Comparison of serum testosterone and estradiol measurements in 3174 European men using platform immunoassay and mass spectrometry; relevance for the diagnostics in aging men
BackgroundThe limitations of serum testosterone and estradiol (E2) measurements using non-extraction platform immunoassays (IAs) are widely recognized. Switching to more specific mass spectrometry (MS)-based methods has been advocated, but directly comparative data on the two methods are scarce.Meth...
Saved in:
| Published in | European journal of endocrinology Vol. 166; no. 6; pp. 983 - 991 |
|---|---|
| Main Authors | , , , , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Bristol
BioScientifica
01.06.2012
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0804-4643 1479-683X 1479-683X |
| DOI | 10.1530/EJE-11-1051 |
Cover
| Abstract | BackgroundThe limitations of serum testosterone and estradiol (E2) measurements using non-extraction platform immunoassays (IAs) are widely recognized. Switching to more specific mass spectrometry (MS)-based methods has been advocated, but directly comparative data on the two methods are scarce.MethodsWe compared serum testosterone and E2 measurements in a large sample of middle-aged/elderly men using a common platform IA and a gas chromatography (GC)–MS method, in order to assess their limitations and advantages, and to diagnose male hypogonadism. Of subjects from the European Male Aging Study (n=3174; age 40–79 years), peripheral serum testosterone and E2 were analyzed using established commercial platform IAs (Roche Diagnostics E170) and in-house GC–MS methods.ResultsOver a broad concentration range, serum testosterone concentration measured by IA and MS showed high correlation (R=0.93, P<0.001), which was less robust in the hypogonadal range (<11 nmol/l; R=0.72, P<0.001). The IA/MS correlation was weaker in E2 measurements (R=0.32, P<0.001, at E2 <40.8 pmol/l, and R=0.74, P<0.001, at E2 >40.8 pmol/l). Using MS as the comparator method, IA ascertained low testosterone compatible with hypogonadism (<11 nmol/l), with 75% sensitivity and 96.3% specificity. The same parameters with IA for the detection of low E2 (<40.7 pmol/l) were 13.3 and 99.3%, and for high E2 (>120 pmol/l) 88.4 and 88.6%.ConclusionA validated platform IA is sufficient to detect subnormal testosterone concentrations in the diagnosis of male hypogonadism. The IA used for E2 measurements showed poor correlation with MS and may only be suitable for the detection of high E2 in men. |
|---|---|
| AbstractList | Background: The limitations of serum testosterone and estradiol (E-2) measurements using nonextraction platform immunoassays (IAs) are widely recognized. Switching to more specific mass spectrometry (MS)-based methods has been advocated, but directly comparative data on the two methods are scarce. Methods: We compared serum testosterone and E-2 measurements in a large sample of middle-aged/elderly men using a common platform IA and a gas chromatography (GC)-MS method, in order to assess their limitations and advantages, and to diagnose male hypogonadism. Of subjects from the European Male Aging Study (n = 3174; age 40-79 years), peripheral serum testosterone and E-2 were analyzed using established commercial platform IAs (Roche Diagnostics E170) and in-house GC MS methods. Results: Over a broad concentration range, serum testosterone concentration measured by IA and MS showed high correlation (R=0.93, P<0.001), which was less robust in the hypogonadal range (<11 nmol/l; R=0.72, P<0.001). The IA/MS correlationwas weaker in E-2 measurements (R=0.32, P<0.001, at E-2 <40.8 pmol/l, and R=0.74, P<0.001, at E-2 >40.8 pmol/l). Using MS as the comparator method, IA ascertained low testosterone compatible with hypogonadism (<11 nmol/l), with 75% sensitivity and 96.3% specificity. The same parameters with IA for the detection of low E-2 (<40.7 pmol/l) were 13.3 and 99.3%, and for high E-2 (>120 pmol/l) 88.4 and 88.6%. Conclusion: A validated platform IA is sufficient to detect subnormal testosterone concentrations in the diagnosis of male hypogonadism. The IA used for E-2 measurements showed poor correlation with MS and may only be suitable for the detection of high E-2 in men. BackgroundThe limitations of serum testosterone and estradiol (E2) measurements using non-extraction platform immunoassays (IAs) are widely recognized. Switching to more specific mass spectrometry (MS)-based methods has been advocated, but directly comparative data on the two methods are scarce.MethodsWe compared