Designing and Cloning of the Gene Sequence Encoding Silk Fibroin Amorphous Domain

• Published in: 东华大学学报（英文版） Vol. 29; no. 6; pp. 489 - 492
• Main Author: 黄海燕 田智芳 裔洪根 杨云星 王建南
• Format: Journal Article
• Language: English
• Published: College of Textile and Clothing Engineering, Soochow University, Suzhou 215021, China%College of Textile and Clothing Engineering, Soochow University, Suzhou 215021, China 2012; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215021, China
• Subjects: amorphous domain; gene cloning; DNA electrophoresis; Escherichia coli; silk fibroin
• ISSN: 1672-5220

To provide materials used in investigating the relationship between amino acid compositions of silk-like protein, structure, and functions, especially the biological functions, the motif genes encoding the silk fibroin amorphous domain, SGFGPVANGGSGEASSESDFGSSGFGPVANASSGEASSESDFAG （F） were designed and extended using a ＂head-to-tail＂ construction strategy. The designed genes were cloned into PSLFA1180FA and multimerized to form structures containing a two-timer, a four- timer, an eight-timer, and a twelve-timer. All the resulting plasmids were digested using the restriction enzyme BamI-II and the double- enzymes BgllL/HindHI. Restriction enzyme analysis and DNA sequencing revealed the motif was successfully cloned into PSLFAll80FA and mnitimerized to form a twelve-timer without eene deletion or mutation.