A Rapidly Modulated Multifocal Detection Scheme for Parallel Acquisition of Raman Spectra from a 2‑D Focal Array
We report the development of a rapidly modulated multifocal detection scheme that enables full Raman spectra (∼500–2000 cm–1) from a 2-D focal array to be acquired simultaneously. A spatial light modulator splits a laser beam to generate an m × n multifocal array. Raman signals generated within each...
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          | Published in | Analytical chemistry (Washington) Vol. 86; no. 13; pp. 6604 - 6609 | 
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| Main Authors | , | 
| Format | Journal Article | 
| Language | English | 
| Published | 
        United States
          American Chemical Society
    
        01.07.2014
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| Subjects | |
| Online Access | Get full text | 
| ISSN | 0003-2700 1520-6882 1520-6882  | 
| DOI | 10.1021/ac5012188 | 
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| Abstract | We report the development of a rapidly modulated multifocal detection scheme that enables full Raman spectra (∼500–2000 cm–1) from a 2-D focal array to be acquired simultaneously. A spatial light modulator splits a laser beam to generate an m × n multifocal array. Raman signals generated within each focus are projected simultaneously into a spectrometer and imaged onto a TE-cooled CCD camera. A shuttering system using different masks is constructed to collect the superimposed Raman spectra of different multifocal patterns. The individual Raman spectrum from each focus is then retrieved from the superimposed spectra with no crosstalk using a postacquisition data processing algorithm. This system is expected to significantly improve the speed of current Raman-based instruments such as laser tweezers Raman spectroscopy and hyperspectral Raman imaging. | 
    
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| AbstractList | We report the development of a rapidly modulated multifocal detection scheme that enables full Raman spectra (∼500–2000 cm–1) from a 2-D focal array to be acquired simultaneously. A spatial light modulator splits a laser beam to generate an m × n multifocal array. Raman signals generated within each focus are projected simultaneously into a spectrometer and imaged onto a TE-cooled CCD camera. A shuttering system using different masks is constructed to collect the superimposed Raman spectra of different multifocal patterns. The individual Raman spectrum from each focus is then retrieved from the superimposed spectra with no crosstalk using a postacquisition data processing algorithm. This system is expected to significantly improve the speed of current Raman-based instruments such as laser tweezers Raman spectroscopy and hyperspectral Raman imaging. We report the development of a rapidly modulated multifocal detection scheme that enables full Raman spectra (~500-2000 cm(-1)) from a 2-D focal array to be acquired simultaneously. A spatial light modulator splits a laser beam to generate an m × n multifocal array. Raman signals generated within each focus are projected simultaneously into a spectrometer and imaged onto a TE-cooled CCD camera. A shuttering system using different masks is constructed to collect the superimposed Raman spectra of different multifocal patterns. The individual Raman spectrum from each focus is then retrieved from the superimposed spectra with no crosstalk using a postacquisition data processing algorithm. This system is expected to significantly improve the speed of current Raman-based instruments such as laser tweezers Raman spectroscopy and hyperspectral Raman imaging. We report the development of a rapidly modulated multifocal detection scheme that enables full Raman spectra (~500-2000 cm(-1)) from a 2-D focal array to be acquired simultaneously. A spatial light modulator splits a laser beam to generate an m × n multifocal array. Raman signals generated within each focus are projected simultaneously into a spectrometer and imaged onto a TE-cooled CCD camera. A shuttering system using different masks is constructed to collect the superimposed Raman spectra of different multifocal patterns. The individual Raman spectrum from each focus is then retrieved from the superimposed spectra with no crosstalk using a postacquisition data processing algorithm. This system is expected to significantly improve the speed of current Raman-based instruments such as laser tweezers Raman spectroscopy and hyperspectral Raman imaging.We report the development of a rapidly modulated multifocal detection scheme that enables full Raman spectra (~500-2000 cm(-1)) from a 2-D focal array to be acquired simultaneously. A spatial light modulator splits a laser beam to generate an m × n multifocal array. Raman signals generated within each focus are projected simultaneously into a spectrometer and imaged onto a TE-cooled CCD camera. A shuttering system using different masks is constructed to collect the superimposed Raman spectra of different multifocal patterns. The individual Raman spectrum from each focus is then retrieved from the superimposed spectra with no crosstalk using a postacquisition data processing algorithm. This system is expected to significantly improve the speed of current Raman-based instruments such as laser tweezers Raman spectroscopy and hyperspectral Raman imaging. We report the development of a rapidly modulated multifocal detection scheme that enables full Raman spectra (500-2000 cm...) from a 2-D focal array to be acquired simultaneously. A spatial light modulator splits a laser beam to generate an m x n multifocal array. Raman signals generated within each focus are projected simultaneously into a spectrometer and imaged onto a TE-cooled CCD camera. A shuttering system using different masks is constructed to collect the superimposed Raman spectra of different multifocal patterns. The individual Raman spectrum from each focus is then retrieved from the superimposed spectra with no crosstalk using a postacquisition data processing algorithm. This system is expected to significantly improve the speed of current Raman-based instruments such as laser tweezers Raman spectroscopy and hyperspectral Raman imaging. (ProQuest: ... denotes formulae/symbols omitted.) We report the development of a rapidly modulated multifocal detection scheme that enables full Raman spectra (∼500–2000 cm–¹) from a 2-D focal array to be acquired simultaneously. A spatial light modulator splits a laser beam to generate an m × n multifocal array. Raman signals generated within each focus are projected simultaneously into a spectrometer and imaged onto a TE-cooled CCD camera. A shuttering system using different masks is constructed to collect the superimposed Raman spectra of different multifocal patterns. The individual Raman spectrum from each focus is then retrieved from the superimposed spectra with no crosstalk using a postacquisition data processing algorithm. This system is expected to significantly improve the speed of current Raman-based instruments such as laser tweezers Raman spectroscopy and hyperspectral Raman imaging.  | 
    
| Author | Kong, Lingbo Chan, James  | 
    
| AuthorAffiliation | Department of Pathology and Laboratory Medicine Center for Biophotonics University of California, Davis  | 
    
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| References | Xie C. G. (ref1/cit1) 2002; 27 Chan J. W. (ref4/cit4) 2013; 6 Dufresne E. R. (ref11/cit11) 2001; 72 Di Leonardo R. (ref16/cit16) 2006; 15 Singh G. P. (ref7/cit7) 2005; 77 Chan J. W. (ref5/cit5) 2008; 80 Zhang P. F. (ref10/cit10) 2010; 35 Qi J. (ref14/cit14) 2013; 4 Kong L. B. (ref8/cit8) 2010; 82 Liu R. (ref9/cit9) 2010; 64 Petrov D. V. (ref2/cit2) 2007; 9 Polin M. (ref15/cit15) 2005; 13 Kong L. B. (ref3/cit3) 2011; 6 Qi J. (ref13/cit13) 2012; 37 Chen D. (ref6/cit6) 2006; 78 Sow C. H. (ref12/cit12) 2004; 78  | 
    
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| SubjectTerms | Algorithms Analytical chemistry Arrays cameras Data processing Equipment Design image analysis Imaging Raman spectra Raman spectroscopy Shuttering Spectrometers Spectrum analysis Spectrum Analysis, Raman - instrumentation Spectrum Analysis, Raman - methods Symbols Time Factors  | 
    
| Title | A Rapidly Modulated Multifocal Detection Scheme for Parallel Acquisition of Raman Spectra from a 2‑D Focal Array | 
    
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