apoptosis in cell turnover

Cell proliferation and programmed cell death play an important roles in the maintenance of tissue dynamics in both physiologic and pathologic status. Increased proliferation and/or decreased programmed cell death or apoptosis can result in “abnormal proliferation”. On the other hand, decreased proli...

Full description

Saved in:
Bibliographic Details
Published inJournal of the Japan Society of the Reticuloendothelial System Vol. 35; no. 5; pp. 223 - 228
Main Authors Nagura, Hiroshi, Kimura, Kenji, Imatani, Akira, Sasano, Hironobu
Format Journal Article
LanguageJapanese
Published The Japanese Society for Lymphoreticular Tissue Research 1995
Subjects
Online AccessGet full text
ISSN0386-9725
1883-6801
DOI10.3960/jslrt1961.35.223

Cover

Abstract Cell proliferation and programmed cell death play an important roles in the maintenance of tissue dynamics in both physiologic and pathologic status. Increased proliferation and/or decreased programmed cell death or apoptosis can result in “abnormal proliferation”. On the other hand, decreased proliferation and/or increased apoptosis may cause “loss of the cells”. Therefore, simultaneous analysis of both cell proliferation and apoptosis can provide important information on understanding physiology and pathology of various tissues. Recent advent in immunohistochemistry including ki67 immunostain with microwave irradiation for antigen retrieval and MIB-1 monoclonal antibody and development of 3'-OH nick end labeling technique or TUNEL method made it possible to assess cell proliferation and apoptosis in formalin-fixed and paraffin-embedded materials with reasonable accuracy. We applied these methods to human adrenal cortex and its disor-ders and gastric mucosa with or without atrophy or intestinal metaplasia in order to understand tissue dynamics. In the human adrenal glands, apoptosis appears to play important roles in the maintenance of organized cortical cell turnover, i.e., centripetal movement of the cortical cells or cell proliferation in the outer fasciculata and cell death in the zona glomerulosa and the zona reticularis, and in tumor cell dynamics in adrenocortical neoplasms. In non-neoplastic human gastric mucosa, marked increase of the number of the cells positive for both Ki67 and 3'-OH nick end labeling was observed in the gastric glands associated with incomplete intestinal metaplasia. In addition, marked mRNA hybridization signals of p53 was detected in the cells undergoing apoptosis in these glands. Gastric glands associated with incomplete intestinal metaplasia are therefore characterized by increased cells turnover and possibly p53 induced apoptosis.
AbstractList Cell proliferation and programmed cell death play an important roles in the maintenance of tissue dynamics in both physiologic and pathologic status. Increased proliferation and/or decreased programmed cell death or apoptosis can result in “abnormal proliferation”. On the other hand, decreased proliferation and/or increased apoptosis may cause “loss of the cells”. Therefore, simultaneous analysis of both cell proliferation and apoptosis can provide important information on understanding physiology and pathology of various tissues. Recent advent in immunohistochemistry including ki67 immunostain with microwave irradiation for antigen retrieval and MIB-1 monoclonal antibody and development of 3'-OH nick end labeling technique or TUNEL method made it possible to assess cell proliferation and apoptosis in formalin-fixed and paraffin-embedded materials with reasonable accuracy. We applied these methods to human adrenal cortex and its disor-ders and gastric mucosa with or without atrophy or intestinal metaplasia in order to understand