Applications of Aminoacylation Ribozymes That Recognize the 3'-end of tRNA

In this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) these base...

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Bibliographic Details
Main Author: Terasaka, Naohiro. (Author)
Corporate Author: SpringerLink (Online service)
Format: eBook
Language: English
Published: Tokyo : Springer Japan : Imprint: Springer, 2017.
Series: Springer Theses, Recognizing Outstanding Ph.D. Research,
Subjects:
Chemistry.
Biotechnology.
Bioorganic chemistry.
Catalysis.
Nucleic acids.
elektronické knihy
electronic books
ISBN: 9784431565178
Physical Description: 1 online resource (XII, 89 p. 38 illus., 28 illus. in color.)

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008 170309s2017 ja | s |||| 0|eng d
020 |a 9784431565178 
024 7 |a 10.1007/978-4-431-56517-8  |2 doi 
100 1 |a Terasaka, Naohiro.  |e author. 
245 1 0 |a Applications of Aminoacylation Ribozymes That Recognize the 3'-end of tRNA /  |c by Naohiro Terasaka. 
264 1 |a Tokyo :  |b Springer Japan :  |b Imprint: Springer,  |c 2017. 
300 |a 1 online resource (XII, 89 p. 38 illus., 28 illus. in color.) 
336 |a text  |b txt  |2 rdacontent 
337 |a počítač  |b c  |2 rdamedia 
338 |a online zdroj  |b cr  |2 rdacarrier 
490 1 |a Springer Theses, Recognizing Outstanding Ph.D. Research,  |x 2190-5053 
505 0 |a General introduction -- Discovery of human microRNA precursor binding to folic acid by small RNA transcriptomic SELEX -- Orthogonal ribosome-tRNAs pair by engineering of peptidyl transferase center -- General conclusion. 
506 |a Plný text je dostupný pouze z IP adres počítačů Univerzity Tomáše Bati ve Zlíně nebo vzdáleným přístupem pro zaměstnance a studenty 
520 |a In this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) these base pairs can be substituted with other base pairs; and (iii) various activated amino acids can be used as substrates including both canonical and noncanonical amino acids. This flexible aminoacylation of RNAs by flexizymes was used to label endogenous tRNAs to be removed, and in vitro selection using the tRNA-depleted library enabled the discovery of the novel interaction between the microRNA precursor and metabolites. Flexizymes are also used to prepare various aminoacyl-tRNAs bearing mutations at the 3'-end to engineer the translation machinery and to develop the orthogonal translation machinery. The first part of the research demonstrated that SELEX is appropriate for discovering the interaction between small RNA and ligands, and suggested that more RNA motif binding to small molecules exists in small RNAs. The second part opened a door to new opportunities for in vitro synthetic biology involving the engineering of the genetic codes and translation machineries. This research also indicated the great potential of aminoacylation by flexizymes to be applied in various fields of RNA research, which is beneficial for RNA researchers. 
650 0 |a Chemistry. 
650 0 |a Biotechnology. 
650 0 |a Bioorganic chemistry. 
650 0 |a Catalysis. 
650 0 |a Nucleic acids. 
655 7 |a elektronické knihy  |7 fd186907  |2 czenas 
655 9 |a electronic books  |2 eczenas 
710 2 |a SpringerLink (Online service) 
776 0 8 |i Printed edition:  |z 9784431565154 
830 0 |a Springer Theses, Recognizing Outstanding Ph.D. Research,  |x 2190-5053 
856 4 0 |u https://proxy.k.utb.cz/login?url=http://dx.doi.org/10.1007/978-4-431-56517-8  |y Plný text 
992 |c NTK-SpringerCHEMS 
999 |c 100454  |d 100454 
993 |x NEPOSILAT  |y EIZ 

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