serum testosterone and E2 measurements in a large sample of middle-aged/elderly men using a common platform IA and a gas chromatography (GC)–MS method, in order to assess their limitations and advantages, and to diagnose male hypogonadism. Of subjects from the European Male Aging Study (n=3174; age 40–79 years), peripheral serum testosterone and E2 were analyzed using established commercial platform IAs (Roche Diagnostics E170) and in-house GC–MS methods.ResultsOver a broad concentration range, serum testosterone concentration measured by IA and MS showed high correlation (R=0.93, P<0.001), which was less robust in the hypogonadal range (<11 nmol/l; R=0.72, P<0.001). The IA/MS correlation was weaker in E2 measurements (R=0.32, P<0.001, at E2 <40.8 pmol/l, and R=0.74, P<0.001, at E2 >40.8 pmol/l). Using MS as the comparator method, IA ascertained low testosterone compatible with hypogonadism (<11 nmol/l), with 75% sensitivity and 96.3% specificity. The same parameters with IA for the detection of low E2 (<40.7 pmol/l) were 13.3 and 99.3%, and for high E2 (>120 pmol/l) 88.4 and 88.6%.ConclusionA validated platform IA is sufficient to detect subnormal testosterone concentrations in the diagnosis of male hypogonadism. The IA used for E2 measurements showed poor correlation with MS and may only be suitable for the detection of high E2 in men. The limitations of serum testosterone and estradiol (E(2)) measurements using non-extraction platform immunoassays (IAs) are widely recognized. Switching to more specific mass spectrometry (MS)-based methods has been advocated, but directly comparative data on the two methods are scarce.BACKGROUNDThe limitations of serum testosterone and estradiol (E(2)) measurements using non-extraction platform immunoassays (IAs) are widely recognized. Switching to more specific mass spectrometry (MS)-based methods has been advocated, but directly comparative data on the two methods are scarce.We compared serum testosterone and E(2) measurements in a large sample of middle-aged/elderly men using a common platform IA and a gas chromatography (GC)-MS method, in order to assess their limitations and advantages, and to diagnose male hypogonadism. Of subjects from the European Male Aging Study (n=3174; age 40-79 years), peripheral serum testosterone and E(2) were analyzed using established commercial platform IAs (Roche Diagnostics E170) and in-house GC-MS methods.METHODSWe compared serum testosterone and E(2) measurements in a large sample of middle-aged/elderly men using a common platform IA and a gas chromatography (GC)-MS method, in order to assess their limitations and advantages, and to diagnose male hypogonadism. Of subjects from the European Male Aging Study (n=3174; age 40-79 years), peripheral serum testosterone and E(2) were analyzed using established commercial platform IAs (Roche Diagnostics E170) and in-house GC-MS methods.Over a broad concentration range, serum testosterone concentration measured by IA and MS showed high correlation (R=0.93, P<0.001), which was less robust in the hypogonadal range (<11 nmol/l; R=0.72, P<0.001). The IA/MS correlation was weaker in E(2) measurements (R=0.32, P<0.001, at E(2) <40.8 pmol/l, and R=0.74, P<0.001, at E(2) >40.8 pmol/l). Using MS as the comparator method, IA ascertained low testosterone compatible with hypogonadism (<11 nmol/l), with 75% sensitivity and 96.3% specificity. The same parameters with IA for the detection of low E(2) (<40.7 pmol/l) were 13.3 and 99.3%, and for high E(2) (>120 pmol/l) 88.4 and 88.6%.RESULTSOver a broad concentration range, serum testosterone concentration measured by IA and MS showed high correlation (R=0.93, P<0.001), which was less robust in the hypogonadal range (<11 nmol/l; R=0.72, P<0.001). The IA/MS correlation was weaker in E(2) measurements (R=0.32, P<0.001, at E(2) <40.8 pmol/l, and R=0.74, P<0.001, at E(2) >40.8 pmol/l). Using MS as the comparator method, IA ascertained low testosterone compatible with hypogonadism (<11 nmol/l), with 75% sensitivity and 96.3% specificity. The same parameters with IA for the detection of low E(2) (<40.7 pmol/l) were 13.3 and 99.3%, and for high E(2) (>120 pmol/l) 88.4 and 88.6%.A validated platform IA is sufficient to detect subnormal testosterone concentrations in the diagnosis of male hypogonadism. The IA used for E(2) measurements showed poor correlation with MS and may only be suitable for the detection of high E(2) in men.CONCLUSIONA validated platform IA is sufficient to detect subnormal testosterone concentrations