Structure-Based Design of Novel Class II c-Met Inhibitors: 2. SAR and Kinase Selectivity Profiles of the Pyrazolone Series

• Published in: Journal of medicinal chemistry Vol. 55; no. 5; pp. 1868 - 1897
• Main Authors: Liu, Longbin, Norman, Mark H, Lee, Matthew, Xi, Ning, Siegmund, Aaron, Boezio, Alessandro A, Booker, Shon, Choquette, Debbie, D’Angelo, Noel D, Germain, Julie, Yang, Kevin, Yang, Yajing, Zhang, Yihong, Bellon, Steven F, Whittington, Douglas A, Harmange, Jean-Christophe, Dominguez, Celia, Kim, Tae-Seong, Dussault, Isabelle
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 08.03.2012; Amer Chemical Soc
• Subjects: Aminopyridines - chemical synthesis; Aminopyridines - chemistry; Aminopyridines - pharmacology; Animals; Antineoplastic Agents - chemical synthesis; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Cell Line, Tumor; Chemistry, Medicinal; Crystallography, X-Ray; Drug Design; Gastrins - metabolism; Humans; Life Sciences & Biomedicine; Male; Mice; Models, Molecular; Pharmacology & Pharmacy; Phosphorylation; Protein Conformation; Proto-Oncogene Proteins c-met - antagonists & inhibitors; Proto-Oncogene Proteins c-met - metabolism; Pyrazoles - chemical synthesis; Pyrazoles - chemistry; Pyrazoles - pharmacology; Pyrazolones - chemical synthesis; Pyrazolones - chemistry; Pyrazolones - pharmacology; Rats; Receptor, IGF Type 1 - antagonists & inhibitors; Science & Technology; Stereoisomerism; Structure-Activity Relationship; Vascular Endothelial Growth Factor Receptor-2 - antagonists & inhibitors; TARGET; GROWTH-FACTOR RECEPTOR; POTENT; METASTASIS; SIGNALING PATHWAY; TYROSINE KINASE; IDENTIFICATION; SCATTER-FACTOR; CANCER; DISCOVERY
• ISSN: 0022-2623; 1520-4804; 1520-4804
• DOI: 10.1021/jm201331s

As part of our effort toward developing an effective therapeutic agent for c-Met-dependent tumors, a pyrazolone-based class II c-Met inhibitor, N-(4-((6,7-dimethoxyquinolin-4-yl)­oxy)-3-fluorophenyl)-1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazole-4-carboxamide (1), was identified. Knowledge of the binding mode of this molecule in both c-Met and VEGFR-2 proteins led to a novel strategy for designing more selective analogues of 1. Along with detailed SAR information, we demonstrate that the low kinase selectivity associated with class II c-Met inhibitors can be improved significantly. This work resulted in the discovery of potent c-Met inhibitors with improved selectivity profiles over VEGFR-2 and IGF-1R that could serve as useful tools to probe the relationship between kinase selectivity and in vivo efficacy in tumor xenograft models. Compound 59e (AMG 458) was ultimately advanced into preclinical safety studies.