Biochemistry laboratory manual for undergraduates : an inquiry-based approach

Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classi...

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Bibliographic Details
Main Authors: Gerczei, Timea, (Author), Pattison, Scott, 1947- (Author)
Other Authors: Rulka, Anna, (Editor)
Format: eBook
Language: English
Published: Warsaw [Poland] ; Berlin [Germany] : De Gruyter Open, 2014.
Subjects:
Biochemistry > Laboratory manuals.
Molecular biology > Laboratory manuals.
Drug resistance in microorganisms > Laboratory manuals.
elektronické knihy
electronic books
ISBN: 9783110411331
3110411334
9781523100705
1523100702
3110411326
9783110411324
Physical Description: 1 online resource (186 pages) : illustrations (some color)

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Table of contents

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008 150531t20142014pl a o 000 0 eng d
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020 |a 9783110411331  |q (electronic bk.) 
020 |a 3110411334  |q (electronic bk.) 
020 |a 9781523100705  |q (electronic bk.) 
020 |a 1523100702  |q (electronic bk.) 
020 |a 3110411326 
020 |a 9783110411324 
020 |z 9783110411324 
035 |a (OCoLC)912309482  |z (OCoLC)939262791  |z (OCoLC)945782837 
100 1 |a Gerczei, Timea,  |e author. 
245 1 0 |a Biochemistry laboratory manual for undergraduates :  |b an inquiry-based approach /  |c Timea Gerczei, Scott Pattison ; managing editor, Anna Rulka. 
264 1 |a Warsaw [Poland] ;  |a Berlin [Germany] :  |b De Gruyter Open,  |c 2014. 
264 4 |c ©2014 
300 |a 1 online resource (186 pages) :  |b illustrations (some color) 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
506 |a Plný text je dostupný pouze z IP adres počítačů Univerzity Tomáše Bati ve Zlíně nebo vzdáleným přístupem pro zaměstnance a studenty 
520 |a Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic. 
505 0 |a Machine generated contents note: 1. Introducing the Bacterial Antibiotic Sensor Mini Project -- 1.1. What are Antibiotics? -- 1.2. What is Bacterial Antibiotic Resistance? -- 1.3. How Do the Bacteria Detect Antibiotics In Its Environment? -- 1.4. How Does the ykkCD Sensor Exert Its Function? -- 1.5. What Do We Do During the Mini Project? -- 2. Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- 2.1. Learning Objectives -- 2.2. Mini Project Flowchart -- 2.3. Why is Sequence Conservation Important for Macromolecule Function, and How Do We Determine This? -- 2.4. Review of Nucleic Acid Properties -- 2.5. What is Bioinformatics? -- 2.6. Identifying Conserved Sequence Elements (Invariable Blocks) -- 2.7. Identifying Conserved Structural Elements -- BLAST Prelab -- Identifying Invariable Blocks in the Toxin Sensor Lab Report Outline and Point Distribution -- BLAst Problem Set -- Protein Properties Worksheet. 
505 0 |a Note continued: 3. Designing Primers for Site-Directed Mutagenesis -- 3.1. Learning Objectives -- 3.2. Mini Project Flowchart -- 3.3. What is PCR? What are polymerases? -- 3.4. PCR Amplification of a Desired DNA Segment Of The Genome (Conventional Cloning) -- 3.5. Quickchange Site-Directed Mutagenesis -- Prelab Questions for Primer Design Lab -- Introduction to Primer Design Lab Report Outline and Point Distribution -- 4. Performing Site-Directed Mutagenesis -- 4.1. Learning Objective -- 4.2. Mini Project Flowchart -- 4.3. Review of Nucleic Acid Structure -- 4.4. How do Polymerases Work? -- 4.5. Polymerase Chain Reaction (PCR) in Practice -- 4.6. Why Did PCR Only Become Widely Available in the 1980s? -- 4.7. Applications of PCR -- Prelab Questions for Site-Directed Mutagenesis -- Site-directed Mutagenesis Lab Report Outline and Point Distribution -- PCR Worksheet. 
505 0 |a Note continued: 5. Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- 5.1. Learning Objective -- 5.2. Mini Project Flowchart -- 5.3. Purification of Plasmid DNA from Bacterial Cell (Plasmid Prep) -- 5.4. Transformation -- 5.5. Cell Growth -- 5.6. Purification of Plasmid DNA from Bacterial Cells -- 5.7. Agarose Gel Electrophoresis -- 5.8. Application of Agarose Gel Electrophoresis -- 5.9. DNA Quality Control Using UV Spectroscopy -- Prelab Questions for Plasmid Prep -- DNA Purification Lab Report Outline and Point Distribution -- Electrophoresis Problem Set -- 6. Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- 6.1. Learning Objective -- 6.2. Mini Project Flowchart -- 6.3. Synopsis -- 6.4. How do Restriction Endonucleases Work? -- 6.5. How do Restriction Enzymes Achieve Million-Fold Specificity? 