tissue dynamics. In the human adrenal glands, apoptosis appears to play important roles in the maintenance of organized cortical cell turnover, i.e., centripetal movement of the cortical cells or cell proliferation in the outer fasciculata and cell death in the zona glomerulosa and the zona reticularis, and in tumor cell dynamics in adrenocortical neoplasms. In non-neoplastic human gastric mucosa, marked increase of the number of the cells positive for both Ki67 and 3'-OH nick end labeling was observed in the gastric glands associated with incomplete intestinal metaplasia. In addition, marked mRNA hybridization signals of p53 was detected in the cells undergoing apoptosis in these glands. Gastric glands associated with incomplete intestinal metaplasia are therefore characterized by increased cells turnover and possibly p53 induced apoptosis.
Author Nagura, Hiroshi
Kimura, Kenji
Imatani, Akira
Sasano, Hironobu
Author_xml – sequence: 1
  fullname: Nagura, Hiroshi
  organization: Second Department of Pathology, Tohoku University School of Medicine
– sequence: 1
  fullname: Kimura, Kenji
  organization: Third Department of Internal Medicine, Tohoku University School of Medicine
– sequence: 1
  fullname: Imatani, Akira
  organization: Third Department of Internal Medicine, Tohoku University School of Medicine
– sequence: 1
  fullname: Sasano, Hironobu
  organization: Second Department of Pathology, Tohoku University School of Medicine
BookMark eNpVz01LxDAQgOEgK1jXvYun_oHWSaZJk6Ms6wcseNFzSNJEu9S2JFXw31upFLzMHIZn4L0km37oPSHXFEpUAm5PqYsTVYKWyEvG8IxkVEoshAS6IRmgFIWqGb8gu5RaC8AAUHHMyI0Zh3EaUpvyts-d77p8-oz98OXjFTkPpkt-97e35PX-8LJ_LI7PD0_7u2PhGDAsKGWAVFHlhOCqdg0qG4KopbDWBoAKbCVRIDJFLcXgG7SukqJqwDghBW4JLH9dHFKKPugxth8mfmsK-jdPr3kauZ7zZnJYyClN5s2vwMSpdZ3_D_gyZrfe3buJ2vf4A78pXKg
ContentType Journal Article
Copyright by The Japanese Society for Lymphoreticular Tissue Research
Copyright_xml – notice: by The Japanese Society for Lymphoreticular Tissue Research
DBID AAYXX
CITATION
DOI 10.3960/jslrt1961.35.223
DatabaseName CrossRef
DatabaseTitle CrossRef
DatabaseTitleList
DeliveryMethod fulltext_linktorsrc
EISSN 1883-6801
EndPage 228
ExternalDocumentID 10_3960_jslrt1961_35_223
article_jslrt1961_35_5_35_5_223_article_char_en
GroupedDBID ALMA_UNASSIGNED_HOLDINGS
JSF
KQ8
RJT
AAYXX
CITATION
ID FETCH-LOGICAL-c2023-112031919c66597cd39bff6786bbbf0040b483633291b13fed3bc4864d0ac6863
ISSN 0386-9725
IngestDate Tue Jul 01 00:59:38 EDT 2025
Wed Sep 03 06:15:57 EDT 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed false
IsScholarly false
Issue 5
Language Japanese
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c2023-112031919c66597cd39bff6786bbbf0040b483633291b13fed3bc4864d0ac6863
OpenAccessLink https://www.jstage.jst.go.jp/article/jslrt1961/35/5/35_5_223/_article/-char/en
PageCount 6
ParticipantIDs crossref_primary_10_3960_jslrt1961_35_223
jstage_primary_article_jslrt1961_35_5_35_5_223_article_char_en
ProviderPackageCode CITATION
AAYXX
PublicationCentury 1900
PublicationDate 1995
1995-00-00
PublicationDateYYYYMMDD 1995-01-01
PublicationDate_xml – year: 1995
  text: 1995
PublicationDecade 1990
PublicationTitle Journal of the Japan Society of the Reticuloendothelial System
PublicationTitleAlternate J.Jap.Soc.R.E.S
PublicationYear 1995
Publisher The Japanese Society for Lymphoreticular Tissue Research
Publisher_xml – name: The Japanese Society for Lymphoreticular Tissue Research
References 1) Thompson CB: Apoptosis in the pathogenesis and treatment of disease Science. 267: 1456-1462 1995
3) Sasano H, Imatani A, Shizawa S, Suzuki T, Nagura H: Cell proliferation and apoptosis in normal and pathologic human adrenal. Modern Pathol 8: 11-17 1995
