Future directions in metalloprotein and metalloenzyme research
This book covers the latest developments in metalloenzymes, including characterizing metal bridging in proteins and peptides, copper(II) complexes of marine peptides, high-spin Co(II) in model and metalloprotein systems to enzymes such as the molybdenum-containing enzymes, CW and pulse EPR of cytoch...
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| Other Authors | , |
|---|---|
| Format | Electronic eBook |
| Language | English |
| Published |
Cham :
Springer,
2017.
|
| Series | Biological magnetic resonance ;
v. 33. |
| Subjects | |
| Online Access | Full text |
| ISBN | 9783319591001 9783319590981 |
| ISSN | 0192-6020 ; |
| Physical Description | 1 online resource |
Cover
Table of Contents:
- Dedication; Preface; Contents; An Isotopic Dilution Strategy for Characterisation of Paramagnetic Metal Bridging of Proteins and Peptides; Introduction; Isotopic Labelling to Determine the First Coordination Sphere; Isotopic Dilution to Identify Metal-Bridged Peptide Oligomers; Methods; Experimental Preparation; Numerical Simulation; Examples; References; Structures, Electronics and Reactivity of Copper(II) Complexes of the Cyclic Pseudo-ƯPeptides of the Ascidians; Background; Prochloron: A Special Cyanobacterium; Ribosomal Expression of Cyclic Peptides.
- Metal Complexes of Cyclic Hexa- and Octapeptides Model Peptides; CuII and ZnII Coordination Chemistry of Westiellamide and Patellamide Derivatives: Solution Equilibria and Structures; In vitro Reactivity of Dinuclear Patellamide Complexes; Preliminary In vivo Studies of Patellamide Complexes; Conclusion and Outlook; References; Paramagnetic Resonance of High-Spin Co(II) in Biologically-Relevant Environments: Models to Metalloproteins; Introduction; Electronic Structure of High-Spin Co(II); Translation to EPR Properties and ZFS in High-Spin Co(II); Examples.
- Four- and Five-Coordinate Pyrazolylborates Parallel Mode EPR of Mononuclear Co(II) Complexes; ENDOR of Six-Coordinate Trigonal Co(II); Prospects for Pulsed EPR; Summary; References; EPR Spectroscopy on Mononuclear Molybdenum-Containing Enzymes; Introduction; An Overview on Molybdenum-Containing Enzymes; EPR Studies of Molybdoenzymes; Xanthine Oxidase Family; The Enzymes; EPR Studies of Mammalian Xanthine Oxidase; The "Very Rapid" Signal; The "Rapid" Signals; The "Slow" Signal; Signals Obtained From Inhibited and Inactive Forms of Xanthine Oxidase.
- Magnetic Interactions Within Xanthine Oxidase EPR Studies of Desulfovibrio Aldehyde Oxidoreductase; EPR Studies of Nicotinate Dehydrogenase and Carbon Monoxide Dehydrogenase; Sulfite Oxidase Family; The Enzymes; EPR Studies of Vertebrate Sulfite Oxidase; The "Low pH" Signal; The "High pH" Signal; The Family of "Oxo-Anion" Signals; Dimethylsulfoxide Reductase Family; The Enzymes; EPR Studies of Formate Dehydrogenases; EPR Studies of Nitrate Reductases; Periplasmatic Nitrate Reductases Signals; Assimilatory Nitrate Reductases Signals; Respiratory Nitrate Reductases Signals.
- Concluding RemarksReferences; CW and Pulse EPR of Cytochrome P450 to Determine Structure and Function; Introduction; Basic EPR Theory for Cytochromes P450; Interpretation of EPR Data Aided by Quantum Chemical Calculations; CW EPR to Characterise the Resting State and Substrate Bound P450 Active Site; Extracting Spin-State from g-Values and Its Relation to Optical and MCD Spectroscopy; The g-Values as an Indicator for Substrate Interactions; High-Resolution Pulsed EPR to Characterise the Resting State and Substrate Bound P450 Active Site.