Protein Hydrolysate Batch Production with Angiotensin I-Converting Enzyme Inhibitory Activity from Egg Whites
運用thermolysin以製備卵蛋白胜肽，經定性並分區以探討各區對ACE活性之抑制，並應用自發性高血壓大鼠(SHR)測試卵蛋白胜肽的抗高血壓功效。在thermolysin最適條件(pH 8及60℃)，及[E]/[S]＝0.02%下，1%粗卵蛋白溶液經水解4小時，離心後澄清液中之產物回收率約80%，此水解物有高度ACE抑制活性(IC(下標 50)＝33 μg/mL)，不具苦味亦無蛋清之腥味，依序經分子量10,000，3,000和1,000 Da膜之超過濾，可提高其濾液對ACE之抑制活性達IC(下標 50)=17 μg/mL。Thermolysin可回收重複使用四次而不降低水解物之ACE活性抑制...
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| Published in | 藥物食品分析 Vol. 14; no. 4; pp. 385 - 390 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
衛生福利部食品藥物管理署
01.12.2006
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1021-9498 |
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| Abstract | 運用thermolysin以製備卵蛋白胜肽，經定性並分區以探討各區對ACE活性之抑制，並應用自發性高血壓大鼠(SHR)測試卵蛋白胜肽的抗高血壓功效。在thermolysin最適條件(pH 8及60℃)，及[E]/[S]＝0.02%下，1%粗卵蛋白溶液經水解4小時，離心後澄清液中之產物回收率約80%，此水解物有高度ACE抑制活性(IC(下標 50)＝33 μg/mL)，不具苦味亦無蛋清之腥味，依序經分子量10,000，3,000和1,000 Da膜之超過濾，可提高其濾液對ACE之抑制活性達IC(下標 50)=17 μg/mL。Thermolysin可回收重複使用四次而不降低水解物之ACE活性抑制，SHR實驗顯示口服0.2 g/Kg體重能有效降低高血壓。 |
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| AbstractList | 運用thermolysin以製備卵蛋白胜肽，經定性並分區以探討各區對ACE活性之抑制，並應用自發性高血壓大鼠(SHR)測試卵蛋白胜肽的抗高血壓功效。在thermolysin最適條件(pH 8及60℃)，及[E]/[S]＝0.02%下，1%粗卵蛋白溶液經水解4小時，離心後澄清液中之產物回收率約80%，此水解物有高度ACE抑制活性(IC(下標 50)＝33 μg/mL)，不具苦味亦無蛋清之腥味，依序經分子量10,000，3,000和1,000 Da膜之超過濾，可提高其濾液對ACE之抑制活性達IC(下標 50)=17 μg/mL。Thermolysin可回收重複使用四次而不降低水解物之ACE活性抑制，SHR實驗顯示口服0.2 g/Kg體重能有效降低高血壓。 |
| Author | 蔡正宗(Tsun-Chung Tsai) 郭偉翔(Wei-Shiang Guo) 江文德(Wen-Dee Chiang) 李明展(Ming-Jan Lee) |
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| Keywords | angiotensin I-converting enzyme ACE 蛋白 血管收縮I-轉化脢 蛋白質水解物 protein hydrolysate 抗高血壓活性 egg whites antihypertensive activity |
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| Title | Protein Hydrolysate Batch Production with Angiotensin I-Converting Enzyme Inhibitory Activity from Egg Whites |
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