in the diagnosis of male hypogonadism. The IA used for E(2) measurements showed poor correlation with MS and may only be suitable for the detection of high E(2) in men. The limitations of serum testosterone and estradiol (E(2)) measurements using non-extraction platform immunoassays (IAs) are widely recognized. Switching to more specific mass spectrometry (MS)-based methods has been advocated, but directly comparative data on the two methods are scarce. We compared serum testosterone and E(2) measurements in a large sample of middle-aged/elderly men using a common platform IA and a gas chromatography (GC)-MS method, in order to assess their limitations and advantages, and to diagnose male hypogonadism. Of subjects from the European Male Aging Study (n=3174; age 40-79 years), peripheral serum testosterone and E(2) were analyzed using established commercial platform IAs (Roche Diagnostics E170) and in-house GC-MS methods. Over a broad concentration range, serum testosterone concentration measured by IA and MS showed high correlation (R=0.93, P<0.001), which was less robust in the hypogonadal range (<11 nmol/l; R=0.72, P<0.001). The IA/MS correlation was weaker in E(2) measurements (R=0.32, P<0.001, at E(2) <40.8 pmol/l, and R=0.74, P<0.001, at E(2) >40.8 pmol/l). Using MS as the comparator method, IA ascertained low testosterone compatible with hypogonadism (<11 nmol/l), with 75% sensitivity and 96.3% specificity. The same parameters with IA for the detection of low E(2) (<40.7 pmol/l) were 13.3 and 99.3%, and for high E(2) (>120 pmol/l) 88.4 and 88.6%. A validated platform IA is sufficient to detect subnormal testosterone concentrations in the diagnosis of male hypogonadism. The IA used for E(2) measurements showed poor correlation with MS and may only be suitable for the detection of high E(2) in men. |
| Author | Kula, Krzysztof Silman, Alan J Wu, Frederick C W Casanueva, Felipe F Huhtaniemi, Ilpo T O'Neill, Terence W Lean, Michael E J Labrie, Fernand Han, Thang S Bartfai, György Giwercman, Aleksander Vanderschueren, Dirk Lee, David M Pendleton, Neil Tajar, Abdelouahid Boonen, Steven Finn, Joseph D Punab, Margus Forti, Gianni |
| Author_xml | – sequence: 1 givenname: Ilpo T surname: Huhtaniemi fullname: Huhtaniemi, Ilpo T – sequence: 2 givenname: Abdelouahid surname: Tajar fullname: Tajar, Abdelouahid – sequence: 3 givenname: David M surname: Lee fullname: Lee, David M – sequence: 4 givenname: Terence W surname: O'Neill fullname: O'Neill, Terence W – sequence: 5 givenname: Joseph D surname: Finn fullname: Finn, Joseph D – sequence: 6 givenname: György surname: Bartfai fullname: Bartfai, György – sequence: 7 givenname: Steven surname: Boonen fullname: Boonen, Steven – sequence: 8 givenname: Felipe F surname: Casanueva fullname: Casanueva, Felipe F – sequence: 9 givenname: Aleksander surname: Giwercman fullname: Giwercman, Aleksander – sequence: 10 givenname: Thang S surname: Han fullname: Han, Thang S – sequence: 11 givenname: Krzysztof surname: Kula fullname: Kula, Krzysztof – sequence: 12 givenname: Fernand surname: Labrie fullname: Labrie, Fernand – sequence: 13 givenname: Michael E J surname: Lean fullname: Lean, Michael E J – sequence: 14 givenname: Neil surname: Pendleton fullname: Pendleton, Neil – sequence: 15 givenname: Margus surname: Punab fullname: Punab, Margus – sequence: 16 givenname: Alan J surname: Silman fullname: Silman, Alan J – sequence: 17 givenname: Dirk surname: Vanderschueren fullname: Vanderschueren, Dirk – sequence: 18 givenname: Gianni surname: Forti fullname: Forti, Gianni – sequence: 19 givenname: Frederick C W surname: Wu fullname: Wu, Frederick C W |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25986807$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/22423144$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9kk2LFDEQhhtZcWdXT94lF0GQ1nz2dONJhvGLBS8K3kJ1uno20knaJK3Mj_I_mvlwBQ97CKmQp-qtVN6r6sIHj1X1lNFXTAn6evtpWzNWM6rYg2rF5Lqrm1Z8u6hWtKWylo0Ul9VVSt8pZSWmj6pLziUXTMpV9XsT3AzRpuBJGEnCuDiSMeWQMsYiRMAPpJwjDDZMxCGkJaJDnxOxngi2lmS7xDAj-HLryZKs35F5gjyG6Ih1bvEBUoL9sZQrIUkzmhyDwxz3b0jECX-CN0hKBsm3SAYLO186sOYoArtDyVL8cfVwhCnhk_N-XX19t_2y-VDffH7_cfP2pu6VkrkeKcVxoN26a7jAFpCpURmuGiXaQY6c9lIgQtMJTse-kQMg7_sODUcclOrEdQWnuukXzkuv52gdxL0OYPUcYoZJR0wI0dzqadEJdaEmayDb4JPum4EDV6BFg42WrDe65Yxr1isxroEpaUTReHHSmGP4sZQJa2eTwWkCj2FJmlG-pqItq6DPzujSOxzu2vn7jQV4fgYgGZjGWKZp0z9OdW3T0nXhXp44E0NKEcc7hFF9MJMuZtKM6YOZCs3-o43NxycWN9jp_pzehmRssYkdy1zu1fkD4FHg6Q |
