Fluorescence Quenching in Microheterogeneous Media: A Laboratory Experiment Determining Micelle Aggregation Number

In the present work we describe a simple procedure for measuring micelle aggregation numbers of surfactants, which has been designed to introduce students to heterogeneous systems and the techniques by which their physical and chemical properties may be determined. This method is based on the spectr...

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Bibliographic Details
Published inJournal of chemical education Vol. 72; no. 7; p. 662
Main Authors Prieto, M. Flor Rodríguez, Rodríguez, M. Carmen Ríos, González, Manuel Mosquera, Rodríguez, Ana M. Ríos, Fernández, Juan Carlos Mejuto
Format Journal Article
LanguageEnglish
Published Division of Chemical Education 01.07.1995
Subjects
Second-Year Undergraduate
Hands-On Learning / Manipulatives
Biochemistry
Micelles
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ISSN0021-9584
1938-1328
DOI10.1021/ed072p662

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Summary:In the present work we describe a simple procedure for measuring micelle aggregation numbers of surfactants, which has been designed to introduce students to heterogeneous systems and the techniques by which their physical and chemical properties may be determined. This method is based on the spectroscopic properties of molecules acting as "probes" and it provides, the student with additional experience in fluorescence spectroscopy. By using this technique, with sodium dodecyl sulfate as surfactant, cetylpiridinium chloride as quencher, and pyrene as the probe, micelle aggregation numbers in good agreement with published values can be found.
ISSN:0021-9584
1938-1328
DOI:10.1021/ed072p662