Polymerase chain reaction with lateral flow sensor assay for the identification of horse meat in raw and processed meat products.

Bibliographic Details
Title: Polymerase chain reaction with lateral flow sensor assay for the identification of horse meat in raw and processed meat products.
Authors: Chen Y; College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, Jilin, China., Wang Y; College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, Jilin, China., Xiao M; College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, Jilin, China., Wei S; College of Agronomy, Jilin Agricultural Science and Technology University, Jilin 132101, Jilin, China., Yang H; College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, Jilin, China., Yin R; College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, Jilin, China. Electronic address: 13364412403@163.com.
Source: Food chemistry [Food Chem] 2021 May 30; Vol. 345, pp. 128840. Date of Electronic Publication: 2020 Dec 11.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE
Imprint Name(s): Publication: Barking : Elsevier Applied Science Publishers
Original Publication: Barking, Eng., Applied Science Publishers.
MeSH Terms: DNA/*analysis , Horses/*genetics , Meat/*analysis , Meat Products/*analysis , Polymerase Chain Reaction/*methods, Animals ; DNA/metabolism ; DNA Probes/metabolism ; Food Analysis ; Reproducibility of Results
Abstract: A simple, rapid and accurate detection method for the authentication of animal species is urgently required in the food detection field. The present study established a horse-specific polymerase chain reaction integrated with a lateral flow sensor assay (Horse-PCR-LFS) for the rapid detection of horse meat. In this test, a cytb gene sequence of horse was amplified using PCR, the PCR amplicon was checked with the lateral flow sensor assay, and the result of the sensor can be read within 2-3 min by the naked eye. The detection limit of the test was up to 0.01% horse meat in artificially adulterated meat mixtures, the assay also successfully detected horse DNA in various commercial food samples. As a rapid and user-friendly molecular detection tool, this test provides an accurate detection format for the identification of horse and offers solutions to problems related to animal meat adulteration and animal-origin food safety and traceability.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Contributed Indexing: Keywords: Horse; Meat authentication; PCR; Sensor
Substance Nomenclature: 0 (DNA Probes)
9007-49-2 (DNA)
Entry Date(s): Date Created: 20210219 Date Completed: 20210325 Latest Revision: 20210325
Update Code: 20210914
DOI: 10.1016/j.foodchem.2020.128840
PMID: 33601658
Published: 2021 May 30
Database: MEDLINE

Academic Journal

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