Polyethylene glycol embedding for immunofluorescence microscopy of plant tissues

Polyethylene glycol embedding is an useful method for immunofluorescence microscopy of plant tissues. The dehydration, infiltration and embedding of materials into the polyethylene glycol is easier than the procedures in the usual paraffin method. And the sectioning is easier than cryosectioning. Se...

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Published inPLANT MORPHOLOGY Vol. 3; no. 1; pp. 31 - 35
Main Author Uehara, Koichi
Format Journal Article
LanguageEnglish
Published The Japanese Society of Plant Morphology 1991
Subjects
elater
Equisetum
immunofluorescence microscopy
microtubules
Polyethylene glycol 1540
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ISSN0918-9726
1884-4154
1884-4154
DOI10.5685/plmorphol.3.31

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Abstract Polyethylene glycol embedding is an useful method for immunofluorescence microscopy of plant tissues. The dehydration, infiltration and embedding of materials into the polyethylene glycol is easier than the procedures in the usual paraffin method. And the sectioning is easier than cryosectioning. Serial sections of 5-10μm thickness are obtained with a rotary microtome for various tissues of higher plants. This method is useful for immunofluorescence microscopy of microtubules. Microtubule arrangements in elate formation of Equisetum spore are observed by this method.
AbstractList Polyethylene glycol embedding is an useful method for immunofluorescence microscopy of plant tissues. The dehydration, infiltration and embedding of materials into the polyethylene glycol is easier than the procedures in the usual paraffin method. And the sectioning is easier than cryosectioning. Serial sections of 5-10μm thickness are obtained with a rotary microtome for various tissues of higher plants. This method is useful for immunofluorescence microscopy of microtubules. Microtubule arrangements in elate formation of Equisetum spore are observed by this method.
Author Uehara, Koichi
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References Hogetsu, T. 1989. The arrangement of microtubules in leaves of monocotyledonous and dicotyledonous plants. Canad. J. Bot. 67: 3506-3512.
Sitte, P. 1963. Bau und Bewegung der Sporen-Hapteren bei Equisetum arvense L. Ber. Naturwiss. -Med. Vereins Innsbruck 53: 193-207.
Uehara, K., and S. Kurita. 1989. An ultrastructural study of spore wall morphogenesis in Equisetum arvense. Amer. J. Bot. 76: 939-951.
Osborn, M., and K. Weber. 1982. Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: tubulin-containing structures. in L. Wilson ed., Methods in Cell Biology 24: 97-132 Academic Press.
References_xml – reference: Osborn, M., and K. Weber. 1982. Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: tubulin-containing structures. in L. Wilson ed., Methods in Cell Biology 24: 97-132 Academic Press.
– reference: Sitte, P. 1963. Bau und Bewegung der Sporen-Hapteren bei Equisetum arvense L. Ber. Naturwiss. -Med. Vereins Innsbruck 53: 193-207.
– reference: Uehara, K., and S. Kurita. 1989. An ultrastructural study of spore wall morphogenesis in Equisetum arvense. Amer. J. Bot. 76: 939-951.
– reference: Hogetsu, T. 1989. The arrangement of microtubules in leaves of monocotyledonous and dicotyledonous plants. Canad. J. Bot. 67: 3506-3512.
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SubjectTerms elater
Equisetum
immunofluorescence microscopy
microtubules
Polyethylene glycol 1540
Title Polyethylene glycol embedding for immunofluorescence microscopy of plant tissues
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