LncRNA LAMTOR5‐AS1 sponges miR‐210‐3p and regulates cervical cancer progression

• Published in: The journal of obstetrics and gynaecology research Vol. 48; no. 12; pp. 3171 - 3178
• Main Authors: Yang, Bo, Wang, Ziyi, Deng, Yuping, Xiao, Lingzhi, Zhang, Keqiang
• Format: Journal Article
• Language: English
• Published: Kyoto, Japan John Wiley & Sons Australia, Ltd 01.12.2022; Wiley Subscription Services, Inc
• Subjects: Biomarkers; Cell proliferation; Cervical cancer; Cervix; Cholecystokinin; lncRNA LAMTOR5‐AS1; miR‐210‐3p; Non-coding RNA; prognosis; progression; Reporter gene
• ISSN: 1341-8076; 1447-0756; 1447-0756
• DOI: 10.1111/jog.15439

Aim Cervical cancer has attracted increasing attention in recent years, and the incidence has shown a trend of younger age. Therefore, it is an effective method to regulate the progression of cervical cancer through new prognostic biomarkers. The purpose of this study was to evaluate the potential of lncRNA LAMTOR5‐AS1 (LAMTOR5‐AS1) as a prognostic biomarker and reveal its regulatory role in cervical cancer. Methods A total of 120 patients with cervical cancer were selected as research subjects to verify the prognostic effect of LAMTOR5‐AS1 in a series of experiments. The expression of LAMTOR5‐AS1 in cervical cancer tissues and cells was determined by polymerase chain reaction assay. The proliferation, migration, and invasion ability of cervical cancer cells were evaluated by Cell Counting Kit‐8 (CCK‐8) and Transwell assay. Luciferase reporter gene detection was used to determine the mechanism of LAMTOR5‐AS1 targeting miR‐210‐3p, and to reflect the prognostic value of LAMTOR5‐AS1 according to statistical methods. Results LAMTOR5‐AS1 decreased in cervical cancer tissues, while miR‐210‐3p expression increased. In the study of cervical cancer cells, it was found that the LAMTOR5‐AS1 sponge miR‐210‐3p was associated with the malignant progression of cervical cancer. Overexpression of LAMTOR5‐AS1 could effectively inhibit the development of cervical cancer cells and might be chosen as a prognostic biomarker of cervical cancer. Conclusions LAMTOR5‐AS1 sponges miR‐210‐3p and modulates the progression of cervical cancer, which predict the prognosis of cervical cancer patients.