Phenotypic and genotypic characterization of biofilm forming, antimicrobial resistant, pathogenic Escherichia coli isolated from Indian dairy and meat products.

Bibliographic Details
Title: Phenotypic and genotypic characterization of biofilm forming, antimicrobial resistant, pathogenic Escherichia coli isolated from Indian dairy and meat products.
Authors: Bhardwaj DK; Department of Basic and Applied Sciences, NIFTEM, Sonipat 131028, Haryana, India., Taneja NK; Department of Basic and Applied Sciences, NIFTEM, Sonipat 131028, Haryana, India. Electronic address: neetu.taneja@niftem.ac.in., Dp S; Department of Basic and Applied Sciences, NIFTEM, Sonipat 131028, Haryana, India., Chakotiya A; Department of Basic and Applied Sciences, NIFTEM, Sonipat 131028, Haryana, India., Patel P; Department of Basic and Applied Sciences, NIFTEM, Sonipat 131028, Haryana, India., Taneja P; Department of Life Sciences, Sharda University, Greater Noida, Uttar Pradesh, India., Sachdev D; Department of Basic and Applied Sciences, NIFTEM, Sonipat 131028, Haryana, India., Gupta S; Institute of Liver and Biliary Sciences, Vasant Kunj, New Delhi, India., Sanal MG; Institute of Liver and Biliary Sciences, Vasant Kunj, New Delhi, India.
Source: International journal of food microbiology [Int J Food Microbiol] 2021 Jan 02; Vol. 336, pp. 108899. Date of Electronic Publication: 2020 Sep 30.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Elsevier Science Publishers Country of Publication: Netherlands NLM ID: 8412849 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3460 (Electronic) Linking ISSN: 01681605 NLM ISO Abbreviation: Int J Food Microbiol Subsets: MEDLINE
Imprint Name(s): Original Publication: Amsterdam : Elsevier Science Publishers, c1984-
MeSH Terms: Anti-Bacterial Agents/*pharmacology , Dairy Products/*microbiology , Escherichia coli/*drug effects , Escherichia coli/*genetics , Meat Products/*microbiology, Animals ; Biofilms ; Escherichia coli/pathogenicity ; Food Safety ; Foodborne Diseases/microbiology ; Genotype ; Humans ; India ; Phenotype ; Quorum Sensing ; Virulence/genetics
Abstract: Escherichia coli are commensal gastrointestinal microflora of humans, but few strains may cause food-borne diseases. Present study aimed to identify antimicrobial resistant (AMR), biofilm-forming E. coli from Indian dairy and meat products. A total of 32 E. coli isolates were identified and evaluated for biofilm-formation. EMC17, an E. coli isolate was established as a powerful biofilm-former that attained maximum biofilm-formation within 96 h on glass and stainless-steel surfaces. Presence and expression of virulence-associated genes (adhesins, invasins and polysaccharides) and ability to adhere and invade human liver carcinoma HepG2 cell lines implicates EMC17 to be pathotype belonging to Extra-intestinal Pathogenic E. coli (ExPEC). Antibiotic profiling of EMC17 identified it as multi-drug resistant (MDR) strain, possessing extended spectrum β-lactamases (ESBL's) and biofilm phenotype. Early production of quorum sensing molecules (AHLs) alongside EPS production facilitated early onset of biofilm formation by EMC17. Furthermore, the biofilm-forming genes of EMC17 were significantly upregulated 3-27 folds in the biofilm-state. This study showed prevalence of MDR, biofilm-forming, pathogenic E. coli in Indian dairy and meat products that potentially serve as reservoirs for transmission of antimicrobial-resistant (AMR) genes of bacteria from food to humans and pose serious food safety threat.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Contributed Indexing: Keywords: Antimicrobial resistance (AMR); Biofilms; E. coli; Food safety
Substance Nomenclature: 0 (Anti-Bacterial Agents)
Entry Date(s): Date Created: 20201107 Date Completed: 20201204 Latest Revision: 20201214
Update Code: 20210623
DOI: 10.1016/j.ijfoodmicro.2020.108899
PMID: 33160121
Published: 2021 Jan 02
Database: MEDLINE

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