Measurement of spontaneous platelet aggregation in patients with acute cerebral infarction using laser-scattered light aggregometry

Quantitative analysis of platelet aggregation is difficult, and the conditions of platelet aggregation in patients with acute cerebral infarction have not previously been examined in sufficient detail. We quantitated the spontaneous platelet aggregation (SPA) in patients with acute cerebral infarcti...

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Published inJapanese Journal of Stroke Vol. 26; no. 2; pp. 364 - 370
Main Authors Sugihara, Hiroshi, Yazaki, Naoko, Toshima, Hiroko, Yamada, Koji, Hirayama, Toshikazu, Takahashi, Youichi
Format Journal Article
LanguageJapanese
Published The Japan Stroke Society 2004
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ISSN0912-0726
1883-1923
DOI10.3995/jstroke.26.364

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Abstract Quantitative analysis of platelet aggregation is difficult, and the conditions of platelet aggregation in patients with acute cerebral infarction have not previously been examined in sufficient detail. We quantitated the spontaneous platelet aggregation (SPA) in patients with acute cerebral infarction using laser-scattered light aggregometry. The subjects comprised 98 patients with acute cerebral infarction. The subtype diagnoses included atherothrombotic infarction (ATI ; n = 45), lacunar infarction (LI ; n = 21) and cardioembolic in. farction (CE; n=32).The SPA was evaluated on days 1, 3, 7, 14 and 28 after the onset of cerebral infarction.The serum lipid, plasma glucose and diastolic blood pressure levels were relevant to the SPA after the onset of cerebral infarction. The maximum SPA was significantly increased in the patients with ATI and CE as compared to those with LI. No significant changes in SPA were recognized during the acute stage of cerebral infarction in cases of ATI or LI. The SPA on day 3 was significantly increased as compared to that on day 14 in CE. These findings suggest that activation of SPA differs among patients with different subtypes of cerebral infarction, and the SPA represents a useful marker of platelet activation.
AbstractList Quantitative analysis of platelet aggregation is difficult, and the conditions of platelet aggregation in patients with acute cerebral infarction have not previously been examined in sufficient detail. We quantitated the spontaneous platelet aggregation (SPA) in patients with acute cerebral infarction using laser-scattered light aggregometry. The subjects comprised 98 patients with acute cerebral infarction. The subtype diagnoses included atherothrombotic infarction (ATI ; n = 45), lacunar infarction (LI ; n = 21) and cardioembolic in. farction (CE; n=32).The SPA was evaluated on days 1, 3, 7, 14 and 28 after the onset of cerebral infarction.The serum lipid, plasma glucose and diastolic blood pressure levels were relevant to the SPA after the onset of cerebral infarction. The maximum SPA was significantly increased in the patients with ATI and CE as compared to those with LI. No significant changes in SPA were recognized during the acute stage of cerebral infarction in cases of ATI or LI. The SPA on day 3 was significantly increased as compared to that on day 14 in CE. These findings suggest that activation of SPA differs among patients with different subtypes of cerebral infarction, and the SPA represents a useful marker of platelet activation.
Author Yamada, Koji
Takahashi, Youichi
Sugihara, Hiroshi
Toshima, Hiroko
Yazaki, Naoko
Hirayama, Toshikazu
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  fullname: Hirayama, Toshikazu
  organization: Department of Neurology, St. Marianna University School of Medicine
