Humoral immune response to hepatitis B vaccination: a laboratory practical for medical and bioscience undergraduates
From 1991 to 1997, laboratory exercises have been conducted using an enzyme-linked immunosorbent assay (ELISA) kit to determine the presence of antibodies to hepatitis B virus (HBV) surface antigen among a total of 690 medical and science undergraduates aged 18–25 years. Out of 431 students who rece...
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| Published in | Biochemical education Vol. 27; no. 1; p. 59 |
|---|---|
| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier Science
1999
|
| Online Access | Get full text |
| ISSN | 0307-4412 1879-1468 |
| DOI | 10.1016/S0307-4412(98)00222-2 |
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| Abstract | From 1991 to 1997, laboratory exercises have been conducted using an enzyme-linked immunosorbent assay (ELISA) kit to determine the presence of antibodies to hepatitis B virus (HBV) surface antigen among a total of 690 medical and science undergraduates aged 18–25 years. Out of 431 students who received at least the full course of three doses of HBV vaccine, 376 (87.2%) tested positive for HBV antibodies, demonstrating the immunogenicity of the current recombinant yeast-derived vaccines. However, 12.8% (
55
431
) of vaccinees remained seronegative, thereby indicating the need for serological testing to ascertain seroconversion following HBV vaccination. Only 66.7% (
18
27
) of subjects who received less than three doses of HBV vaccine developed HBV antibodies, thus emphasizing the necessity for the complete vaccination protocol to achieve an optimal antibody response. The vast majority of non-vaccinated students (91.1% or
205
225
) were seronegative, and advised HBV immunization. Particularly in regions such as Singapore where HBV infection is endemic, this practical study has good health education value, reinforces the principles and applications of the ELISA technique, and integrates certain key concepts of virology, immunology, molecular biology and medicine. |
|---|---|
| AbstractList | From 1991 to 1997, laboratory exercises have been conducted using an enzyme-linked immunosorbent assay (ELISA) kit to determine the presence of antibodies to hepatitis B virus (HBV) surface antigen among a total of 690 medical and science undergraduates aged 18–25 years. Out of 431 students who received at least the full course of three doses of HBV vaccine, 376 (87.2%) tested positive for HBV antibodies, demonstrating the immunogenicity of the current recombinant yeast-derived vaccines. However, 12.8% (
55
431
) of vaccinees remained seronegative, thereby indicating the need for serological testing to ascertain seroconversion following HBV vaccination. Only 66.7% (
18
27
) of subjects who received less than three doses of HBV vaccine developed HBV antibodies, thus emphasizing the necessity for the complete vaccination protocol to achieve an optimal antibody response. The vast majority of non-vaccinated students (91.1% or
205
225
) were seronegative, and advised HBV immunization. Particularly in regions such as Singapore where HBV infection is endemic, this practical study has good health education value, reinforces the principles and applications of the ELISA technique, and integrates certain key concepts of virology, immunology, molecular biology and medicine. |
| Author | Chan, S.H. Chow, Vincent T.K. |
| Author_xml | – sequence: 1 givenname: Vincent T.K. surname: Chow fullname: Chow, Vincent T.K. email: micctk@nus.edu.sg – sequence: 2 givenname: S.H. surname: Chan fullname: Chan, S.H. |
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| Cites_doi | 10.1016/S0163-4453(96)80019-3 10.4269/ajtmh.1997.56.76 10.1001/jama.1988.03410150079034 10.1128/JCM.18.3.535-538.1983 10.1002/jmv.1890210107 10.1093/ije/14.1.173 10.1016/S0140-6736(89)90057-3 10.1001/jama.1994.03510350069039 10.1016/0888-0786(95)95347-S 10.1016/0140-6736(91)90059-X 10.1093/oxfordjournals.bmb.a072405 10.1177/095646249000100108 10.1056/NEJM198607243150401 10.1016/S0140-6736(89)93009-2 10.5694/j.1326-5377.1991.tb112862.x 10.1016/0264-410X(95)00056-7 10.1001/jama.1989.03420160094029 10.1016/S0307-4412(98)00100-9 10.1016/0140-6736(91)91367-4 |
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Bull. doi: 10.1093/oxfordjournals.bmb.a072405 – volume: 1 start-page: 32 year: 1990 ident: 10.1016/S0307-4412(98)00222-2_BIB25 article-title: Effectiveness of intradermal hepatitis B immunization of hospital staff publication-title: Int. J. STD AIDS doi: 10.1177/095646249000100108 – volume: 315 start-page: 209 year: 1986 ident: 10.1016/S0307-4412(98)00222-2_BIB14 article-title: Long-term immunogenicity and efficacy of hepatitis B vaccine in homosexual men publication-title: New England J. Med. doi: 10.1056/NEJM198607243150401 – volume: 2 start-page: 847 year: 1989 ident: 10.1016/S0307-4412(98)00222-2_BIB15 article-title: Half-life of HBs antibody after hepatitis B vaccination: an aid to timing of booster vaccination publication-title: Lancet doi: 10.1016/S0140-6736(89)93009-2 – volume: 154 start-page: 70 year: 1991 ident: 10.1016/S0307-4412(98)00222-2_BIB6 article-title: Hepatitis B immunisation publication-title: Med. J. Aust. doi: 10.5694/j.1326-5377.1991.tb112862.x – volume: 13 start-page: 1240 year: 1995 ident: 10.1016/S0307-4412(98)00222-2_BIB22 article-title: Hepatitis B vaccination: universal vaccination of newborn babies and children at 12 years of age versus high risk groups. A comparison in the field publication-title: Vaccine doi: 10.1016/0264-410X(95)00056-7 – volume: 261 start-page: 2362 year: 1989 ident: 10.1016/S0307-4412(98)00222-2_BIB16 article-title: Duration of immunogenicity and efficacy of hepatitis B vaccine in a Yupik Eskimo population publication-title: J. Am. Med. Assoc. doi: 10.1001/jama.1989.03420160094029 – ident: 10.1016/S0307-4412(98)00222-2_BIB8 doi: 10.1016/S0307-4412(98)00100-9 – volume: 70 start-page: 233 year: 1992 ident: 10.1016/S0307-4412(98)00222-2_BIB19 article-title: Comparison of the immune response of four different dosages of a yeast-recombinant hepatitis B vaccine in Singapore children: a four-year follow-up study publication-title: Bull. World Health Org. – volume: 337 start-page: 747 year: 1991 ident: 10.1016/S0307-4412(98)00222-2_BIB21 article-title: Vaccination against hepatitis B and protection against chronic viral carriage in The Gambia publication-title: Lancet doi: 10.1016/0140-6736(91)91367-4 – volume: 22 start-page: 163 year: 1993 ident: 10.1016/S0307-4412(98)00222-2_BIB2 publication-title: Ann. Acad. Med. |
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