Multi-parametric live cell microscopy of 3D tissue models
This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence...
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| Other Authors | |
|---|---|
| Format | Electronic eBook |
| Language | English |
| Published |
Cham, Switzerland :
Springer,
2017.
|
| Series | Advances in experimental medicine and biology ;
v. 1035. |
| Subjects | |
| Online Access | Full text |
| ISBN | 9783319673585 9783319673578 |
| ISSN | 0065-2598 ; |
| Physical Description | 1 online resource : illustrations (some color) |
Cover
Table of Contents:
- Part I. Introduction: 3D tissue models, methodology and toolkit. Current state-of-the-art 3D tissue models and their compatibility with live cell imaging / Katie Bardsley, Anthony J. Deegan, Alicia El Haj, and Ying Yang
- Simultaneous phosphorescence and fluorescence lifetime imaging by multi-dimensional TCSPC and multi-pulse excitation / Wolfgang Becker, Vladislav Shcheslavskiy, and Angelika Rück
- Quantitative live cell FLIM imaging in three dimensions / Alix Le Marois and Klaus Suhling
- Three-dimensional tissue models and available probes for multi-parametric live cell microscopy: a brief overview / Neil O'Donnell and Ruslan I. Dmitriev
- Part II. Manufacturing. Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues / Anastasia Shpichka, Anastasia Koroleva, Daria Kuznetsova, Ruslan I. Dmitriev, and Peter Timashev
- Part III. Application methods and protocols. Multi-parametric imaging of hypoxia and cell cycle in intestinal organoid culture / Irina A. Okkelman, Tara Foley, Dmitri B. Papkovsky, and Ruslan I. Dmitriev
- Imaging of intracellular pH in tumor spheroids using genetically encoded sensor SypHer2 / Elena V. Zagaynova, Irina N. Druzhkova, Natalia M. Mishina, Nadezhda I. Ignatova, Varvara V. Dudenkova, and Marina V. Shirmanova
- Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell / Takakazu Nakabayashi, Kamlesh Awasthi, and Nobuhiro Ohta
- Quantitative Imaging of Ca2+ by 3D-FLIM in live tissues / Asylkhan Rakymzhan, Helena Radbruch, and Raluca A. Niesner
- Live cell imaging of viscosity in 3D tumour cell models / Marina V. Shirmanova, Lubovʹ E. Shimolina, Maria M. Lukina, Elena V. Zagaynova, and Marina K. Kuimova
- Live imaging of cell invasion using a multicellular spheroid model and light-sheet microscopy / Marco Marcello, Rosalie Richards, David Mason, and Violaine Sée
- Raman imaging microscopy for quantitative analysis of biological samples / Shinji Kajimoto, Mizuki Takeuchi, and Takakazu Nakabayashi.