Molecular diagnostics. Part 2, Clinical, veterinary, agrobotanical and food safety applications /

• Main Authors: Pelt-Verkuil, Elizabeth van, 1951- (Author), Leeuwen, W. B. van (Author), Witt, R. te (Author)
• Format: Electronic eBook
• Language: English
• Published: Singapore : Springer, 2017.
• Subjects: Molecular diagnosis.; elektronické knihy; electronic books
• ISBN: 9789811045110; 9789811045103
• Physical Description: 1 online resource (vii, 237 pages) : illustrations (some color)

This two volume book set provides a comprehensive and practical overview of the state-of-the-art molecular biological diagnostic strategies that are currently used in a wide variety of disciplines. The volumes cover: Clinical microbiology and virology Clinical chemistry Pathology Veterinary medicine...

Table of Contents:

• Introduction; 1 Molecular Diagnostics of Bacterial Pathogens; Abstract; 1.1 Introduction; 1.2 Molecular Diagnostics of Bacterial Sexually Transmitted Infections; 1.2.1 Molecular Diagnostics of Chlamydia trachomatis and Neisseria gonorrhoeae; 1.2.2 Molecular Diagnostics of Other Sexually Transmitted Bacteria; 1.3 Molecular Diagnostics of Bacterial Gastroenteritis; 1.3.1 Major Bacterial Pathogens for Routine Microbiological Diagnostics; 1.3.2 Other Bacterial Pathogens; 1.3.3 Molecular Diagnostics Algorithms for the Identification of Enteric Pathogens; 1.4 Bacterial Typing.
• 1.4.1 Typing Methods1.4.2 Pulsed-Field Gel Electrophoresis (PFGE); 1.4.3 Amplified Fragment Length Polymorphism Typing (AFLP); 1.4.4 Variable Number of Tandem Repeat (VNTR) Typing; 1.4.5 Random Amplification of Polymorphic DNA (RAPD); 1.4.6 Other Amplification Based Methods; 1.4.7 Single Locus Sequence Typing; 1.4.8 Spa Typing; 1.4.9 Multilocus Sequence Typing; 1.4.10 Optical Mapping; 1.4.11 Whole Genome Sequencing (WGS); 1.4.12 Typing for Action; 1.5 Conclusion; Recommended Literature; 2 Molecular Diagnostics of Parasitic Infections; Abstract; 2.1 Introduction; 2.2 DNA Isolation.
• 2.3 Intestinal Parasites2.3.1 Protozoa; 2.3.2 Helminths (Worms); 2.3.3 Algorithm for Faecal Examination; 2.4 Blood and Tissue Parasites; 2.4.1 Protozoa; 2.4.2 Helminths; 2.5 Concluding Remarks; References and Recommended Literature; 3 Virology; Abstract; 3.1 Introduction; 3.2 Isolation of Viral Nucleic Acids from Clinical Samples; 3.3 Molecular Diagnostics of Viruses; 3.3.1 Viral Detection Methods; 3.3.2 Primer/Probe Design; 3.3.2.1 Mismatch-Tolerance in Primers; 3.3.2.2 Probe Design; 3.4 Molecular Techniques for the Detection of Viral Variation.
• 3.5 Clinical Applications of Molecular Diagnostics3.5.1 Molecular Detection of Viral Infections; 3.5.2 Quantitative Detection of Viruses-Viral Load; 3.5.3 Genotyping and Genotypic Determination of Viral Resistance; 3.5.3.1 HIV-1; 3.5.3.2 Hepatitis B; 3.5.3.3 Hepatitis C; 3.5.3.4 Herpes Viruses; 3.5.3.5 Influenza Virus; 3.6 Conclusions; Recommended Literature; 4 Mycology; Abstract; 4.1 Introduction; 4.2 Molecular detection of invasive fungal infections; 4.2.1 Invasive Fungal Infections; 4.2.1.1 Diagnosis of Invasive Fungal Infection; 4.2.1.2 Conventional Species Identification.
• 4.2.1.3 Molecular Detection Methods4.2.1.4 Choice of Specimen; 4.2.1.5 Isolation of Fungal DNA; 4.2.2 Invasive Candidiasis; 4.2.2.1 Molecular Detection of Invasive Candidiasis; 4.2.3 Invasive Aspergillosis; 4.2.3.1 Molecular Detection; 4.2.4 Mucorales, Scedosporium, Fusarium and Other Opportunistic Filamentous Moulds; 4.2.4.1 Molecular Detection; 4.2.5 Pneumocystis jiroveci Pneumonia; 4.2.5.1 Molecular Identification; 4.3 Molecular diagnostics of dermatophyte infections; 4.3.1 Introduction; 4.3.2 Dermatophytosis; 4.3.3 Taxonomy and Epidemiology of Dermatophytes.