Relationship between Apoptosis and E-cadherin Expression in Bronchial Epithelium of Smoking Mouse
To investigate whether apoptosis is associated with cell adhesion in bronchial epithelium,and whether it contributes to the kinetics of injury and repair of surface epithelia, this study was performed for E-cadherin expression by using immunohistochemistry technique and for apoptosis by TUNEL method...
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| Published in | Current medical science Vol. 23; no. 3; pp. 216 - 218 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
China
Department of Pathology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030
2003
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-0733 2096-5230 2523-899X |
| DOI | 10.1007/BF02829495 |
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| Abstract | To investigate whether apoptosis is associated with cell adhesion in bronchial epithelium,and whether it contributes to the kinetics of injury and repair of surface epithelia, this study was performed for E-cadherin expression by using immunohistochemistry technique and for apoptosis by TUNEL method. An animal model of smoking was used for this study. The results showed that epithelial cells with membrane anchored E-cadherin decreased remarkably at several time points during 6 months of exposure to smoke (P<0. 01) and then restored to normal level. This fluctuation was associated exclusively with the alteration in number of apoptotic cells (P<0. 01). There wasno significant difference in activation of nuclear transcription factor NF-kappa B among groups (P>0. 05). All these suggested that apoptosis is associated with E-cadherin expression in bronchial epithelium of smoking mouse. |
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| AbstractList | R318; To investigate whether apoptosis is associated with cell adhesion in bronchial epithelium,and whether it contributes to the kinetics of injury and repair of surface epithelia, this study wasperformed for E-cadherin expression by using immunohistochemistry technique and for apoptosis byTUNEL method. An animal model of smoking was used for this study. The results showed thatepithelial cells with membrane anchored E-cadherin decreased remarkably at several time points dur-ing 6 months of exposure to smoke (P＜0.01) and then restored to normal level. This fluctuationwas associated exclusively with the alteration in number of apoptotic cells (P＜0.01). There wasno significant difference in activation of nuclear transcription factor NF-kappa B among groups (P＞0.05). All these suggested that apoptosis is associated with E-cadherin expression in bronchial epi-thelium of smoking mouse. To investigate whether apoptosis is associated with cell adhesion in bronchial epithelium, and whether it contributes to the kinetics of injury and repair of surface epithelia, this study was performed for E-cadherin expression by using immunohistochemistry technique and for apoptosis by TUNEL method. An animal model of smoking was used for this study. The results showed that epithelial cells with membrane anchored E-cadherin decreased remarkably at several time points during 6 months of exposure to smoke (P < 0.01) and then restored to normal level. This fluctuation was associated exclusively with the alteration in number of apoptotic cells (P < 0.01). There was no significant difference in activation of nuclear transcription factor NF-kappa B among groups (P > 0.05). All these suggested that apoptosis is associated with E-cadherin expression in bronchial epithelium of smoking mouse. To investigate whether apoptosis is associated with cell adhesion in bronchial epithelium,and whether it contributes to the kinetics of injury and repair of surface epithelia, this study was performed for E-cadherin expression by using immunohistochemistry technique and for apoptosis by TUNEL method. An animal model of smoking was used for this study. The results showed that epithelial cells with membrane anchored E-cadherin decreased remarkably at several time points during 6 months of exposure to smoke (P<0. 01) and then restored to normal level. This fluctuation was associated exclusively with the alteration in number of apoptotic cells (P<0. 01). There wasno significant difference in activation of nuclear transcription factor NF-kappa B among groups (P>0. 05). All these suggested that apoptosis is associated with E-cadherin expression in bronchial epithelium of smoking mouse. To investigate whether apoptosis is associated with cell adhesion in bronchial epithelium, and whether it contributes to the kinetics of injury and repair of surface epithelia, this study was performed for E-cadherin expression by using immunohistochemistry technique and for apoptosis by TUNEL method. An animal model of smoking was used for this study. The results showed that epithelial cells with membrane anchored E-cadherin decreased remarkably at several time points during 6 months of exposure to smoke (P < 0.01) and then restored to normal level. This fluctuation was associated exclusively with the alteration in number of apoptotic cells (P < 0.01). There was no significant difference in activation of nuclear transcription factor NF-kappa B among groups (P > 0.05). All these suggested that apoptosis is associated with E-cadherin expression in bronchial epithelium of smoking mouse.To investigate whether apoptosis is associated with cell adhesion in bronchial epithelium, and whether it contributes to the kinetics of injury and repair of surface epithelia, this study was performed for E-cadherin expression by using immunohistochemistry technique and for apoptosis by TUNEL method. An animal model of smoking was used for this study. The results showed that epithelial cells with membrane anchored E-cadherin decreased remarkably at several time points during 6 months of exposure to smoke (P < 0.01) and then restored to normal level. This fluctuation was associated exclusively with the alteration in number of apoptotic cells (P < 0.01). There was no significant difference in activation of nuclear transcription factor NF-kappa B among groups (P > 0.05). All these suggested that apoptosis is associated with E-cadherin expression in bronchial epithelium of smoking mouse. |
| Author | 刘丽芳 袁永辉 李钫 刘红云 |
| AuthorAffiliation | DepartmentofPathology,SchoolofBasicMedicalSciences,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430030 |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/14526415$$D View this record in MEDLINE/PubMed |
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| Keywords | apoptosis E-cadherin bronchial epithelia smoking |
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| References | C Y Sasaki (BF02829495_CR3) 2000; 86 M Ozawa (BF02829495_CR4) 1998; 273 K Shinichi (BF02829495_CR6) 2000; 19 J J Peluso (BF02829495_CR1) 2001; 64 K Willert (BF02829495_CR5) 1998; 8 C J Vallorosi (BF02829495_CR2) 2000; 275 |
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| SubjectTerms | Animals Apoptosis Bronchi - metabolism Bronchi - pathology Cadherins - analysis Cadherins - biosynthesis E-钙粘素 Epithelial Cells - chemistry Epithelial Cells - metabolism Epithelial Cells - pathology Mice Smoking - adverse effects 吸烟 支气管上皮细胞 细胞凋亡 |
| Title | Relationship between Apoptosis and E-cadherin Expression in Bronchial Epithelium of Smoking Mouse |
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