Differential expression of βig-h3 in human hepatoma cell lines

• Published in: Journal of Medical Colleges of PLA Vol. 22; no. 1; pp. 8 - 11
• Main Author: 郭卫平 周宏伟 蒋建利 张洪新
• Format: Journal Article
• Language: English
• Published: Department of Interventional Radiation,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038,China%Cell Engineering Research Centre,Fourth Military Medical University,Xi'an 710033,China 01.02.2007
• Subjects: βig-h3; 差异表达; 肝细胞瘤细胞系; 肿瘤转移; adhesion; HAb18G/CD147; hepatoma; βig-h3
• ISSN: 1000-1948
• DOI: 10.1016/S1000-1948(07)60002-1

Objective：To observe the differential expression of TGF-β-induced gene human clone 3 （βig- h3） in human hepatoma cell lines. Methods：Human hepatoma cells HHCC, 7721, T7721 constructed by stably transfectiug HAb18G/CD147 cDNA into 7721 and normal human liver cell QZG were cultured as previously. RT-PCR and western blot were used to investigate the differential expression of βig-h3 in human hepatoma cell lines and normal liver cell. Results ：The results of RT-PCR suggested that the expression of βig-h3 mRNA in human hepatoma cells was higher than that in normal human liver cell QZG（P〈0.01）, and its expression level in human hepatoma cells in turn was HHCC〉T7721〉7721. Moreover, the similar results of βig-h3 protein expression were testified by western blot. Conclusion：This study demonstrates that the expression of βig-h3 in hepatoma cell lines is higher than that in normal liver cell QZG, which provides a sound basis for exploring the function of βig-h3 in processes of adhesion and metastasis of human hepatoma cells.