DJ-1蛋白调控乳鼠心肌细胞内质网应激反应的研究

摘 要: 【目的】 探讨DJ-1蛋白对心肌细胞内质网应激的调控作用。【方法】 在原代培养心肌细胞中采用苯肾上腺素(PE)、毒胡萝卜素(TG)诱导心肌细胞内质网应激。通过腺病毒转染过表达DJ-1蛋白,应用荧光定量PCR及Western blotting分别检测在PE及TG作用下,心肌细胞内质网应激标记物GRP78和calreticulin的mRNA及蛋白表达改变,明确DJ-1对心肌细胞内质网应激的作用。 【结果】 PE、TG均可引起GRP78及calreticulin的mRNA及蛋白表达升高,提示二者均可导致心肌细胞的内质网应激反应。应用腺病毒转染在心肌细胞中过表达DJ-1蛋白,而过表达DJ-1...

Full description

Saved in:
Bibliographic Details
Published inZhongshan da xue xue bao. Zhongshan daxue xuebao yixue kexue ban = Journal of Sun Yat-sen University. Yi xue ke xue ban Vol. 36
Format Journal Article
LanguageChinese
Published Editorial Office of Journal of Sun Yat-sen University 01.01.2015
Subjects
Online AccessGet full text
ISSN1672-3554

