The point mutation analysis of Cyp2C9 2 (Arg144Cys C>T), Cyp2C9 3 (Ile359Leu A>C) and VKORC1 (1639G>A) in women with cervical cancer related to HPV: A case‐control study

• Published in: Journal of cellular physiology Vol. 235; no. 12; pp. 9317 - 9322
• Main Authors: Golshekan, Elham, Sohrabi, Amir
• Format: Journal Article
• Language: English
• Published: United States 01.12.2020
• Subjects: Adult; Alphapapillomavirus - genetics; Cytochrome P-450 CYP2C9 - genetics; Female; Gene Frequency - genetics; Genotype; Humans; Middle Aged; Papillomaviridae - genetics; Papillomaviridae - pathogenicity; Papillomavirus Infections - complications; Papillomavirus Infections - genetics; Point Mutation - genetics; Risk Factors; Uterine Cervical Neoplasms - genetics; Vitamin K Epoxide Reductases - genetics; cytochrome P450; biomarker; cervical cancer; HPV
• ISSN: 0021-9541; 1097-4652
• DOI: 10.1002/jcp.29731

Human papillomavirus (HPV) is the most common sexually transmitted viral infection worldwide. HPV tumorigenesis genotypes are the causative agents of cervical cancer and genital malignancies. The scientific literature has demonstrated that life style, environmental, epigenetic accompanied with HR‐HPV genotypes are potential risk factors for cervical cancer progression. The frequencies of the Cyp2C9* 2 , Cyp2C9* 3 , and vitamin K epoxide reductase complex subunit 1 (VKORC1) genotypes as potential molecular biomarkers have been investigated on Iranian women with cervical malignancy related to HPV genotypes. As a case‐control study, the mutations were appraised using a polymerase chain reaction‐restriction fragment length polymorphism procedure on women suffering from HPV infection (60 cases), CC (46 cases), and 40 subjects of as healthy control. The outcomes demonstrated that Cyp2C9* 3  showed a meaningful relationship between women diagnosed with cervical cancer and the healthy population (AA vs. AC; OR, 7.15; 95% CI, 1.94‐26.3; p = .003). It was also observed that the Cyp2C9* 3 mutation in women with cervical cancer and VKORC1 in healthy population with HPV (+), did not follow the Hardy–Weinberg equilibrium. Our findings aid understanding the genetic polymorphism distribution of Cyp2C9* 2 , Cyp2C9* 3 , and VKORC1 in women with genital malignancies. This can also be useful in predicting the susceptibility risk factors for developing cervical cancer. However, allelic discrimination as a molecular biomarker requires further research.