Column Characterization and Selection Systems in Reversed-Phase High-Performance Liquid Chromatography

Reversed-phase high-performance liquid chromatography (RP-HPLC) is the most popular chromatographic mode, accounting for more than 90% of all separations. HPLC itself owes its immense popularity to it being relatively simple and inexpensive, with the equipment being reliable and easy to operate. Due...

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Published inChemical reviews Vol. 119; no. 6; pp. 3674 - 3729
Main Authors Žuvela, Petar, Skoczylas, Magdalena, Jay Liu, J, Ba̧czek, Tomasz, Kaliszan, Roman, Wong, Ming Wah, Buszewski, Bogusław
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 27.03.2019
Subjects
Online AccessGet full text
ISSN0009-2665
1520-6890
1520-6890
DOI10.1021/acs.chemrev.8b00246

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Abstract Reversed-phase high-performance liquid chromatography (RP-HPLC) is the most popular chromatographic mode, accounting for more than 90% of all separations. HPLC itself owes its immense popularity to it being relatively simple and inexpensive, with the equipment being reliable and easy to operate. Due to extensive automation, it can be run virtually unattended with multiple samples at various separation conditions, even by relatively low-skilled personnel. Currently, there are >600 RP-HPLC columns available to end users for purchase, some of which exhibit very large differences in selectivity and production quality. Often, two similar RP-HPLC columns are not equally suitable for the requisite separation, and to date, there is no universal RP-HPLC column covering a variety of analytes. This forces analytical laboratories to keep a multitude of diverse columns. Therefore, column selection is a crucial segment of RP-HPLC method development, especially since sample complexity is constantly increasing. Rationally choosing an appropriate column is complicated. In addition to the differences in the primary intermolecular interactions with analytes of the dispersive (London) type, individual columns can also exhibit a unique character owing to specific polar, hydrogen bond, and electron pair donor–acceptor interactions. They can also vary depending on the type of packing, amount and type of residual silanols, “end-capping”, bonding density of ligands, and pore size, among others. Consequently, the chromatographic performance of RP-HPLC systems is often considerably altered depending on the selected column. Although a wide spectrum of knowledge is available on this important subject, there is still a lack of a comprehensive review for an objective comparison and/or selection of chromatographic columns. We aim for this review to be a comprehensive, authoritative, critical, and easily readable monograph of the most relevant publications regarding column selection and characterization in RP-HPLC covering the past four decades. Future perspectives, which involve the integration of state-of-the-art molecular simulations (molecular dynamics or Monte Carlo) with minimal experiments, aimed at nearly “experiment-free” column selection methodology, are proposed.
AbstractList Reversed-phase high-performance liquid chromatography (RP-HPLC) is the most popular chromatographic mode, accounting for more than 90% of all separations. HPLC itself owes its immense popularity to it being relatively simple and inexpensive, with the equipment being reliable and easy to operate. Due to extensive automation, it can be run virtually unattended with multiple samples at various separation conditions, even by relatively low-skilled personnel. Currently, there are >600 RP-HPLC columns available to end users for purchase, some of which exhibit very large differences in selectivity and production quality. Often, two similar RP-HPLC columns are not equally suitable for the requisite separation, and to date, there is no universal RP-HPLC column covering a variety of analytes. This forces analytical laboratories to keep a multitude of diverse columns. Therefore, column selection is a crucial segment of RP-HPLC method development, especially since sample complexity is constantly increasing. Rationally choosing an appropriate column is complicated. In addition to the differences in the primary intermolecular interactions with analytes of the dispersive (London) type, individual columns can also exhibit a unique character owing to specific polar, hydrogen bond, and electron pair donor–acceptor interactions. They can also vary depending on the type of packing, amount and type of residual silanols, “end-capping”, bonding density of ligands, and pore size, among others. Consequently, the chromatographic performance of RP-HPLC systems is often considerably altered depending on the selected column. Although a wide spectrum of knowledge is available on this important subject, there is still a lack of a comprehensive review for an objective comparison and/or selection of chromatographic columns. We aim for this review to be a comprehensive, authoritative, critical, and easily readable monograph of the most relevant publications regarding column selection and characterization in RP-HPLC covering the past four decades. Future perspectives, which involve the integration of state-of-the-art molecular simulations (molecular dynamics or Monte Carlo) with minimal experiments, aimed at nearly “experiment-free” column selection methodology, are proposed.
