INVOLVEMENT OF HLA-DRB111 AND HLA-DRB112 ALLELES IN THE OCCURRENCE OF DENGUE FEVER IN BURKINA FASO

• Published in: Journal of Experimental and Molecular Biology
• Main Authors: TRAORE, Lassina, KOUTA, Olawoumi Fabrice, TAPSOBA, Aziz Sidi Aristide, OUATTARA, Abdoul Karim, KANFON, Richard, YOUGBARE, Maïmounatou Rufaïda, TASSEMBEDO, Fadilatou, SAVADOGO, Mousso, SANON, Nafissatou, BELLO, Shoukrat Ohuwa Toyin, BAYALA, Bagora, DABIRE, Amana METUOR, YONLI, Albert Theophane, DJIGMA, Florencia Wendkuuni, SIMPORE, Jacques
• Format: Journal Article
• Language: English
• Published: "Alexandru Ioan Cuza" University of Iași 17.12.2024
• Subjects: Burkina Faso; Dengue; HLA -DRB112; HLA-DRB111; risk factors
• ISSN: 2601-6974; 2601-6974
• DOI: 10.47743/jemb-2025-195

Introduction: Dengue has become the world's most common arbovirosis. In some individuals, genetic factors can increase the risk of developing severe dengue fever. Human leukocyte antigen (HLA) genes are one of the most extensively studied gene groups in human disease. The present study investigated HLA DRB1*11 and HLA DRB1*12 polymorphisms in dengue cases and their susceptibilities in the development of dengue in a population in Ouagadougou, Burkina Faso. Methodology: This was a case-control study involving 56 patients with clinically and biologically confirmed dengue fever and 65 others who had never been in contact with DENV, for a total of 121 individuals. A blood sample was taken from each study participant. After extraction of genomic DNA using the salting-out technique, characterisation of carriage of the HLA-DRB1*11 and 1*12 alleles was carried out using multiplex polymerase chain reaction (PCR). The χ² test, odds ratio (OR), and confidence interval (CI) were calculated using SPSS software to estimate associations and assess the level of risk. Results: Allele frequencies in the general population were 64.4% and 62.8% for HLA DRB1*11 and HLA DRB1*12, respectively. The HLA-DRB1*12 allele was present in 28.9% of cases and 33.9% of controls. The HLA-DRB1*11 allele was present in 32.2% of both cases and controls. In this study, no direct association was found between the presence of the HLA-DRB1*11 and HLA-DRB1*12 alleles and the surveillance of dengue infection. Furthermore, the absence of the HLA-DRB1*11 allele was associated with protection against the development of severe disease (OR = 0.03; 95% CI [0.11 - 0.80]; and p = 0.01). Conclusion: No risk of developing severe dengue fever was found in individuals carrying the HLA-DRB1*11 and HLA-DRB 1*12 alleles. However, further study of other HLA alleles involved in the development of severe dengue may provide more information.