Peculiaritis of Apomictic plants development Pyrus communis х Chaenomeles japonica (Thunb.) Lindl. in vitro conditions

• Published in: Plant varieties studying and protection no. 2(19); pp. 46 - 49
• Main Authors: Джафарова, В. Є., Голишкін, Л. В., Долматов, Є. О., Ташматова, Л. В.
• Format: Journal Article
• Language: English
• Published: Ukrainian Institute for Plant Variety Examination 01.05.2013
• Subjects: apomictic plants; Chaenomeles japonica; cytokinins; micropropagation; pears; pollen; pollination; Pyrus communis; rhizogenesis; roots; somatic embryogenesis
• ISSN: 2518-1017; 2518-7457; 2518-7457
• DOI: 10.21498/2518-1017.2(19).2013.58547

The development features of four samples of apomictic plants during micropropagation are presented. These plants have been derived from embryos after pear pollination with the pollen of Chaenomeles japanica at 55 and 70 days of their development. Different responses of samples on the tested concentration of cytokinin (6BAP) and its combination with gibberell acid are shown. Tendency of increasing of the coefficient of the reproduction of separate numbers of apomictic plants has been noted under changing of BAP concentration from 1 mg/l to 2 mg/l or BAP combination with GA3. For the purpose of optimization of the stage of conglomeration forming (buds and shoots) BAP concentration 1 and 2 mg/l should be alternated in a passage on the background of GA3 1,5 mg/l. For the first time the origin of apomictic plant roots during rhizogenesis has been retraced and the anatomical structure of roots in conditions in vitro has been studied. Root formation in vitro occurs in internal tissues of a shoot. Roots of apomictic plants formed in vitro are primary as in plants developed in vivo.