DIFFERENTIAL EXPRESSION OF GENES INVOLVED IN METABOLISM BETWEEN TUMORIGENITIC HUMAN LEUKEMIA CELL LINES K562 AND K562－n

• Published in: Chinese journal of cancer research Vol. 15; no. 2; pp. 102 - 106
• Main Author: 吕书晴 许小平 夏放 居小萍 李瑶 应康 毛裕民
• Format: Journal Article
• Language: English
• Published: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433%Institute of Genetics, Fudan University, Shanghai 200433 01.06.2003
• Subjects: K562; K562－n; 基因表达; 白血病; 致肿瘤性; Leukemic cell; Tumorigenicity; Gene expression; Metabolism
• ISSN: 1000-9604; 1993-0631
• DOI: 10.1007/BF02974910

Objective: To study the molecular mechanism of different tumorigenicity in nude mice of human leukemia cell lines K562-n and K562. Methods: To analyze the genes differently expressed between K562 and K562-n cells by using cDNA microarray technique. Results: Among the 12800 genes detected, some genes involved in material metabolism and material transport were differently expressed between K562-n and K562 cells. These genes include homo sapiens placenta-specific ATP-binding cassette transporter gene, dihydrodiol dehydrogenase gene,hepatic dihydrodiol dehydrogenase gene, NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase,lysophosphatidic acid acyltransferase, alpha gene,argininosuccinate lyase gene, mitochondrial isocitrtated ehydrogenase, adhesion protein SQM1 gene,dimethylarginine dimethylamino-hydrolase gene, M1 subunit of ribonucleotide reductase and farnesyl pyrophosphate synthetase gene. Conclusion: The high tumorigenicity of K562-n cells is related to the different expression of some genes concerned with cell metabolism and material transpoert.