| CitedBy_id | crossref_primary_10_1016_j_beem_2014_09_008 crossref_primary_10_22141_2224_0721_17_1_2021_226434 crossref_primary_10_1016_j_clinbiochem_2020_03_008 crossref_primary_10_1111_cen_15157 crossref_primary_10_3390_nano10071346 crossref_primary_10_3389_fendo_2019_00858 crossref_primary_10_1007_s00774_013_0466_5 crossref_primary_10_1111_j_1464_410X_2012_11465_x crossref_primary_10_1016_j_microc_2021_106395 crossref_primary_10_2174_1871530322666220516160435 crossref_primary_10_1038_s41366_024_01518_2 crossref_primary_10_1007_s40618_018_0897_x crossref_primary_10_62968_2070_9781_2024_25_4_47_54 crossref_primary_10_1007_s11695_017_2802_7 crossref_primary_10_1111_cen_13530 crossref_primary_10_1111_andr_12850 crossref_primary_10_1111_j_1365_2605_2012_01266_x crossref_primary_10_1016_j_acuro_2017_01_007 crossref_primary_10_1016_j_jsxm_2017_10_067 crossref_primary_10_1186_1472_6890_14_33 crossref_primary_10_1002_jbmr_3088 crossref_primary_10_1111_andr_12506 crossref_primary_10_1210_en_2014_1890 crossref_primary_10_1016_j_eururo_2021_06_007 crossref_primary_10_1016_j_steroids_2015_08_013 crossref_primary_10_1007_s40618_022_01776_9 crossref_primary_10_1530_EJE_16_0447 crossref_primary_10_1007_s11154_022_09746_5 crossref_primary_10_1530_EJE_13_0222 crossref_primary_10_3390_jcm11040956 crossref_primary_10_1007_s11154_013_9243_y crossref_primary_10_3390_ijerph182212168 crossref_primary_10_1111_jsm_12327 crossref_primary_10_3389_fendo_2023_1320722 crossref_primary_10_1530_JOE_13_0393 crossref_primary_10_1186_s12902_024_01663_9 crossref_primary_10_5534_wjmh_200017 crossref_primary_10_1016_j_jsxm_2021_01_187 crossref_primary_10_1007_s11154_023_09870_w crossref_primary_10_3390_jcm12165354 crossref_primary_10_1111_cen_13797 crossref_primary_10_1016_j_endoen_2014_11_008 crossref_primary_10_1080_21505594_2016_1259053 crossref_primary_10_1016_j_jchromb_2021_123002 crossref_primary_10_1016_j_mayocp_2016_02_014 crossref_primary_10_1373_clinchem_2013_213363 crossref_primary_10_1016_j_cca_2014_07_036 crossref_primary_10_1016_j_fertnstert_2013_02_039 crossref_primary_10_1177_0004563213501478 crossref_primary_10_1007_s12020_015_0650_6 crossref_primary_10_1093_humrep_dez255 crossref_primary_10_1371_journal_pone_0109346 crossref_primary_10_1007_s10552_013_0336_4 crossref_primary_10_1371_journal_pone_0100409 crossref_primary_10_1093_humrep_dex078 crossref_primary_10_1515_cclm_2017_1201 crossref_primary_10_1016_j_maturitas_2019_04_203 crossref_primary_10_1210_clinem_dgab164 crossref_primary_10_1111_cen_13305 crossref_primary_10_3389_fendo_2020_00414 crossref_primary_10_3390_biom10121697 crossref_primary_10_1210_clinem_dgad586 crossref_primary_10_1530_EJE_15_0757 crossref_primary_10_1038_s41393_024_01056_8 crossref_primary_10_1016_j_psyneuen_2025_107379 crossref_primary_10_1210_er_2014_1024 crossref_primary_10_1007_s10304_017_0124_4 crossref_primary_10_1007_s40618_020_01304_7 crossref_primary_10_1002_jcla_24739 crossref_primary_10_1210_er_2018_00178 crossref_primary_10_1111_bju_13516 crossref_primary_10_1038_nrendo_2012_52 crossref_primary_10_12688_f1000research_16561_1 crossref_primary_10_1210_jc_2014_4184 crossref_primary_10_1371_journal_pone_0201912 crossref_primary_10_1111_jsm_12546 crossref_primary_10_1007_s12020_018_1543_2 crossref_primary_10_4155_bio_2015_0025 crossref_primary_10_1515_cclm_2024_1237 crossref_primary_10_1210_jc_2013_3375 crossref_primary_10_1111_jsm_12146 crossref_primary_10_1530_EJE_15_0380 crossref_primary_10_1016_j_acuroe_2017_12_003 crossref_primary_10_1210_endocr_bqad108 crossref_primary_10_7554_eLife_74638 crossref_primary_10_1007_s40618_014_0055_z crossref_primary_10_1007_s40619_014_0091_0 crossref_primary_10_1111_andr_12770 crossref_primary_10_1111_andr_13460 crossref_primary_10_1016_j_maturitas_2015_11_003 crossref_primary_10_1007_s11427_023_2305_0 crossref_primary_10_1007_s40618_020_01243_3 crossref_primary_10_1007_s00216_021_03586_z crossref_primary_10_1210_endocr_bqac001 crossref_primary_10_1186_s12915_023_01755_3 crossref_primary_10_3390_biom11040553 crossref_primary_10_1200_JCO_2014_59_1602 crossref_primary_10_1016_j_jsxm_2020_01_018 crossref_primary_10_1016_j_beem_2022_101622 crossref_primary_10_1007_s40618_021_01598_1 crossref_primary_10_1111_cen_13723 crossref_primary_10_1016_j_fertnstert_2013_04_009 crossref_primary_10_1016_j_fertnstert_2014_07_1248 crossref_primary_10_1007_s40618_023_02218_w crossref_primary_10_1210_jc_2012_3861 crossref_primary_10_1111_and_12779 crossref_primary_10_4274_jus_galenos_2023_2023_0013 crossref_primary_10_1007_s11154_022_09763_4 crossref_primary_10_1111_j_2047_2927_2013_00141_x