The role of interleukin-7 in Porphyromonas gingivalis - stimulated vascular calcification

Periodontal disease has been implicated in the progression of various systemic diseases, including chronic kidney disease (CKD). Recent evidence suggests that infection of Porphyromonas gingivalis , a major periodontal pathogen, may also contribute to vascular calcification in patients with CKD. In...

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Bibliographic Details
Published inInternational journal of oral biology Vol. 49; no. 4; pp. 110 - 117
Main Authors Park, Hyun-Joo, Kim, Mi-Kyoung, Kim, Yeon, Bae, Soo-Kyung, Kim, Hyung Joon, Bae, Moon-Kyoung
Format Journal Article
LanguageEnglish
Published 대한구강생물학회 31.12.2024
Subjects
치의학
Porphyromonas gingivalis
Vascular calcification
Interleukin-7
Chronic kidney diseases
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ISSN1226-7155
2287-6618
DOI10.11620/IJOB.2024.49.4.110

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Table of Contents:
  • Introduction Materials and Methods 1. Bacterial strain and growth conditions 2. Induction of CKD in the mice model 3. Antibody array assay 4. Data acquisition and analysis 5. Enzyme-linked immunosorbent assay 6. Cell culture and P. gingivalis infection 7. Gene knockdown by small interfering RNA 8. Induction and quantification of calcification 9. Alizarin red S staining 10. Quantitative real-time reversetranscription-polymerase chain reaction 11. Western blot analysis 12. Statistical analysis Results 1. Oral administration of P. gingivalis alters serumprotein expression profiles in a CKD mouse model 2. Expression of IL-7 in P. gingivalis -infected vascularcalcification of VSMCs 3. Validation of IL-7 knockdown efficiency in VSMCs 4. Effect of IL-7 silencing on P. gingivalis -stimulatedVSMC calcification and osteogenic markerexpression Discussion Funding Conflicts of Interest References