Comparative genomics of Clostridium species associated with vacuum-packed meat spoilage.

Bibliographic Details
Title: Comparative genomics of Clostridium species associated with vacuum-packed meat spoilage.
Authors: Palevich N; AgResearch Limited, Grasslands Research Centre, Palmerston North, New Zealand. Electronic address: nik.palevich@agresearch.co.nz., Palevich FP; AgResearch Limited, Grasslands Research Centre, Palmerston North, New Zealand., Maclean PH; AgResearch Limited, Grasslands Research Centre, Palmerston North, New Zealand., Altermann E; AgResearch Limited, Grasslands Research Centre, Palmerston North, New Zealand; Riddet Institute, Massey University, Palmerston North, New Zealand., Gardner A; AgResearch Limited, Grasslands Research Centre, Palmerston North, New Zealand., Burgess S; Molecular Epidemiology and Veterinary Public Health Laboratory (mEpiLab), Infectious Disease Research Centre, School of Veterinary Science, Massey University, Palmerston North, New Zealand., Mills J; AgResearch Limited, Grasslands Research Centre, Palmerston North, New Zealand., Brightwell G; AgResearch Limited, Grasslands Research Centre, Palmerston North, New Zealand.
Source: Food microbiology [Food Microbiol] 2021 May; Vol. 95, pp. 103687. Date of Electronic Publication: 2020 Nov 21.
Publication Type: Comparative Study; Journal Article
Language: English
Journal Info: Publisher: Elsevier Country of Publication: England NLM ID: 8601127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9998 (Electronic) Linking ISSN: 07400020 NLM ISO Abbreviation: Food Microbiol Subsets: MEDLINE
Imprint Name(s): Publication: London : Elsevier
Original Publication: London ; Orlando : Academic Press, c1984-
MeSH Terms: Clostridium/*genetics , Clostridium/*isolation & purification , Meat Products/*microbiology, Animals ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Cattle ; Clostridium/classification ; Esterases/genetics ; Esterases/metabolism ; Food Packaging ; Food Safety ; Genome, Bacterial ; Glycoside Hydrolases/genetics ; Glycoside Hydrolases/metabolism ; Meat Products/analysis ; New Zealand ; Polysaccharide-Lyases/genetics ; Polysaccharide-Lyases/metabolism ; Vacuum
Abstract: Bacterial species belonging to the genus Clostridium have been recognized as causative agents of blown pack spoilage (BPS) in vacuum packed meat products. Whole-genome sequencing of six New Zealand psychrotolerant clostridia isolates derived from three meat production animal types and their environments was performed to examine their roles in BPS. Comparative genome analyses have provided insight into the genomic diversity and physiology of these bacteria and divides clostridia into two separate species clusters. BPS-associated clostridia encode a large and diverse spectrum of degradative carbohydrate-active enzymes (CAZymes) that enable them to utilize the intramuscular carbohydrate stores and facilitate sporulation. In total, 516 glycoside hydrolases (GHs), 93 carbohydrate esterases (CEs), 21 polysaccharide lyases (PLs), 434 glycosyl transferases (GTs) and 211 carbohydrate-binding protein modules (CBM) with predicted activities involved in the breakdown and transport of carbohydrates were identified. Clostridia genomes have different patterns of CAZyme families and vary greatly in the number of genes within each CAZy category, suggesting some level of functional redundancy. These results suggest that BPS-associated clostridia occupy similar environmental niches but apply different carbohydrate metabolism strategies to be able to co-exist and cause meat spoilage.
(Crown Copyright © 2020. Published by Elsevier Ltd. All rights reserved.)
Contributed Indexing: Keywords: Carbohydrate metabolism; Clostridia; Clostridium estertheticum; Clostridium tagluense; Comparative genomics; Genomics; Spoilage flora; Vacuum-packageded meat
Substance Nomenclature: 0 (Bacterial Proteins)
EC 3.1.- (Esterases)
EC 3.2.1.- (Glycoside Hydrolases)
EC 4.2.2.- (Polysaccharide-Lyases)
Entry Date(s): Date Created: 20210105 Date Completed: 20210603 Latest Revision: 20210603
Update Code: 20210623
DOI: 10.1016/j.fm.2020.103687
PMID: 33397617
Published: 2021 May
Database: MEDLINE

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