crossref_primary_10_1093_toxsci_kfu021 crossref_primary_10_3389_fnbeh_2022_984494 crossref_primary_10_3238_PersUro_2015_0911_04 crossref_primary_10_1152_physrev_00033_2015 crossref_primary_10_1111_jsm_12404 crossref_primary_10_1210_jendso_bvae107 crossref_primary_10_1111_j_2047_2927_2014_00206_x crossref_primary_10_1097_MED_0000000000000068 crossref_primary_10_1111_andr_12636 crossref_primary_10_1016_j_metabol_2018_03_007 crossref_primary_10_1007_s13566_019_00375_1 crossref_primary_10_1039_C5AY02796A crossref_primary_10_23736_S1825_859X_20_00077_8 crossref_primary_10_1007_s11154_022_09752_7 crossref_primary_10_30841_2307_5090_4_2022_274432 crossref_primary_10_1016_j_clinbiochem_2018_05_008 crossref_primary_10_1016_S0140_6736_13_61126_5 crossref_primary_10_1016_j_bone_2020_115818 crossref_primary_10_1016_j_cca_2012_10_007 crossref_primary_10_1111_jsm_13044 crossref_primary_10_4103_aja2024104 crossref_primary_10_1111_ijcp_12235 crossref_primary_10_1177_1403494814545103 crossref_primary_10_18632_oncotarget_19816 crossref_primary_10_1016_j_beem_2013_05_002 crossref_primary_10_1016_j_evolhumbehav_2019_08_005 crossref_primary_10_5534_wjmh_221027 crossref_primary_10_1007_s00216_023_04963_6 crossref_primary_10_1016_j_endonu_2014_09_002 crossref_primary_10_1038_ijo_2013_228 |
| Cites_doi | 10.1158/1055-9965.EPI-06-0792 10.1210/jc.2007-0530 10.1210/jc.2010-3012 10.1210/jc.2009-2354 10.1359/jbmr.080518 10.1016/S0140-6736(86)90837-8 10.1373/49.8.1381 10.1210/jc.2010-2369 10.1016/j.jsbmb.2006.02.004 10.1016/j.cca.2007.07.013 10.1677/erc.0.0060315 10.1016/j.amjcard.2008.03.029 10.1111/j.1365-2265.2011.04017.x 10.1210/jc.2003-031287 10.1097/gme.0b013e3181d76768 10.1016/j.jsbmb.2006.09.034 10.1214/aos/1176346394 10.1016/j.steroids.2010.01.012 10.1210/jc.2010-1314 10.4158/EP.4.1.1 10.1359/jbmr.0810c 10.1056/NEJMoa0911101 10.1016/j.atherosclerosis.2011.01.053 10.1111/j.1365-2605.2008.00879.x 10.1210/jc.84.10.3666 10.1007/s00198-008-0591-5 10.1210/jc.2005-0962 10.1016/j.jsbmb.2010.03.032 10.1210/jc.2010-1123 |
| ContentType | Journal Article |
| Contributor | Kula, K Lage, Mary Corona, Giovanni Casanueva, F F Vanderschueren, D Jiang, Min Slowikowska-Hilczer, Jolanta Pye, Stephen O'Neill, T W Lee, D M Fejes, Imre Tajar, A Steer, Philip Finn, J D Boonen, S Punab, M Silman, A J Castro, Ana I Petrone, Luisa Borghs, Herman Wu, F C W Bartfai, G Huhtaniemi, I T Forti, G Walczak-Jedrzejowska, Renata Giwercman, A Földesi, Imre Korrovitz, Paul |
| Contributor_xml | – sequence: 1 givenname: G surname: Forti fullname: Forti, G – sequence: 2 givenname: Luisa surname: Petrone fullname: Petrone, Luisa – sequence: 3 givenname: Giovanni surname: Corona fullname: Corona, Giovanni – sequence: 4 givenname: D surname: Vanderschueren fullname: Vanderschueren, D – sequence: 5 givenname: S surname: Boonen fullname: Boonen, S – sequence: 6 givenname: Herman surname: Borghs fullname: Borghs, Herman – sequence: 7 givenname: K surname: Kula fullname: Kula, K – sequence: 8 givenname: Jolanta surname: Slowikowska-Hilczer fullname: Slowikowska-Hilczer, Jolanta – sequence: 9 givenname: Renata surname: Walczak-Jedrzejowska fullname: Walczak-Jedrzejowska, Renata – sequence: 10 givenname: I T surname: Huhtaniemi fullname: Huhtaniemi, I T – sequence: 11 givenname: A surname: Giwercman fullname: Giwercman, A – sequence: 12 givenname: F C W surname: Wu fullname: Wu, F C W – sequence: 13 givenname: A J surname: Silman fullname: Silman, A J – sequence: 14 givenname: T W surname: O'Neill fullname: O'Neill, T W – sequence: 15 givenname: J D surname: Finn fullname: Finn, J D – sequence: 16 givenname: Philip surname: Steer fullname: Steer, Philip – sequence: 17 givenname: A surname: Tajar fullname: Tajar, A – sequence: 18 givenname: D M surname: Lee fullname: Lee, D M – sequence: 19 givenname: Stephen surname: Pye fullname: Pye, Stephen – sequence: 20 givenname: F F surname: Casanueva fullname: Casanueva, F F – sequence: 21 givenname: Mary surname: Lage fullname: Lage, Mary – sequence: 22 givenname: Ana I surname: Castro fullname: Castro, Ana I – sequence: 23 givenname: G surname: Bartfai fullname: Bartfai, G – sequence: 24 givenname: Imre surname: Földesi fullname: Földesi, Imre – sequence: 25 givenname: Imre surname: Fejes fullname: Fejes, Imre – sequence: 26 givenname: M surname: Punab fullname: Punab, M – sequence: 27 givenname: Paul surname: Korrovitz fullname: Korrovitz, Paul – sequence: 28 givenname: Min surname: Jiang fullname: Jiang, Min |
| Copyright | 2012 European Society of Endocrinology 2015 INIST-CNRS |
