http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
from biophysical aspects to systems biology
This book focuses on the biophysical aspects of tip growth: How do physical parameters like pressure, water potential, electrical fields, or ion currents contribute to and influence this specialized and highly dynamic form of cell growth? It provides an updated and balanced overview of the current state of knowledge and future research perspectives regarding how pollen tubes' growth is driven and regulated by molecular interactions underlying the cellular processes. The individual chapters address topics ranging from molecular biophysical concepts to comprehensive omic studies and computational modeling of the tip growth process. In addition, a chapter on root hair cells is included to provide an alternative view on the underlying molecular principles of tip growth in general. Each chapter provides a summary of cutting-edge techniques, results and experimental data; a statement, hypothesis or conclusion on the importance of the aspect described for tip growth, particularly pollen tip growth; and addresses open gaps in our understanding and potential approaches to remedying them. The book offers experimental and theoretical solutions to help established researchers and newcomers to the field alike solve the many mysterious behaviors of pollen tube tips.
Editors' Biographies; Part I Introduction; 1 Pollen Tubes and Tip Growth: of Biophysics and Tipomics; 1.1 Introduction; 1.2 Some Unsolved (Bio)physical Aspects of Tip Growth; 1.3 Technical Improvements and a Wish List for the Future; 1.4 Tipomics: Pollen Systems Biology and Modelling Tip Growth; 1.5 Conclusion, Open Questions and Perspective; References; Part II Biophysics of Tip Growth; 2 Water Transport in Pollen; Abbreviations; 2.1 Introduction; 2.2 Water Potential, Water Transport and Pollen Tube Growth; 2.3 Measuring Water Potential Parameters and Water Transport in Pollen.
2.3.1 Protoplast Swell Assays2.3.2 Pressure Probe Technique; 2.4 Pollen Water Transport; 2.4.1 Aquaporins in Pollen; 2.4.2 Pollen Grain Rehydration; 2.4.3 Pollen Tube Elongation; 2.5 Osmosensing and Osmoregulation in Pollen; 2.6 Challenges for the Future: Reproduction and Drought Stress; 2.7 Conclusion and Perspective; References; 3 The Cytoskeleton of Pollen Tubes and How It Determines the Physico-mechanical Properties of Cell Wall; Abbreviations; 3.1 Introduction; 3.2 The Cytoskeleton of Pollen Tubes; 3.2.1 Actin Filaments.
3.2.2 Actin-Binding Proteins and Actin-Based Motors: How Do They Affect Cell Wall Synthesis?3.2.3 Microtubules; 3.2.4 Microtubule-Associated Proteins that May Take Part in Cell Wall Deposition; 3.3 Synthesis and Deposition of the Cell Wall in Pollen Tubes; 3.3.1 Secretion of Cell Wall Components: Pectins, Arabinogalactan Proteins, and the Cytoskeleton; 3.3.2 Plasma Membrane-Localized Synthesis of Cell Wall Polysaccharides; 3.3.3 Cytoskeleton-Based Delivery of Glucan Synthases; 3.4 Regulation of Cell Wall Synthesis Via Sucrose-Metabolizing Enzymes.
3.5 How the Cytoskeleton Affects the Physicochemical Properties of the Cell Wall3.6 Conclusion and Perspective; References; Part III Technical Improvements to Study Tip Growth; 4 Measuring Cytomechanical Forces on Growing Pollen Tubes; Abbreviations; 4.1 Introduction; 4.2 How Are Cytomechanical Parameters Measured?; 4.2.1 Measuring Turgor; 4.2.2 Measuring Penetration Forces; 4.2.3 Stiffness and Topography Mapping-Indentation Methods; 4.3 Cellular Force Microscopy; 4.3.1 General Considerations; 4.3.2 CFM Measurements on Pollen Tubes; 4.3.3 Limitations of the CFM Approach; 4.4 Improvements.
4.4.1 Microchannel Guidance4.4.2 Real-Time Computer Vision; 4.4.3 Dual-Axis Force Sensors; 4.5 Conclusions and Further Developments; References; 5 Microfluidic- and Microelectromechanical System (MEMS)-Based Platforms for Experimental Analysis of Pollen Tube Growth Behavior and Quantification of Cell Mechanical Properties; Abbreviations; 5.1 Introduction; 5.2 Design Principles and Considerations; 5.2.1 Basic Design and Fabrication; 5.2.2 Dimensions and Features of the Microfluidic Network; 5.2.3 Influence of Microchannel Geometry and Fluid Flow on Pollen Tube Performance.
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Includes bibliographical references and index.