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  fullname: Takahashi, Youichi
  organization: Department of Neurology, St. Marianna University School of Medicine
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References 11) Whisnant JP, Basford JR, Bernstein EF, et al : Special report from the National Institute of Neurological Disorders and Stroke : Classification of cerebrovascular disease III. Stroke 21 : 637—676, 1990
26) 内山真一郎,山崎昌子,岩田誠ら:心原性脳塞栓症における心内血栓の画像診断と血小板・凝固・線溶活性化.臨床神経 36:429―435,1996
3) 清水美衣,山本正博,安藤泰彦ら:急性期および慢性期脳梗塞患者における活性化血小板の検出.脳卒中 24:234―239,2002
4) Yamazaki M, Uchiyama S, Iwata M : Measurement of platelet fibrinogen binding and p-selectin expression by flow cytometry in patients with cerebral infarction. Thromb Res 104 : 197—205, 2001
25) 内山真一郎,望月昌子,鄭秀明ら:虚血性脳卒中におけるindium-111tropolone血小板標識法による抗血栓療法の評価.神経内科治療 8:57―62,1991
22) 高野健太郎:脳梗塞急性期の凝固・線溶動態. Prog. Med. 17:2964―2968,1997
10) 佐藤金夫,尾崎由基男,矢富裕ら:散乱光を用いた粒子計測法による血小板凝集能の測定.血栓止血誌 8:55―61,1997
18) Musumeci V, Carloni L, Marchetti P, et al : Platelet aggregation in native blood exposed to a shifting shear rate field. Thromb Res 21: 45—51,1981
19) Ikeda Y, Kawano K, Handa M, et al : Determination of intracellular calcium ions during shear-induced platelet aggregation. Acta Haematol Jpn 52:1549—1554,1989
16) Michelson AD : Flow cytometry : a clinical test of platelet function. Blood 87: 4925—4936,1996
9) Ozaki Y, Satoh K, Yatomi Y, et al : Detection of platelet aggregates with a particle counting method using light scattering. Anal Biochem 218: 284—294,1994
5) Kozuka K, Kohriyama T, Nomura E, et al : Endothelial markers and adhesion molecules in acute ischemic stroke-sequential change and differences in stroke subtype. Atherosclerosis 161 161—168, 2002
2) Shah AB, Beamer N, Coull BM: Enhanced in vivo platelet activation in subtypes of ischemic stroke. Stroke 16: 643—647.1985
14) 内山真一郎,山崎昌子:脳卒中における血小板機能,凝固・線溶能.脳卒中臨床の基礎.脳卒中.Medicina 32:2144―2148,1995
15) 内山真一郎,會根玲子,柴垣泰郎ら:脳虚血における血小板凝集のmultipathwayと血小板放出反応.臨床神経28:1065―1071,1988
20) Uchiyama S, Yamazaki M, Maruyama S, et al : Shear-induced platelet aggregation in cerebral ischemia. Stroke 25: 1547—1551, 1994
7) Marquardt L, Ruf A, Mansmann U, et al : Course of platelet activation markers after ischemic stroke. Stroke 33: 2570—2574, 2002
12) Astrup J. Siesjö BK, Symon L : Thresholds in cerebral ischemia—The ischemic penumbra. Stroke 12: 723—725,1981
8) Cherian P, Hankey GJ, Eikelboom JW, et al : Endothelial and platelet activation in acute ischemic stroke and its etiological subtypes. Stroke 34 2132—2137.2003
6) Cha JK, Jeong MH, Lee KM, et al : Changes in platelet P-selectin and in plasma C-reactive protein in acute atherosclerotic ischemic stroke treated with a loading dose of clopidogrel. J Thromb Thrombolysis 14: 145—150,2002
24) 棚橋紀夫:脳梗塞A.急性期の病態e.血小板活性化と凝固・血液レオロジー異常。神経内科58(Suppl.3):138-146,2003
13) 内山真一郎:PAFと脳虚血.蔵本淳,青木延雄,池田康夫ら(編):抗血小板療法の基礎と臨床,京都,金芳堂,1993,pp23―36
17) Goldsmith HL, Marlow JC, Yu SK : The effect of oscillatory flow on the release reaction and aggregation of human platelets. Microvasc Res 11 335—359,1976
23) Landi G, Barbarotto R, Morabito A, et al : Prognostic significance of fibrinopeptide A in survivors of cerebral infarction. Stroke 21 : 424—427, 1990
1) 内山真一郎,山崎昌子,岩田誠:脳虚血と血小板活性化.神経進歩 40:666―678,1996
21) Uchiyama S, Yamazaki M, Maruyama S, et al : Shear-induced platelet aggregation and its inhibition by antiplatelet agents in cerebral ischemia. Clin Hemorheol 13: 623—636.1993
References_xml – reference: 4) Yamazaki M, Uchiyama S, Iwata M : Measurement of platelet fibrinogen binding and p-selectin expression by flow cytometry in patients with cerebral infarction. Thromb Res 104 : 197—205, 2001
