Lateral flow immunoassay for sensitive detection of undeclared chicken meat in meat products.

Bibliographic Details
Title: Lateral flow immunoassay for sensitive detection of undeclared chicken meat in meat products.
Authors: Hendrickson OD; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia., Zvereva EA; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia., Vostrikova NL; V.M. Gorbatov Federal Research Center for Food Systems of the Russian Academy of Sciences, Talalikhina Street 26, Mocow 109316, Russia., Chernukha IM; V.M. Gorbatov Federal Research Center for Food Systems of the Russian Academy of Sciences, Talalikhina Street 26, Mocow 109316, Russia., Dzantiev BB; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia., Zherdev AV; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia. Electronic address: zherdev@inbi.ras.ru.
Source: Food chemistry [Food Chem] 2021 May 15; Vol. 344, pp. 128598. Date of Electronic Publication: 2020 Nov 11.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE
Imprint Name(s): Publication: Barking : Elsevier Applied Science Publishers
Original Publication: Barking, Eng., Applied Science Publishers.
MeSH Terms: Immunoassay/*methods , Meat/*analysis , Meat Products/*analysis, Animals ; Cattle ; Chickens ; Cooking ; Gold/chemistry ; Immunoglobulins/analysis ; Limit of Detection ; Metal Nanoparticles/chemistry ; Swine
Abstract: This study presents the development of an immunochromatographic test system aimed at the detection of chicken additives in meat products. It is based on sandwich-format lateral flow immunoassay (LFIA) of immunoglobulins as a biomarker for species identification. The LFIA based on gold nanoparticles as a label for anti-species antibodies was used to determine chicken immunoglobulins and, accordingly, chicken meat in food products. Absence of cross-reactivity with mammalian species tested in the study confirmed high specificity of the determination. The test system showed good sensitivity and rapidity, allowing for the detection of as low as 0.063% (w/w) chicken meat in raw meat mixtures within 20 min. As a result of the testing of raw and cooked meat products, it was shown that the test system can reliably recognize specific immunoglobulins even after heat processing. The proposed assay can be recommended for rapid on-site screening control of the composition and quality of meat products.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Contributed Indexing: Keywords: Chicken additives; Immunoglobulins; Lateral flow immunoassay; Meat adulteration
Substance Nomenclature: 0 (Immunoglobulins)
7440-57-5 (Gold)
Entry Date(s): Date Created: 20201124 Date Completed: 20210201 Latest Revision: 20210201
Update Code: 20210914
DOI: 10.1016/j.foodchem.2020.128598
PMID: 33229162
Published: 2021 May 15
Database: MEDLINE

Academic Journal

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