FESTA: Flexible Exon-based Splicing and Transcription Annotation

• Published in: bioRxiv
• Main Authors: Rago, Alfredo, Colbourne, John Kenneth
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 17.04.2018; Cold Spring Harbor Laboratory
• Edition: 1.1
• Subjects: Alternative splicing; Bioinformatics; Transcription
• ISSN: 2692-8205; 2692-8205
• DOI: 10.1101/300947

We introduce FESTA, an R based algorithm that allows detection of alternative splicing based on experiment-specific exon expression data. FESTA disentangles alternative splicing signal from whole-gene transcription, facilitating the discovery and characterization of novel regulatory events even in the absence of transcript annotations or paired-end data. We also include customization options to increase its applicability on different platforms and experimental designs as well as a tool for the conversion from transcript expression to inclusion ratios.