– reference: 11) Whisnant JP, Basford JR, Bernstein EF, et al : Special report from the National Institute of Neurological Disorders and Stroke : Classification of cerebrovascular disease III. Stroke 21 : 637—676, 1990
– reference: 21) Uchiyama S, Yamazaki M, Maruyama S, et al : Shear-induced platelet aggregation and its inhibition by antiplatelet agents in cerebral ischemia. Clin Hemorheol 13: 623—636.1993
– reference: 14) 内山真一郎,山崎昌子:脳卒中における血小板機能,凝固・線溶能.脳卒中臨床の基礎.脳卒中.Medicina 32:2144―2148,1995
– reference: 19) Ikeda Y, Kawano K, Handa M, et al : Determination of intracellular calcium ions during shear-induced platelet aggregation. Acta Haematol Jpn 52:1549—1554,1989
– reference: 20) Uchiyama S, Yamazaki M, Maruyama S, et al : Shear-induced platelet aggregation in cerebral ischemia. Stroke 25: 1547—1551, 1994
– reference: 18) Musumeci V, Carloni L, Marchetti P, et al : Platelet aggregation in native blood exposed to a shifting shear rate field. Thromb Res 21: 45—51,1981
– reference: 12) Astrup J. Siesjö BK, Symon L : Thresholds in cerebral ischemia—The ischemic penumbra. Stroke 12: 723—725,1981
– reference: 17) Goldsmith HL, Marlow JC, Yu SK : The effect of oscillatory flow on the release reaction and aggregation of human platelets. Microvasc Res 11 335—359,1976
– reference: 22) 高野健太郎:脳梗塞急性期の凝固・線溶動態. Prog. Med. 17:2964―2968,1997
– reference: 1) 内山真一郎,山崎昌子,岩田誠:脳虚血と血小板活性化.神経進歩 40:666―678,1996
– reference: 8) Cherian P, Hankey GJ, Eikelboom JW, et al : Endothelial and platelet activation in acute ischemic stroke and its etiological subtypes. Stroke 34 2132—2137.2003
– reference: 2) Shah AB, Beamer N, Coull BM: Enhanced in vivo platelet activation in subtypes of ischemic stroke. Stroke 16: 643—647.1985
– reference: 13) 内山真一郎:PAFと脳虚血.蔵本淳,青木延雄,池田康夫ら(編):抗血小板療法の基礎と臨床,京都,金芳堂,1993,pp23―36
– reference: 25) 内山真一郎,望月昌子,鄭秀明ら:虚血性脳卒中におけるindium-111tropolone血小板標識法による抗血栓療法の評価.神経内科治療 8:57―62,1991
– reference: 6) Cha JK, Jeong MH, Lee KM, et al : Changes in platelet P-selectin and in plasma C-reactive protein in acute atherosclerotic ischemic stroke treated with a loading dose of clopidogrel. J Thromb Thrombolysis 14: 145—150,2002
– reference: 26) 内山真一郎,山崎昌子,岩田誠ら:心原性脳塞栓症における心内血栓の画像診断と血小板・凝固・線溶活性化.臨床神経 36:429―435,1996
– reference: 16) Michelson AD : Flow cytometry : a clinical test of platelet function. Blood 87: 4925—4936,1996
– reference: 15) 内山真一郎,會根玲子,柴垣泰郎ら:脳虚血における血小板凝集のmultipathwayと血小板放出反応.臨床神経28:1065―1071,1988
– reference: 3) 清水美衣,山本正博,安藤泰彦ら:急性期および慢性期脳梗塞患者における活性化血小板の検出.脳卒中 24:234―239,2002
– reference: 5) Kozuka K, Kohriyama T, Nomura E, et al : Endothelial markers and adhesion molecules in acute ischemic stroke-sequential change and differences in stroke subtype. Atherosclerosis 161 161—168, 2002
– reference: 7) Marquardt L, Ruf A, Mansmann U, et al : Course of platelet activation markers after ischemic stroke. Stroke 33: 2570—2574, 2002
– reference: 9) Ozaki Y, Satoh K, Yatomi Y, et al : Detection of platelet aggregates with a particle counting method using light scattering. Anal Biochem 218: 284—294,1994
– reference: 24) 棚橋紀夫:脳梗塞A.急性期の病態e.血小板活性化と凝固・血液レオロジー異常。神経内科58(Suppl.3):138-146,2003
– reference: 23) Landi G, Barbarotto R, Morabito A, et al : Prognostic significance of fibrinopeptide A in survivors of cerebral infarction. Stroke 21 : 424—427, 1990
– reference: 10) 佐藤金夫,尾崎由基男,矢富裕ら:散乱光を用いた粒子計測法による血小板凝集能の測定.血栓止血誌 8:55―61,1997
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SubjectTerms acute cerebral infarction
spontaneous platelet aggregation
Title Measurement of spontaneous platelet aggregation in patients with acute cerebral infarction using laser-scattered light aggregometry
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