Effect of Non-resorbable Membrane on Bone Formation after Implant of Apatite Blocks on Bone

• Published in: Nihon Hotetsu Shika Gakkai Zasshi Vol. 46; no. 5; pp. 712 - 721
• Main Authors: Kawasaki, Takao, Ohori, Kotoha, Kohgo, Takao, Iizuka, Tadashi, Yokoyama, Atsuro
• Format: Journal Article
• Language: Japanese
• Published: Japan Prosthodontic Society 2002
• Subjects: expanded polytetrafluoroethylene membrane; guided bone regeneration method; histomorphometrics; porous hydroxyapatite block; tartrate-resistant acid phosphatase
• ISSN: 0389-5386; 1883-177X; 1883-177X
• DOI: 10.2186/jjps.46.712

Purpose: The purpose of this study was to evaluate osteogenesis, histopathologically and histomorphometrically, around porous hydroxyapatite (HAP) blockson bone with or without expanded polytetrafluoroethylene (e-PTFE) membrane, as an application of the guided bone regeneration (GBR) method. Materials and Methods: In the experimental group, HAP blocks were implanted on the parietal bone of rats and covered by e-PTFE membranes. In the control group, only HAP blocks were implanted between the parietal bone and periosteum. Tissue reaction and osteogenesis around HAP blocks were evaluated histopathologically and histomorphometrically. Some specimens were stained with tartrate-resistant acid phosphatase (TRAP). Results: New bone had attached directly to HAP at 2 weeksafter implantation in both groups, and after that, new bone grew up. Newly formed bone was recognized in the upper area of the pore of HAP blocks in the experimental group, in comparison with the control group. The amount of newly formed bone in the experimental group was significantly more compared with the control group at 4 weeks, while the significance was not recognized at 8 weeks. The numberof TRAP-positive multinucleated giant cells increased until 4 weeks, and decreased at 8 weeks, in both groups. The number of TRAP-positive multinucleated giant cells in the experimental group was significantly more than in the control group, and it was suggested that bone remodeling was active. Conclusions: These results suggest that the utilization of e-PTFE membrane as an application of the GBR method is effective for osteogenesis in HAP grafts on bone.