigens, CD - analysis; B7-1 Antigen - analysis; B7-1 Antigen - physiology; B7-2 Antigen; B7－1转染; cDNA; Cytokines - genetics; Flow Cytometry; Hematologic Neoplasms - immunology; HLA-DR7 Antigen - physiology; Humans; K562 Cells; Lymphocyte Activation; Membrane Glycoproteins - analysis; RNA, Messenger - analysis; T-Lymphocytes - immunology; Transfection; U937 Cells; 恶性血液病细胞系; 抗原提呈; 抗肿瘤T－细胞; B7; tumor immunity; gene transfection
• ISSN: 0366-6999; 2542-5641

Ojbective To define roles of B7-1 co-stimulation factor expressed in human malignant cell lines in mediating anti-tumor T cell immune responses.Methods Examining human leucocyte antigen(HLA)and B7 expressions on 8 human blood malignancies cell lines by flow cytometry.Transtecting B7-1 gene to B7-1 negative(B7^-)Raji and B7^- Jurkat cell lines by liposome,and comparing the potencies of blood malignant cell lines in the induction of T cell activation by examination of T cell cytokine mRNAs before and after transtection using semi-quantitative reverse transcription polymerase chain reaction(RT-PCR). Results High level of HLA Ⅰ and Ⅱ molecules were expressed in most human blood malignant cell lines examined,and the co-stimulatory factor B7-2 was also highly expressed.In contrast,another member of B7 family:B7-1 was either not axpressed or very limitedly expressed in most of these hematopoietic malignant cell lines.Most importantly,transfection of B7-1 gene to B7^-.Raji and B7^-.Jurkat cell lines made these cell lines better antigen presenting cells for stimulation of anti-tumor T cell activation,which was demonstrated by up regulation of expression of T cell cytokines IL-2,IL-4 and INF-γ mRNAs after incubation of these tumor cells with T cells for 24h.Conclusions B7 co-stimulation plays an important role in anti-tumor immunity.Transfection of B7-1 gene to the human hematopoietic malignant cell lines that are deficient in the B7-1 expression empowers their antigen presentation potengy for activation of anti-tumor T cells.Our results suggested that repairing the deficiency of B7-1 co-stimulatory pathway in tumor cells might be a novel immunotherapeutic approach for human hematopoletic malignancies.