An S‑Shaped Aβ42 Cross‑β Hexamer Embedded into a Lipid Bilayer Reveals Membrane Disruption and Permeability

• Published in: ACS chemical neuroscience Vol. 14; no. 5; pp. 936 - 946
• Main Authors: Nguyen, Phuong H, Derreumaux, Philippe
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.03.2023; American Chemical Society (ACS)
• Subjects: Amyloid beta-Peptides - metabolism; Chemical Sciences; Lipid Bilayers - metabolism; Molecular Dynamics Simulation; Peptide Fragments - metabolism; Permeability; water permeability; amyloid; membrane; Alzheimer’s disease; oligomers; simulations
• ISSN: 1948-7193; 1948-7193
• DOI: 10.1021/acschemneuro.2c00785

The interactions of amyloid oligomers with membranes are known to contribute to cellular toxicity. Numerous in vitro experimental studies reported on the insertion of oligomers of different sizes that can induce cell membrane disruption, extract lipids, and form ion-permeable transmembrane pores. The current repertoire of amyloid-beta (Aβ) membrane-inserted folds that was subject to high-resolution structure NMR spectroscopy and computer simulations is devoid of any cross-β fibrillar structure. In this study, we explored the dynamics of an S-shaped Aβ42 cross-β hexamer model inserted into a lipid bilayer membrane by two atomistic molecular dynamics simulations. The initial model is characterized by the hydrophobic residues at the central hydrophobic core (residues 17–21, CHC) and the C-terminus (residues 30–42) embedded into the membrane. We observed major structural secondary, tertiary, and quaternary rearrangements leading to two distinct species, hexamer and two trimers, accompanied by membrane disruption and water permeation. The simulations show that some configurations, but not the majority, have the CHC and C-terminus hydrophobic residues exposed to the solvent. Overall, our computational results offer new perspectives to understand the relationship between Aβ42 assemblies and membrane permeability.