裸鼠人绿色荧光子宫内膜异位症模型的构建

【目的】 探索建立人子宫内膜异位症荧光体外与在体模型的具体方法65377;【方法】 采用增强型绿色荧光蛋白腺病毒(Ad-eGFP)分别转染原代培养人子宫内膜腺上皮细胞和基质细胞(细胞转染注射法,即方法1)和子宫内膜组织块(组织转染注射法,即方法2),比较两种体外转染的差异;然后采用方法1将绿色荧光腺病毒转染后的腺上皮细胞和基质细胞注入裸鼠皮下形成荧光病灶,并且与方法2相比较,观察直至建模后25 d,计算病灶荧光成模率与病灶荧光存在时间,并给予组织鉴定65377;【结果】 方法1与2建模后5 d两种方法病灶荧光阳性率分别达88.9%和22.2%, P = 0.015;病灶体外荧光存在时间分别为(...

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Published inZhongshan da xue xue bao. Zhongshan daxue xuebao yixue kexue ban = Journal of Sun Yat-sen University. Yi xue ke xue ban Vol. 31
Format Journal Article
LanguageChinese
Published Editorial Office of Journal of Sun Yat-sen University 01.01.2010
Subjects
增强型绿色荧光蛋白腺病毒
子宫内膜异位症
活体无创观察模型
裸鼠
Online AccessGet full text
ISSN1672-3554

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Abstract 【目的】 探索建立人子宫内膜异位症荧光体外与在体模型的具体方法65377;【方法】 采用增强型绿色荧光蛋白腺病毒(Ad-eGFP)分别转染原代培养人子宫内膜腺上皮细胞和基质细胞(细胞转染注射法,即方法1)和子宫内膜组织块(组织转染注射法,即方法2),比较两种体外转染的差异;然后采用方法1将绿色荧光腺病毒转染后的腺上皮细胞和基质细胞注入裸鼠皮下形成荧光病灶,并且与方法2相比较,观察直至建模后25 d,计算病灶荧光成模率与病灶荧光存在时间,并给予组织鉴定65377;【结果】 方法1与2建模后5 d两种方法病灶荧光阳性率分别达88.9%和22.2%, P = 0.015;病灶体外荧光存在时间分别为(12 ± 8) d与(7 ± 4) d65377;病理组织学HE染色和免疫荧光共同鉴定显示病灶来源于人子宫内膜65377; 【结论】 应用Ad-eGFP 转染后人子宫内膜腺上皮细胞和基质细胞混悬液注射可成功建立裸鼠皮下人子宫内膜异位症活体荧光观察模型,同时方法成模率高,体外荧光存在时间较长65377;
AbstractList 【目的】 探索建立人子宫内膜异位症荧光体外与在体模型的具体方法65377;【方法】 采用增强型绿色荧光蛋白腺病毒(Ad-eGFP)分别转染原代培养人子宫内膜腺上皮细胞和基质细胞(细胞转染注射法,即方法1)和子宫内膜组织块(组织转染注射法,即方法2),比较两种体外转染的差异;然后采用方法1将绿色荧光腺病毒转染后的腺上皮细胞和基质细胞注入裸鼠皮下形成荧光病灶,并且与方法2相比较,观察直至建模后25 d,计算病灶荧光成模率与病灶荧光存在时间,并给予组织鉴定65377;【结果】 方法1与2建模后5 d两种方法病灶荧光阳性率分别达88.9%和22.2%, P = 0.015;病灶体外荧光存在时间分别为(12 ± 8) d与(7 ± 4) d65377;病理组织学HE染色和免疫荧光共同鉴定显示病灶来源于人子宫内膜65377; 【结论】 应用Ad-eGFP 转染后人子宫内膜腺上皮细胞和基质细胞混悬液注射可成功建立裸鼠皮下人子宫内膜异位症活体荧光观察模型,同时方法成模率高,体外荧光存在时间较长65377;
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Snippet 【目的】 探索建立人子宫内膜异位症荧光体外与在体模型的具体方法65377;【方法】...
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SubjectTerms 增强型绿色荧光蛋白腺病毒
子宫内膜异位症
活体无创观察模型
裸鼠
Title 裸鼠人绿色荧光子宫内膜异位症模型的构建
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