Essentials of nucleic acid analysis : a robust approach

Over the last decade there has been a rapid development of molecular techniques, with an increasing range of instrumentation now available. The development of accompanying reference literature has not kept pace with technological advances and this poses significant challenges to the analyst. Essenti...

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Bibliographic Details
Other Authors Keer, Jacquie T., Birch, Lyndsey
Format Electronic eBook
LanguageEnglish
Published Cambridge, UK : Royal Society of Chemistry, ©2008.
Subjects
Nucleic acids > Analysis.
elektronické knihy
electronic books
Online AccessFull text
ISBN9781615833597
1615833595
9781847558213
1847558216
9780854043675
0854043675
Physical Description1 online resource (xxii, 248 pages) : illustrations (some color)

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Table of Contents:
  • Valid analytical molecular biology : the challenge
  • Quality in the analytical molecular biology laboratory
  • An introduction to method validation
  • DNA extraction
  • DNA quantification
  • PCR : factors affecting reliability and validity
  • Quantitative real-time PCR analysis
  • Multiplex PCR and whole genome amplification
  • Procedures for quality control of RNA samples for use in quantitative reverse transcription PCR
  • Microarrays.

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