505 0 |a Note continued: 6.6. How Do We Judge Whether The Plasmid DNA is Successfully Linearized? -- 6.7. What are We Going to do in the Lab? -- Prelab Questions for DNA Linearization -- DNA Linearization Lab Report Outline and Point Distribution -- Worksheet -- Restriction Endonucleases -- Cloning Experiment Design -- Worksheet -- 7. Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- 7.1. Learning Objective -- 7.2. Mini Project Flowchart -- 7.3. How do RNA Polymerases Work? -- 7.4. How Does Transcription Start? -- 7.5. How Does Transcription End? -- 7.6. Transcription in Practice -- 7.7. What Are We Going To Do Today? -- Prelab Questions for RNA Transcription -- RNA Synthesis Lab Report Outline and Point Distribution -- 8. Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing Page and UV spectrometry -- 8.1. Learning Objective -- 8.2. Mini Project Flowchart -- 8.3. RNA Purification Methods -- 8.4. Denaturing Page -- 8.5. Phenol/chloroform Extraction. 
505 0 |a Note continued: 8.6. Column Purification -- Prelab Questions for RNA Purification -- RNA Purification Lab Report Outline and Point Distribution -- 9. Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- 9.1. Learning Objective -- 9.2. Mini Project Flowchart -- 9.3. What is Binding Affinity (KD)? -- 9.4. What is Fluorescence? -- 9.5. How Do We Measure Binding Affinity of the Tetracycline-Sensor RNA Complex? -- 9.6. How do We Evaluate Binding Affinity? -- 9.7. How do We Analyze Data? -- Analysis of Binding Experiments -- Binding Assays Prelab -- YkkCD sensor RNA -- Tetracycline Binding Lab Report Outline and Point Distribution -- 10. Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- 10.1. Learning Objectives -- 10.2. Biological Role of Serum Albumin -- 10.3. Fluoroquinoline Antibiotics -- 10.4. Protein Structure, Aromatic Amino Acids, and Fluorescence. 
505 0 |a Note continued: 10.5. Measuring Fluorescence -- 10.6. Synchronous Spectroscopy -- 10.7. Data Analysis -- Albumin -- Levofloxacin Binding Lab Report Outline and Point Distribution -- 11. Understanding the Importance of Buffers in Biological Systems -- 11.1. Learning Objectives -- 11.2. Introduction -- 11.3. Buffer Preparation -- Prelab for the Buffer Lab -- Buffer Lab Report Outline and Point Distributions -- Buffer Problem Set -- 12. Molecular Visualization of an Enzyme, Acetylcholinesterase -- 12.1. Learning Objectives -- 12.2. Introduction and Background -- 12.3. Introduction to Molecular Visualization Using the Program Chimera -- 12.4. Analysis of Acethylcholinesterase Using the Computer Visualization Program Chimera -- Molecular Visualization of Acethylcholinesterase Prelab -- Acetylcholinesterase Characteristics Worksheet -- 13. Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- 13.1. Learning Objective. 
505 0 |a Note continued: 13.2. Measuring the Catalytic Efficiency of Acetylcholinesterase -- 13.3. Running a Steady-State Enzyme Kinetics Experiment -- 13.4. Designing a Steady-State Experiment -- Prelab for AchE Kinetics -- Lab Report Outline and Point Distribution -- Enzyme Kinetics Worksheet -- 14. Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- 14.1. Learning Objective -- 14.2. Phosphatidylcholines -- 14.3. High Performance Liquid Chromatography (HPLC) -- 14.4. Quantifying Chromatography -- HPLC of Lipids Prelab -- HPLC of Phosphatidylcholines Lab Report Outline and Point Distribution -- HPLC Problem Set. 
590 |a Knovel  |b Knovel (All titles) 
650 0 |a Biochemistry  |v Laboratory manuals. 
650 0 |a Molecular biology  |v Laboratory manuals. 
650 0 |a Drug resistance in microorganisms  |v Laboratory manuals. 
655 7 |a elektronické knihy  |7 fd186907  |2 czenas 
655 9 |a electronic books  |2 eczenas 
700 1 |a Pattison, Scott,  |d 1947-  |e author. 
700 1 |a Rulka, Anna,  |e editor. 
776 0 8 |i Print version:  |a Fernandez, Timea.  |t Biochemistry laboratory manual for undergraduates : an inquiry-based approach.  |d Warsaw, [Poland] ; Berlin, [Germany] : De Gruyter Open, ©2014  |h vii, 174 pages  |z 9783110411324 
856 4 0 |u https://proxy.k.utb.cz/login?url=https://app.knovel.com/hotlink/toc/id:kpBLMUAIBI/biochemistry-laboratory-manual?kpromoter=marc  |y Full text 

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