Screening of estrogenic endocrine-disrupting chemicals in meat products based on the detection of vitellogenin by enzyme-linked immunosorbent assay.

Bibliographic Details
Title: Screening of estrogenic endocrine-disrupting chemicals in meat products based on the detection of vitellogenin by enzyme-linked immunosorbent assay.
Authors: Li X; Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan, 250014, PR China., Liu X; Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan, 250014, PR China., Jia Z; Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan, 250014, PR China., Wang T; Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan, 250014, PR China., Zhang H; Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan, 250014, PR China. Electronic address: zhanghongyan@sdnu.edu.cn.
Source: Chemosphere [Chemosphere] 2021 Jan; Vol. 263, pp. 128251. Date of Electronic Publication: 2020 Sep 04.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 0320657 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1298 (Electronic) Linking ISSN: 00456535 NLM ISO Abbreviation: Chemosphere Subsets: MEDLINE
Imprint Name(s): Publication: Oxford : Elsevier Science Ltd
Original Publication: Oxford, New York, : Pergamon Press.
MeSH Terms: Endocrine Disruptors*/toxicity , Meat Products*, Animals ; Enzyme-Linked Immunosorbent Assay ; Estrogens/toxicity ; Estrone ; Female ; Male ; Vitellogenins
Abstract: Vitellogenin (VTG) is typically produced by females but when present in males can indicate contamination with estrogenic endocrine-disrupting chemicals (EDCs). Here we used primary hepatocytes isolated from male crucian carp uncontaminated with estrogenic EDCs as a culture model. Nine EDCs were used for validation, and an enzyme-linked immunosorbent assay (ELISA) was used to quantify VTG production by primary hepatocytes. The lower limit of detection of 17β-E2, DES, and HES using the method was 10 -12  M. Fresh pork was roasted, and non-hydrolytic acetonitrile-vortexing was used to extract potential estrogenic EDCs. The extracted substances were separated by thin-layer chromatography (TLC), and dimethyl terephthalate present in roast pork was found to induce high VTG concentrations. Therefore, the detection of VTG by ELISA provides a sensitive and reliable method for detecting known and unknown estrogenic EDCs in food products.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Contributed Indexing: Keywords: Dimethyl terephthalate; Enzyme-linked immunosorbent assay; Estrogenic endocrine-disrupting chemicals; Meat products; Vitellogenin
Substance Nomenclature: 0 (Endocrine Disruptors)
0 (Estrogens)
0 (Vitellogenins)
2DI9HA706A (Estrone)
Entry Date(s): Date Created: 20201210 Date Completed: 20201221 Latest Revision: 20201221
Update Code: 20210914
DOI: 10.1016/j.chemosphere.2020.128251
PMID: 33297196
Published: 2021 Jan
Database: MEDLINE

Academic Journal

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