Cover

Abstract 摘 要: 【目的】 探讨DJ-1蛋白对心肌细胞内质网应激的调控作用。【方法】 在原代培养心肌细胞中采用苯肾上腺素(PE)、毒胡萝卜素(TG)诱导心肌细胞内质网应激。通过腺病毒转染过表达DJ-1蛋白,应用荧光定量PCR及Western blotting分别检测在PE及TG作用下,心肌细胞内质网应激标记物GRP78和calreticulin的mRNA及蛋白表达改变,明确DJ-1对心肌细胞内质网应激的作用。 【结果】 PE、TG均可引起GRP78及calreticulin的mRNA及蛋白表达升高,提示二者均可导致心肌细胞的内质网应激反应。应用腺病毒转染在心肌细胞中过表达DJ-1蛋白,而过表达DJ-1显著下调内质网应激标记物GRP78及calreticulin的mRNA及蛋白表达,提示DJ-1可抑制PE及TG导致的心肌细胞的内质网应激反应。 【结论】 DJ-1可抑制心肌细胞中PE及TG诱导的内质网应激。
AbstractList 摘 要: 【目的】 探讨DJ-1蛋白对心肌细胞内质网应激的调控作用。【方法】 在原代培养心肌细胞中采用苯肾上腺素(PE)、毒胡萝卜素(TG)诱导心肌细胞内质网应激。通过腺病毒转染过表达DJ-1蛋白,应用荧光定量PCR及Western blotting分别检测在PE及TG作用下,心肌细胞内质网应激标记物GRP78和calreticulin的mRNA及蛋白表达改变,明确DJ-1对心肌细胞内质网应激的作用。 【结果】 PE、TG均可引起GRP78及calreticulin的mRNA及蛋白表达升高,提示二者均可导致心肌细胞的内质网应激反应。应用腺病毒转染在心肌细胞中过表达DJ-1蛋白,而过表达DJ-1显著下调内质网应激标记物GRP78及calreticulin的mRNA及蛋白表达,提示DJ-1可抑制PE及TG导致的心肌细胞的内质网应激反应。 【结论】 DJ-1可抑制心肌细胞中PE及TG诱导的内质网应激。
BookMark eNotjD9Lw0AcQG-oYK39KIG7_C53yShFbaHgonO4XO4kUhtJXNzEaBetUuiignQQ_DMJiqCm9MuYXpzyFRR1eo83vCVU68d9VUN1wrhtgePQRdRM0yjAmDkMbOrVUbvKb6v8scqHVT4qr0_N5bR8yubnd59vz1_5pJhl5dGZ-RiU2U0xOClf7s10VLyP57PD4mL4I-bq2EzG5uF1GS1o0UtV858NtLW2utlqW92N9U5rpWtJYnOwQiID6SgZYgkBY5jaXBImhaKgHSw97SoBMnQoeFoGnlQcNHWBu4wAUO1CA3X-vmEsdvy9JNoVyYEfi8j_DXGy7YtkP5I95dNAaE6UYBoIpQoLjlkAIDyb2wJ7FL4By0lv3Q
ContentType Journal Article
DBID DOA
DatabaseName DOAJ Directory of Open Access Journals
DatabaseTitleList
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
ExternalDocumentID oai_doaj_org_article_4baf71ea6f3144e0a706b33a9272a094
GroupedDBID ALMA_UNASSIGNED_HOLDINGS
GROUPED_DOAJ
ID FETCH-LOGICAL-c1273-d1cbc5ecd0c3b660427c16cae43f50c9f8ea3cd5439fcb9ce73f4837861334f83
IEDL.DBID DOA
ISSN 1672-3554
IngestDate Wed Aug 27 01:31:10 EDT 2025
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Language Chinese
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c1273-d1cbc5ecd0c3b660427c16cae43f50c9f8ea3cd5439fcb9ce73f4837861334f83
OpenAccessLink https://doaj.org/article/4baf71ea6f3144e0a706b33a9272a094
ParticipantIDs doaj_primary_oai_doaj_org_article_4baf71ea6f3144e0a706b33a9272a094
PublicationCentury 2000
PublicationDate 2015-01-01
PublicationDateYYYYMMDD 2015-01-01
PublicationDate_xml – month: 01
  year: 2015
  text: 2015-01-01
  day: 01
PublicationDecade 2010
PublicationTitle Zhongshan da xue xue bao. Zhongshan daxue xuebao yixue kexue ban = Journal of Sun Yat-sen University. Yi xue ke xue ban
PublicationYear 2015
Publisher Editorial Office of Journal of Sun Yat-sen University
Publisher_xml – name: Editorial Office of Journal of Sun Yat-sen University
SSID ssib006563249
ssib001051178
ssib002039699
ssj0002511113
ssib006703418
ssib001103819
ssib002262833
ssib051369397
ssib001186722
ssib043242460
ssib000970529
Score 2.0068355
Snippet 摘 要: 【目的】 探讨DJ-1蛋白对心肌细胞内质网应激的调控作用。【方法】 在原代培养心肌细胞中采用苯肾上腺素(PE)、毒胡萝卜素(TG)诱导心肌细胞内质网应激。通过腺...
SourceID doaj
SourceType Open Website
SubjectTerms 内质网应激
毒胡萝卜素
苯肾上腺素
DJ-1
Title DJ-1蛋白调控乳鼠心肌细胞内质网应激反应的研究
URI https://doaj.org/article/4baf71ea6f3144e0a706b33a9272a094
Volume 36
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrZ2_SxxBFMcHsRCbEI2SxB9Y2C7u7Jud3S0T8TgErTy47piZncHKhGAaK8mpjZogXKOCWAj-qARFiO5x_4x3u1b3Lzizu3BjlSbdMsUsPN6-93k7w_eL0DzokufGwnVCSqRjBJWckPmBoyCKAyaMmXR-QXaVVmtkue7XLasvcyeskAcuArdAOFMBlowq0OwvXRa4lAOwyAs8pmcTU311G7OGqQIcAnOEZQ0-Giws4SpsdMFtITOj62YJ2XkuRNQGdY96oQVGmoLAHlyo_nDIoMobmTuPDAYPHwONoDyvMz3CgD3OvZuxfq9jmv4b04C8u1Xeo3clls59KcIxhoa21sfRyEp58P4BVfvJRT-56SeH_eQoO91Pj9vZbbP3-_L5791Lct7tNLNfB-nTXtY86-7tZvdXafuo-9jqdba7fw71Q3qyk5630uuHCVSrLK0tVp3ShMERWKONE2PBhS9F7ArglBprDoGpYJKA8l0RqVAyELGvwUYJHgkZgMpV6jUnAFEhTKLhjW8b8iOaI1wpCAQx6ySWJMKgN2ci8jgHDOIT-moi0Phe6Gw0jPJ1vqDzoVHmQ-Nf-fD5f2wyhUY1GPnFr5ZpNLz546ec0fCxyWfzPHsF5G7fig
linkProvider Directory of Open Access Journals
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%EF%BC%A4%EF%BC%AA%EF%BC%8D%EF%BC%91%E8%9B%8B%E7%99%BD%E8%B0%83%E6%8E%A7%E4%B9%B3%E9%BC%A0%E5%BF%83%E8%82%8C%E7%BB%86%E8%83%9E%E5%86%85%E8%B4%A8%E7%BD%91%E5%BA%94%E6%BF%80%E5%8F%8D%E5%BA%94%E7%9A%84%E7%A0%94%E7%A9%B6&rft.jtitle=Zhongshan+da+xue+xue+bao.+Zhongshan+daxue+xuebao+yixue+kexue+ban+%3D+Journal+of+Sun+Yat-sen+University.+Yi+xue+ke+xue+ban&rft.date=2015-01-01&rft.pub=Editorial+Office+of+Journal+of+Sun+Yat-sen+University&rft.issn=1672-3554&rft.volume=36&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_4baf71ea6f3144e0a706b33a9272a094
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1672-3554&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1672-3554&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1672-3554&client=summon