http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
biomicrofabrication and biomicrofluidics
1 online resource (xiv, 352 pages) : illustrations (some color)
Written and edited by recognized experts in the field, the new Artech House Methods in Bioengineering series offers detailed guidance on authoritative methods for addressing specific bioengineering challenges. Offering a highly practical presentation of each topic, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. This unique volume presents leading-edge microfluidics methods used to handle, manipulate, and analyze cells, particles biological components (e.g., proteins and DNA) for microdiagnostics.
Methods in Bioengineering: Biomicrofabrication and Biomicrofluidics; Contents; Preface; Chapter 1 Microfabrication Techniques for Microfluidic Devices; 1.1 Introduction to microsystems and microfluidic devices; 1.2 Microfluidic systems: fabrication techniques; 1.3 Transfer processes; 1.3.1 Photolithography; 1.3.2 Molding; 1.4 Additive processes; 1.4.1 Growth of SiO2; 1.4.2 Deposition techniques; 1.5 Subtractive techniques; 1.5.1 Etching; 1.5.2 Chemical-mechanical polishing and planarization; 1.6 Bonding processes; 1.6.1 Lamination; 1.6.2 Wafer bonding methods; 1.7 Sacrificial layer techniques
1.8 Packaging processes1.8.1 Dicing; 1.8.2 Electrical interconnection and wire bonding; 1.8.3 Fluidic interconnection in microfluidic systems; 1.9 Materials for microfluidic and bio-MEMS applications; 1.9.1 Glass, pyrex, and quartz; 1.9.2 Silicon; 1.9.3 Elastomers; 1.9.4 Polydimethylsiloxane; 1.9.5 Epoxy; 1.9.6 SU-8 thick resists; 1.9.7 Thick positive resists; 1.9.8 Benzocyclobutene; 1.9.9 Polyimides; 1.9.10 Polycarbonate; 1.9.11 Polytetrafluoroethylene; 1.10 Troubleshooting table; 1.11 Summary; References; Chapter 2 Micropumping and Microvalving; 2.1 Introduction
2.2 Actuators for micropumps and microvalves2.2.1 Pneumatic actuators; 2.2.2 Thermopneumatic actuators; 2.2.3 Solid-expansion actuators; 2.2.4 Bimetallic actuators; 2.2.5 Shape-memory alloy actuators; 2.2.6 Piezoelectric actuators; 2.2.7 Electrostatic actuators; 2.2.8 Electromagnetic actuators; 2.2.9 Electrochemical actuators; 2.2.10 Chemical actuators; 2.2.11 Capillary-force actuators; 2.3 Micropumps; 2.3.1 Mechanical pump; 2.3.2 Nonmechanical pump; 2.4 Microvalves; 2.4.1 Mechanical valve; 2.4.2 Nonmechanical valve; 2.5 Outlook; 2.6 Troubleshooting; 2.7 Summary points; References
Chapter 3 Micromixing Within Microfluidic Devices3.1 Introduction; 3.2 Materials; 3.2.1 Microfluidic mixing devices; 3.2.2 Microfluidic interconnects; 3.2.3 Optical assembly; 3.2.4 Required reagents; 3.3 Experimental design and methods; 3.3.1 Passive micromixers; 3.3.2 Active micromixers; 3.3.3 Multiphase mixers; 3.4 Data acquisition, anticipated results, and interpretation; 3.4.1 Computer acquisition; 3.4.2 Performance metrics, extent of mixing, reaction monitoring; 3.5 Discussion and commentary; 3.6 Troubleshooting; 3.7 Application notes; 3.8 Summary points; References
Chapter 4 On-Chip Electrophoresis and Isoelectric Focusing Methods for Quantitative Biology4.1 Introduction; 4.1.1 Microfluidic electrophoresis supports quantitative biology and medicine; 4.1.2 Biomedical applications of on-chip electrophoresis; 4.2 Materials; 4.2.1 Reagents; 4.2.2 Facilities/equipment; 4.3 Methods; 4.3.1 On chip polyacrylamide gel electrophoresis (PAGE); 4.3.2 Polyacrylamide gel electrophoresis based isoelectric focusing; 4.3.3 Data acquisition, anticipated results, and interpretation; 4.3.4 Results and discussion; 4.4 Discussion of pitfalls; 4.5 Summary notes
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Includes bibliographical references and index.
Artech House methods in bioengineering series