The Apoptotic Effect of Green Tea (−)-Epigallocatechin Gallate on 3T3-L1 Preadipocytes Depends on the Cdk2 Pathway
This study was designed to investigate the effect of green tea catechins, especially (−)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1 preadipocytes. Preadipocyte apoptosis as indicated by formation of DNA fragments was induced by EGCG in dose-dependent manners. While EGCG was demonstr...
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| Published in | Journal of agricultural and food chemistry Vol. 53; no. 14; pp. 5695 - 5701 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Washington, DC
American Chemical Society
13.07.2005
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0021-8561 1520-5118 |
| DOI | 10.1021/jf050045p |
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| Abstract | This study was designed to investigate the effect of green tea catechins, especially (−)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1 preadipocytes. Preadipocyte apoptosis as indicated by formation of DNA fragments was induced by EGCG in dose-dependent manners. While EGCG was demonstrated to decrease Cdk2 expression and activity and increase caspase-3 activity, overexpression of Cdk2 and treatment with the caspase-3 inhibitor respectively prevented preadipocytes from induction of DNA fragmentation and caspase-3 activity by doses of 100−400 μM of EGCG. This suggests the Cdk2- and caspase-3-dependent apoptotic effects of EGCG. Moreover, EGCG was more effective than EC, ECG, and EGC in changing the apoptotic signals. Results of this study may relate to the mechanism by which EGCG modulates body weight. Keywords: Green tea; epigallocatechin gallate; 3T3-L1 preadipocyte; cyclin-dependent kinase; caspase-3; apoptosis |
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| AbstractList | This study was designed to investigate the effect of green tea catechins, especially (−)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1 preadipocytes. Preadipocyte apoptosis as indicated by formation of DNA fragments was induced by EGCG in dose-dependent manners. While EGCG was demonstrated to decrease Cdk2 expression and activity and increase caspase-3 activity, overexpression of Cdk2 and treatment with the caspase-3 inhibitor respectively prevented preadipocytes from induction of DNA fragmentation and caspase-3 activity by doses of 100−400 μM of EGCG. This suggests the Cdk2- and caspase-3-dependent apoptotic effects of EGCG. Moreover, EGCG was more effective than EC, ECG, and EGC in changing the apoptotic signals. Results of this study may relate to the mechanism by which EGCG modulates body weight. Keywords: Green tea; epigallocatechin gallate; 3T3-L1 preadipocyte; cyclin-dependent kinase; caspase-3; apoptosis This study was designed to investigate the effect of green tea catechins, especially (-)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1 preadipocytes. Preadipocyte apoptosis as indicated by formation of DNA fragments was induced by EGCG in dose-dependent manners. While EGCG was demonstrated to decrease Cdk2 expression and activity and increase caspase-3 activity, overexpression of Cdk2 and treatment with the caspase-3 inhibitor respectively prevented preadipocytes from induction of DNA fragmentation and caspase-3 activity by doses of 100-400 muM of EGCG. This suggests the Cdk2- and caspase-3-dependent apoptotic effects of EGCG. Moreover, EGCG was more effective than EC, ECG, and EGC in changing the apoptotic signals. Results of this study may relate to the mechanism by which EGCG modulates body weight. This study was designed to investigate the effect of green tea catechins, especially (-)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1 preadipocytes. Preadipocyte apoptosis as indicated by formation of DNA fragments was induced by EGCG in dose-dependent manners. While EGCG was demonstrated to decrease Cdk2 expression and activity and increase caspase-3 activity, overexpression of Cdk2 and treatment with the caspase-3 inhibitor respectively prevented preadipocytes from induction of DNA fragmentation and caspase-3 activity by doses of 100-400 micromolar of EGCG. This suggests the Cdk2- and caspase-3-dependent