2) Gavrieli Y, Sherman Y, Ben-Sasson, SA: Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 119: 493-501 1992
4) Fritsche M, Haessler C, Brandner G: Induction of nuclear accumulation of the tumor suppressor protein p 53 by DNA damaging agents. Oncogene 8: 307-318 1993
5) Sasano H, Goukon Y, Nishihira T, Nagura H, In situ hybridization of p 53 tumur suppressor gene in human esophageal carcinoma. Am J Pathol 141: 545-550 1992
References_xml – reference: 1) Thompson CB: Apoptosis in the pathogenesis and treatment of disease Science. 267: 1456-1462 1995
– reference: 3) Sasano H, Imatani A, Shizawa S, Suzuki T, Nagura H: Cell proliferation and apoptosis in normal and pathologic human adrenal. Modern Pathol 8: 11-17 1995
– reference: 5) Sasano H, Goukon Y, Nishihira T, Nagura H, In situ hybridization of p 53 tumur suppressor gene in human esophageal carcinoma. Am J Pathol 141: 545-550 1992
– reference: 2) Gavrieli Y, Sherman Y, Ben-Sasson, SA: Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 119: 493-501 1992
– reference: 4) Fritsche M, Haessler C, Brandner G: Induction of nuclear accumulation of the tumor suppressor protein p 53 by DNA damaging agents. Oncogene 8: 307-318 1993
SSID ssib002003953
ssib002670958
ssib005879743
ssib058493860
ssib002671034
ssj0003236747
Score 1.304444
Snippet Cell proliferation and programmed cell death play an important roles in the maintenance of tissue dynamics in both physiologic and pathologic status. Increased...
SourceID crossref
jstage
SourceType Index Database
Publisher
StartPage 223
SubjectTerms adrenal
apoptosis
cell turnover
proliferation
stomach
Title apoptosis in cell turnover
URI https://www.jstage.jst.go.jp/article/jslrt1961/35/5/35_5_223/_article/-char/en
Volume 35
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
ispartofPNX Journal of the Japan Society of the Reticuloendothelial System, 1995, Vol.35(5), pp.223-228
journalDatabaseRights – providerCode: PRVAFT
  databaseName: Colorado Digital library
  customDbUrl:
  eissn: 1883-6801
  dateEnd: 19961231
  omitProxy: true
  ssIdentifier: ssj0003236747
  issn: 0386-9725
  databaseCode: KQ8
  dateStart: 19610101
  isFulltext: true
  titleUrlDefault: http://grweb.coalliance.org/oadl/oadl.html
  providerName: Colorado Alliance of Research Libraries
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Pb9MwFLZg48AFbRqIDTblsB1QlZLEseNcJlX7VZBWCWmTdovs1IE0I6269MJfz3ux63kVSIxLlKa2E-e9PH_P9vceIcexppwLhnFbEx6mSSRDWaVJiKROIXUqo54hdz3h49v06x27e8zZ2rNLOjUsf_2RV_I_UoVrIFdkyT5Dsq5RuADnIF84goTh-E8yHi3mi26OIUVwNznOwsEA0uKmTB9zTuofIOLrkzN2Is5bWTe6HlzJppH1oMHky7WbZZEPss_EPRgj-W2uVo8r9D9XS2l5PDNX4QvAXZMTajBq6qWz8BP53RbHhtZ3mFqyHfOMDxU8zDNDSh5qYxyFoCEXdvLBWk_KPC1hvik0PGI7qiaGAr5psCk4UGiwH-6XHdiCeEjZ0FX0Y2NvjFluJyH4MNhG4VooKCughZdkO8kAe-Ay_TfPoUIusg9QMHydty4Mv-PIC5jHRAYOlysPaC2HFxO5WTyKAfD6LHbuhZm1cHyoz5vdeoJ9Xs0A_q-3DvZo5maHvLFuSDAyOrVLXszkHjlw-hTUbYD6FKz16S25vby4ORuHNndGWCYAw0KA0chPi_OSc_AZyynNVVUBMuFKqQpNt0oF5ZQmeaxiWukpVWUqeDqNZMkFp-_IVjtv9XsSAKAEjKgrXsosVbESSsK5zriSCc1yvk8-rXtVLEyIlOJvYtknp6bbrqT9eJ6WZOYAFdz_SEKEb_7gGTf7QF6bAAQ4YfaRbHXLlT4ECNmpo14rfgOM4mV5
linkProvider Colorado Alliance of Research Libraries
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Apoptosis+in+cell+turnover&rft.jtitle=Nihon+M%C5%8Dnaikei+Gakkai+kaishi&rft.au=Sasano%2C+Hironobu&rft.au=Kimura%2C+Kenji&rft.au=Imatani%2C+Akira&rft.au=Nagura%2C+Hiroshi&rft.date=1995&rft.issn=0386-9725&rft.eissn=1883-6801&rft.volume=35&rft.issue=5&rft.spage=223&rft.epage=228&rft_id=info:doi/10.3960%2Fjslrt1961.35.223&rft.externalDBID=n%2Fa&rft.externalDocID=10_3960_jslrt1961_35_223
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0386-9725&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0386-9725&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0386-9725&client=summon