| Copyright_xml | – notice: 2012 European Society of Endocrinology – notice: 2015 INIST-CNRS |
| CorporateAuthor | EMAS Group Reproductive medicine, Malmö Faculty of Medicine Institutionen för translationell medicin Reproduktionsmedicin, Malmö Department of Translational Medicine Lunds universitet Medicinska fakulteten Lund University |
| CorporateAuthor_xml | – name: EMAS Group – name: Faculty of Medicine – name: Medicinska fakulteten – name: Lund University – name: Reproductive medicine, Malmö – name: Department of Translational Medicine – name: Reproduktionsmedicin, Malmö – name: Lunds universitet – name: Institutionen för translationell medicin |
| DBID | AAYXX CITATION IQODW CGR CUY CVF ECM EIF NPM 7X8 ADTPV AOWAS D95 |
| DOI | 10.1530/EJE-11-1051 |
| DatabaseName | CrossRef Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic SwePub SwePub Articles SWEPUB Lunds universitet |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine Anatomy & Physiology |
| DocumentTitleAlternate | Testosterone and estradiol assays |
| EISSN | 1479-683X |
| EndPage | 991 |
| ExternalDocumentID | oai_portal_research_lu_se_publications_b6d2a25a_36e6_41bc_8212_1b53f7a154c3 22423144 25986807 10_1530_EJE_11_1051 |
| Genre | Multicenter Study Comparative Study Research Support, Non-U.S. Gov't Journal Article |
| GeographicLocations | Europe |
| GeographicLocations_xml | – name: Europe |
| GroupedDBID | --- -~X .55 .GJ 08R 0R~ 18M 2WC 3O- 4.4 53G 5GY 5RE 5VS 5WD AABZA AACZT AAPBV AAPGJ AAPXW AAUGY AAVAP AAWDT ABLYK ABOCM ABPQP ABPTD ABPTK ABSGY ABSQV ABWST ABXZS ACFRR ACGFO ACNCT ACPRK ACUTJ ADBBV ADDZX ADIPN ADVEK AENEX AFFNX AFGWE AFHIN AFYAG AGQXC AGUTN AI. AJEEA ALMA_UNASSIGNED_HOLDINGS ALXQX ANFBD APJGH BAWUL BCRHZ BTFSW C1A CS3 DIK DU5 E3Z EBS EJD EMOBN F9R GX1 H13 HZ~ IL9 INIJC J5H KQ8 L7B O9- OAUYM OBFPC OBOKY OCZFY OK1 OPAEJ OVD OWPYF P2P RHF ROX SJN TBS TCN TEORI TMA TR2 VH1 W8F WOQ X7M ZA5 ZGI ZXP AARHZ AAUAY AAYXX ABDFA ABEJV ABGNP ABIME ABJNI ABMNT ABNHQ ABPIB ABQNK ABVGC ABXVV ADGKP ADMTO ADNBA ADQBN ADVOB AEMQT AFXAL AGORE AHGBF AHMMS AJBYB AJNCP ATGXG CITATION KOP OJZSN IQODW NU- CGR CUY CVF ECM EIF NPM 7X8 ADTPV AOWAS D95 |
| ID | FETCH-LOGICAL-b554t-f00efd0979623e8ae15f5c256538d4f20b43eea69320fb64dae2bb9ec2eed5593 |
| ISSN | 0804-4643 1479-683X |
| IngestDate | Tue Sep 09 22:27:50 EDT 2025 Sun Sep 28 00:01:35 EDT 2025 Mon Jul 21 06:01:04 EDT 2025 Mon Jul 21 09:16:03 EDT 2025 Tue Jul 01 04:13:22 EDT 2025 Thu Apr 24 23:00:32 EDT 2025 Tue Oct 31 02:10:29 EDT 2023 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 6 |
| Keywords | Human Senescence Androgen Ageing Estrogen Exploration Male European Estradiol Ovarian hormone Immunological method Relevance Testosterone Serum Testicular hormone Diagnosis Sex steroid hormone Mass spectrometry Endocrinology Comparative study Public health |
| Language | English |
| License | CC BY 4.0 |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-b554t-f00efd0979623e8ae15f5c256538d4f20b43eea69320fb64dae2bb9ec2eed5593 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
| OpenAccessLink | http://dx.doi.org/10.1530/EJE-11-1051 |
| PMID | 22423144 |
| PQID | 1027038703 |
| PQPubID | 23479 |
| PageCount | 9 |
| ParticipantIDs | swepub_primary_oai_portal_research_lu_se_publications_b6d2a25a_36e6_41bc_8212_1b53f7a154c3 proquest_miscellaneous_1027038703 pubmed_primary_22423144 pascalfrancis_primary_25986807 crossref_primary_10_1530_EJE_11_1051 crossref_citationtrail_10_1530_EJE_11_1051 bioscientifica_primary_10_1530_EJE_11_1051 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2012-06-01 |
| PublicationDateYYYYMMDD | 2012-06-01 |
| PublicationDate_xml | – month: 06 year: 2012 text: 2012-06-01 day: 01 |
| PublicationDecade | 2010 |
| PublicationPlace | Bristol |
| PublicationPlace_xml | – name: Bristol – name: England |
| PublicationTitle | European journal of endocrinology |
| PublicationTitleAlternate | Eur J Endocrinol |
| PublicationYear | 2012 |
| Publisher | BioScientifica |
| Publisher_xml | – name: BioScientifica |
| References | Taieb (5_17737102) 2003; 49 (12_38086177) 2010; 95 (30_42719885) 1998; 4 Bhasin (8_37377972) 2010; 95 Bland (24_13727057) 1986; 1 Khosla (27_31854719) 2008; 19 Labrie (19_22203854) 2006; 99 Ahmed (29_31435760) 2008; 102 Moal (2_29494539) 2007; 386 Wang (1_18030918) 2004; 89 (11_38745903) 2009; 74 (10_36465610) 2008; 73 Hsing (31_28554389) 2007; 16 Callou de Sa (16_39811410) 2011; 75 Rosner (3_38206183) 2010; 95 Gennari (15_31992314) 2008; 23 (25_42719884) 1984; 12 Wartofsky (9_38745824) 2010; 95 Whyte (14_30250082) 2008; 23 Lee (18_33419026) 2009; 32 (22_40142769) 2011; 96 Blair (7_36623048) 2010; 75 Labrie (20_23579894) 2007; 103 Vesper (4_36915277) 2010; 121 (21_28903671) 2007; 92 (26_37460055) 2010; 363 Jeon (17_37381431) 2010; 17 Vermeulen (23_10996884) 1999; 84 (6_19578262) 2005; 90 Braunstein (13_6521006) 1999; 6 Chen (28_39380285) 2011; 216 |