Reversed-phase high-performance liquid chromatography (RP-HPLC) is the most popular chromatographic mode, accounting for more than 90% of all separations. HPLC itself owes its immense popularity to it being relatively simple and inexpensive, with the equipment being reliable and easy to operate. Due to extensive automation, it can be run virtually unattended with multiple samples at various separation conditions, even by relatively low-skilled personnel. Currently, there are >600 RP-HPLC columns available to end users for purchase, some of which exhibit very large differences in selectivity and production quality. Often, two similar RP-HPLC columns are not equally suitable for the requisite separation, and to date, there is no universal RP-HPLC column covering a variety of analytes. This forces analytical laboratories to keep a multitude of diverse columns. Therefore, column selection is a crucial segment of RP-HPLC method development, especially since sample complexity is constantly increasing. Rationally choosing an appropriate column is complicated. In addition to the differences in the primary intermolecular interactions with analytes of the dispersive (London) type, individual columns can also exhibit a unique character owing to specific polar, hydrogen bond, and electron pair donor-acceptor interactions. They can also vary depending on the type of packing, amount and type of residual silanols, "end-capping", bonding density of ligands, and pore size, among others. Consequently, the chromatographic performance of RP-HPLC systems is often considerably altered depending on the selected column. Although a wide spectrum of knowledge is available on this important subject, there is still a lack of a comprehensive review for an objective comparison and/or selection of chromatographic columns. We aim for this review to be a comprehensive, authoritative, critical, and easily readable monograph of the most relevant publications regarding column selection and characterization in RP-HPLC covering the past four decades. Future perspectives, which involve the integration of state-of-the-art molecular simulations (molecular dynamics or Monte Carlo) with minimal experiments, aimed at nearly "experiment-free" column selection methodology, are proposed.Reversed-phase high-performance liquid chromatography (RP-HPLC) is the most popular chromatographic mode, accounting for more than 90% of all separations. HPLC itself owes its immense popularity to it being relatively simple and inexpensive, with the equipment being reliable and easy to operate. Due to extensive automation, it can be run virtually unattended with multiple samples at various separation conditions, even by relatively low-skilled personnel. Currently, there are >600 RP-HPLC columns available to end users for purchase, some of which exhibit very large differences in selectivity and production quality. Often, two similar RP-HPLC columns are not equally suitable for the requisite separation, and to date, there is no universal RP-HPLC column covering a variety of analytes. This forces analytical laboratories to keep a multitude of diverse columns. Therefore, column selection is a crucial segment of RP-HPLC method development, especially since sample complexity is constantly increasing. Rationally choosing an appropriate column is complicated. In addition to the differences in the primary intermolecular interactions with analytes of the dispersive (London) type, individual columns can also exhibit a unique character owing to specific polar, hydrogen bond, and electron pair donor-acceptor interactions. They can also vary depending on the type of packing, amount and type of residual silanols, "end-capping", bonding density of ligands, and pore size, among others. Consequently, the chromatographic performance of RP-HPLC systems is often considerably altered depending on the selected column. Although a wide spectrum of knowledge is available on this important subject, there is still a lack of a comprehensive review for an objective comparison and/or selection of chromatographic columns. We aim for this review to be a comprehensive, authoritative, critical, and easily readable monograph of the most relevant publications regarding column selection and characterization in RP-HPLC covering the past four decades. Future perspectives, which involve the integration of state-of-the-art molecular simulations (molecular dynamics or Monte Carlo) with minimal experiments, aimed at nearly "experiment-free" column selection methodology, are proposed.
Author Jay Liu, J
Buszewski, Bogusław
Ba̧czek, Tomasz
Wong, Ming Wah
Skoczylas, Magdalena
Kaliszan, Roman
Žuvela, Petar
AuthorAffiliation Department of Chemistry
Nicolaus Copernicus University
Department of Pharmaceutical Chemistry
Department of Chemical Engineering
Department of Environmental Chemistry and Bioanalytics, Center for Modern Interdisciplinary Technologies
Department of Biopharmaceutics and Pharmacodynamics
AuthorAffiliation_xml – name: Nicolaus Copernicus University
– name: Department of Biopharmaceutics and Pharmacodynamics
– name: Department of Chemical Engineering
– name: Department of Chemistry
– name: Department of Pharmaceutical Chemistry
– name: Department of Environmental Chemistry and Bioanalytics, Center for Modern Interdisciplinary Technologies
Author_xml – sequence: 1
  givenname: Petar
  orcidid: 0000-0001-6481-2241
  surname: Žuvela
  fullname: Žuvela, Petar
  organization: Department of Chemistry
– sequence: 2
  givenname: Magdalena
  surname: Skoczylas
  fullname: Skoczylas, Magdalena
  organization: Nicolaus Copernicus University
– sequence: 3
  givenname: J
  orcidid: 0000-0003-4274-2355
  surname: Jay Liu
  fullname: Jay Liu, J
  email: jayliu@pknu.ac.kr
  organization: Department of Chemical Engineering
– sequence: 4
  givenname: Tomasz
  orcidid: 0000-0001-6468-1974
  surname: Ba̧czek
  fullname: Ba̧czek, Tomasz
– sequence: 5
  givenname: Roman
  surname: Kaliszan
  fullname: Kaliszan, Roman
– sequence: 6
  givenname: Ming Wah
  orcidid: 0000-0003-2162-1220
  surname: Wong
  fullname: Wong, Ming Wah
  organization: Department of Chemistry
– sequence: 7
  givenname: Bogusław
  surname: Buszewski
  fullname: Buszewski, Bogusław
  organization: Nicolaus Copernicus University
BackLink https://www.ncbi.nlm.nih.gov/pubmed/30604951$$D View this record in MEDLINE/PubMed
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Snippet Reversed-phase high-performance liquid chromatography (RP-HPLC) is the most popular chromatographic mode, accounting for more than 90% of all separations. HPLC...
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SubjectTerms automation
Chromatography
Computer simulation
End users
High-performance liquid chromatography
human resources
hydrogen bonding
Hydrogen bonds
ligands
Liquid chromatography
Molecular dynamics
Pore size
Porosity
reversed-phase high performance liquid chromatography
Selectivity
Separation
Title Column Characterization and Selection Systems in Reversed-Phase High-Performance Liquid Chromatography
URI http://dx.doi.org/10.1021/acs.chemrev.8b00246
https://www.ncbi.nlm.nih.gov/pubmed/30604951
https://www.proquest.com/docview/2202201150
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https://www.proquest.com/docview/2221025519
Volume 119
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