apoptotic effects of EGCG. Moreover, EGCG was more effective than EC, ECG, and EGC in changing the apoptotic signals. Results of this study may relate to the mechanism by which EGCG modulates body weight. This study was designed to investigate the effect of green tea catechins, especially (-)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1 preadipocytes. Preadipocyte apoptosis as indicated by formation of DNA fragments was induced by EGCG in dose-dependent manners. While EGCG was demonstrated to decrease Cdk2 expression and activity and increase caspase-3 activity, overexpression of Cdk2 and treatment with the caspase-3 inhibitor respectively prevented preadipocytes from induction of DNA fragmentation and caspase-3 activity by doses of 100-400 muM of EGCG. This suggests the Cdk2- and caspase-3-dependent apoptotic effects of EGCG. Moreover, EGCG was more effective than EC, ECG, and EGC in changing the apoptotic signals. Results of this study may relate to the mechanism by which EGCG modulates body weight.This study was designed to investigate the effect of green tea catechins, especially (-)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1 preadipocytes. Preadipocyte apoptosis as indicated by formation of DNA fragments was induced by EGCG in dose-dependent manners. While EGCG was demonstrated to decrease Cdk2 expression and activity and increase caspase-3 activity, overexpression of Cdk2 and treatment with the caspase-3 inhibitor respectively prevented preadipocytes from induction of DNA fragmentation and caspase-3 activity by doses of 100-400 muM of EGCG. This suggests the Cdk2- and caspase-3-dependent apoptotic effects of EGCG. Moreover, EGCG was more effective than EC, ECG, and EGC in changing the apoptotic signals. Results of this study may relate to the mechanism by which EGCG modulates body weight. |
| Author | Wu, Bo-Tsung Chen, Hui-Chian Kao, Yung-Hsi Hung, Pei-Fang Huang, Rong-Nan Chang, Hsin-Huei |
| Author_xml | – sequence: 1 givenname: Bo-Tsung surname: Wu fullname: Wu, Bo-Tsung – sequence: 2 givenname: Pei-Fang surname: Hung fullname: Hung, Pei-Fang – sequence: 3 givenname: Hui-Chian surname: Chen fullname: Chen, Hui-Chian – sequence: 4 givenname: Rong-Nan surname: Huang fullname: Huang, Rong-Nan – sequence: 5 givenname: Hsin-Huei surname: Chang fullname: Chang, Hsin-Huei – sequence: 6 givenname: Yung-Hsi surname: Kao fullname: Kao, Yung-Hsi |
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| Keywords | Flavanol 3T3-L1 preadipocyte epigallocatechin gallate Enzyme Transferases Caspase apoptosis Flavonoid Peptidases Polyphenol Kinase caspase- 3 Hydrolases cyclin-dependent kinase Green tea |
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| Snippet | This study was designed to investigate the effect of green tea catechins, especially (−)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1... This study was designed to investigate the effect of green tea catechins, especially (-)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1... |
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| SubjectTerms | 3T3 Cells adipocytes Adipocytes - cytology Adipocytes - drug effects Animals apoptosis Apoptosis - drug effects Biological and medical sciences Camellia sinensis - chemistry Caspase 3 Caspases - metabolism Catechin - analogs & derivatives Catechin - pharmacology CDC2-CDC28 Kinases - genetics CDC2-CDC28 Kinases - metabolism Coffee, tea and other stimulative beverage industries cyclin-dependent kinase Cyclin-Dependent Kinase 2 cysteine proteinases DNA Fragmentation enzyme activity epigallocatechin gallate fibroblasts flavanols Food industries Fundamental and applied biological sciences. Psychology Gene Expression green tea Mice preadipocytes Stem Cells - cytology Stem Cells - drug effects Transfection |
| Title | The Apoptotic Effect of Green Tea (−)-Epigallocatechin Gallate on 3T3-L1 Preadipocytes Depends on the Cdk2 Pathway |
| URI | http://dx.doi.org/10.1021/jf050045p https://api.istex.fr/ark:/67375/TPS-T237SVLM-R/fulltext.pdf https://www.ncbi.nlm.nih.gov/pubmed/15998135 https://www.proquest.com/docview/46702575 https://www.proquest.com/docview/68003336 |
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