| References_xml | – volume: 73 start-page: 1311 issn: 1878-5867 year: 2008 ident: 10_36465610 – volume: 16 start-page: 1004 issn: 1055-9965 issue: 5 year: 2007 ident: 31_28554389 publication-title: Cancer Epidemiology Biomarkers & Prevention doi: 10.1158/1055-9965.EPI-06-0792 – volume: 92 start-page: 3697 issn: 0021-972X issue: 9 year: 2007 ident: 21_28903671 publication-title: Journal of Clinical Endocrinology & Metabolism doi: 10.1210/jc.2007-0530 – volume: 96 start-page: 2430 issn: 0021-972X issue: 8 year: 2011 ident: 22_40142769 publication-title: Journal of Clinical Endocrinology & Metabolism doi: 10.1210/jc.2010-3012 – volume: 95 start-page: 2536 issn: 0021-972X issue: 6 year: 2010 ident: 8_37377972 publication-title: Journal of Clinical Endocrinology & Metabolism doi: 10.1210/jc.2009-2354 – volume: 23 start-page: 1552 issn: 0884-0431 issue: 10 year: 2008 ident: 14_30250082 publication-title: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research doi: 10.1359/jbmr.080518 – volume: 1 start-page: 307 issn: 0140-6736 issue: 8476 year: 1986 ident: 24_13727057 publication-title: Lancet doi: 10.1016/S0140-6736(86)90837-8 – volume: 49 start-page: 1381 issn: 0009-9147 issue: 8 year: 2003 ident: 5_17737102 publication-title: Clinical Chemistry doi: 10.1373/49.8.1381 – volume: 95 start-page: 5141 issn: 0021-972X issue: 12 year: 2010 ident: 9_38745824 publication-title: Journal of Clinical Endocrinology & Metabolism doi: 10.1210/jc.2010-2369 – volume: 99 start-page: 182 issn: 0960-0760 issue: 4-5 year: 2006 ident: 19_22203854 publication-title: The Journal of steroid biochemistry and molecular biology doi: 10.1016/j.jsbmb.2006.02.004 – volume: 386 start-page: 12 issn: 1873-3492 issue: 1-2 year: 2007 ident: 2_29494539 publication-title: Clinica Chimica Acta doi: 10.1016/j.cca.2007.07.013 – volume: 6 start-page: 315 issn: 1351-0088 issue: 2 year: 1999 ident: 13_6521006 publication-title: Endocrine-Related Cancer doi: 10.1677/erc.0.0060315 – volume: 102 start-page: 150 issn: 0002-9149 issue: 2 year: 2008 ident: 29_31435760 publication-title: The American journal of cardiology doi: 10.1016/j.amjcard.2008.03.029 – volume: 75 start-page: 177 issn: 0300-0664 issue: 2 year: 2011 ident: 16_39811410 publication-title: Clinical endocrinology doi: 10.1111/j.1365-2265.2011.04017.x – volume: 89 start-page: 534 issn: 0021-972X issue: 2 year: 2004 ident: 1_18030918 publication-title: Journal of Clinical Endocrinology & Metabolism doi: 10.1210/jc.2003-031287 – volume: 17 start-page: 902 issn: 1072-3714 issue: 5 year: 2010 ident: 17_37381431 publication-title: Menopause (New York, N.Y.) doi: 10.1097/gme.0b013e3181d76768 – volume: 103 start-page: 178 issn: 0960-0760 issue: 2 year: 2007 ident: 20_23579894 publication-title: The Journal of steroid biochemistry and molecular biology doi: 10.1016/j.jsbmb.2006.09.034 – volume: 12 start-page: 87 year: 1984 ident: 25_42719884 publication-title: ANNALS OF STATISTICS doi: 10.1214/aos/1176346394 – volume: 75 start-page: 297 issn: 1878-5867 issue: 4-5 year: 2010 ident: 7_36623048 doi: 10.1016/j.steroids.2010.01.012 – volume: 95 start-page: 4542 issn: 0021-972X issue: 10 year: 2010 ident: 3_38206183 publication-title: Journal of Clinical Endocrinology & Metabolism doi: 10.1210/jc.2010-1314 – volume: 4 start-page: 1 year: 1998 ident: 30_42719885 publication-title: ENDOCRINE PRACTICE doi: 10.4158/EP.4.1.1 – volume: 23 start-page: 1548 issn: 0884-0431 issue: 10 year: 2008 ident: 15_31992314 publication-title: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research doi: 10.1359/jbmr.0810c – volume: 363 start-page: 123 issn: 0028-4793 issue: 2 year: 2010 ident: 26_37460055 publication-title: New England Journal of Medicine doi: 10.1056/NEJMoa0911101 – volume: 216 start-page: 414 issn: 1879-1484 issue: 2 year: 2011 ident: 28_39380285 doi: 10.1016/j.atherosclerosis.2011.01.053 – volume: 74 start-page: 498 issn: 1878-5867 year: 2009 ident: 11_38745903 – volume: 32 start-page: 11 issn: 0105-6263 issue: 1 year: 2009 ident: 18_33419026 publication-title: International journal of andrology doi: 10.1111/j.1365-2605.2008.00879.x – volume: 84 start-page: 3666 issn: 0021-972X issue: 10 year: 1999 ident: 23_10996884 publication-title: Journal of Clinical Endocrinology & Metabolism doi: 10.1210/jc.84.10.3666 – volume: 19 start-page: 1465 issn: 0937-941X issue: 10 year: 2008 ident: 27_31854719 publication-title: Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA doi: 10.1007/s00198-008-0591-5 – volume: 90 start-page: 5928 issn: 0021-972X issue: 11 year: 2005 ident: 6_19578262 publication-title: Journal of Clinical Endocrinology & Metabolism doi: 10.1210/jc.2005-0962 – volume: 121 start-page: 513 issn: 0960-0760 issue: 3-5 year: 2010 ident: 4_36915277 publication-title: The Journal of steroid biochemistry and molecular biology doi: 10.1016/j.jsbmb.2010.03.032 – volume: 95 start-page: 5305 issn: 0021-972X issue: 12 year: 2010 ident: 12_38086177 publication-title: Journal of Clinical Endocrinology & Metabolism doi: 10.1210/jc.2010-1123 |
| SSID | ssj0016430 |
| Score | 2.4538586 |
| Snippet | BackgroundThe limitations of serum testosterone and estradiol (E2) measurements using non-extraction platform immunoassays (IAs) are widely recognized.... The limitations of serum testosterone and estradiol (E(2)) measurements using non-extraction platform immunoassays (IAs) are widely recognized. Switching to... Background: The limitations of serum testosterone and estradiol (E-2) measurements using nonextraction platform immunoassays (IAs) are widely recognized.... |
| SourceID | swepub proquest pubmed pascalfrancis crossref bioscientifica |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 983 |
| SubjectTerms | Adult Aged Aging - blood Biological and medical sciences Clinical Medicine Clinical Study Cohort Studies Electrochemistry Endocrinology and Diabetes Endocrinopathies Endokrinologi och diabetes Estradiol - blood Europe Fundamental and applied biological sciences. Psychology Gas Chromatography-Mass Spectrometry Humans Hypogonadism - blood Hypogonadism - diagnosis Immunoassay - methods Klinisk medicin Luminescent Measurements Male Medical and Health Sciences Medical sciences Medicin och hälsovetenskap Middle Aged Miscellaneous Public health. Hygiene Public health. Hygiene-occupational medicine Sensitivity and Specificity Serum Albumin - metabolism Sex Hormone-Binding Globulin - metabolism Testosterone - blood Vertebrates: endocrinology |
| Title | Comparison of serum testosterone and estradiol measurements in 3174 European men using platform immunoassay and mass spectrometry; relevance for the diagnostics in aging men |
| URI | http://dx.doi.org/10.1530/EJE-11-1051 https://www.ncbi.nlm.nih.gov/pubmed/22423144 https://www.proquest.com/docview/1027038703 |
| Volume | 166 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1tb9MwELaqISEQINh4KS-TkSY-UGVLE-dNfCrT0Jg0JKROTHyx7NjRitqk2pIP4z_xN_hd3Nlp4kKFGF-iKLGdpPfUvjs_d0fIXqFSAYZP6qkwzT2WjSNPMpl7UcJUiun3YkPGPP0UH5-xk_PofDD46bCWmlru5983xpX8j1ThGsgVo2RvINluULgA5yBfOIKE4fhPMj50iwiO4JHNYlRjqRjMflCVdmdAozNDzSqMEun8gYYFC4s6G3XueLg8aoznYDkXNeqyoxkGj1SgXgubpWkBpyMTm4lJDurLaywAgFVXDI-gYywqS98zCaDhMbYO0qKNOdu0C9BqxLpUFUxi657-4-aixjD4haEdfJwvq57XPRXfLD98IpWeV424WPHzO4aR4ez3Ll_k9qDf2zi-p22K3S-u6wM5JLHr-ng_q8wEaEhVwp04feax2KZ_2td2YmdJ5sWpqT3cz_y24EsLcXcez9LQUQkyW1Dsj9UmCpGeeXRy5BleIEby3woS0OGQHPC5382CVzG-vtV7tXGi0P3A6XyX3Jczm8HUftGaqnRvKa7gX1vYciub7KHfkt0aBWn6kDxoLRs6sTB9RAa63CY7k1LU1eKavqGGa2xEu01un7aUjh3yowcxrQpqQExdEFNAHu1ATF0Q01lJEcR0hSa4W1IDYroCMXVAbIZCEFMXxO9oB2EKPShAmDoQxocYCOPgj8nZh6Pp4bHX1hDxJCjKtVf4vi6UnyUZ6Pk6FXocFVEOej4s9IoVgS9ZqLWIwYzxCxkzJXQgZabzAJRHsLbDJ2SrhG99RqgO8ixUYZgGRcp8AZq7FEGqRaFYEqhUDsnbdfnxpU0Yw9HQBmFzEDZY3ByFDY1XsuV5m40fi8LMNzfe6xr_dczdNZB0bQMswpD6yZC8XqGGwyqCW4Oi1FVzBd2DBIksfjgkTy2c-t5oco0ZG5KvFl_dHUxNb70EvE1NdsHnDb_SfOnsOXAZq0AEkeBhrGPOxjLnKWjMfCyjsEgEGHF5-PwmP94LcqefDl6Srfqy0a_ANqjlrvnj_QIZTBnS |
| linkProvider | Colorado Alliance of Research Libraries |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Comparison+of+serum+testosterone+and+estradiol+measurements+in+3174+European+men+using+platform+immunoassay+and+mass+spectrometry%3B+relevance+for+the+diagnostics+in+aging+men&rft.jtitle=European+journal+of+endocrinology&rft.au=Huhtaniemi%2C+Ilpo+T&rft.au=Tajar%2C+Abdelouahid&rft.au=Lee%2C+David+M&rft.au=O%27Neill%2C+Terence+W&rft.date=2012-06-01&rft.pub=BioScientifica&rft.issn=0804-4643&rft.eissn=1479-683X&rft.volume=166&rft.issue=6&rft.spage=983&rft.epage=991&rft_id=info:doi/10.1530%2FEJE-11-1051 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0804-4643&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0804-4643&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0804-4643&client=summon |