Virulence genotyping and antimicrobial resistance profiles of Yersinia enterocolitica isolated from meat and meat products in Egypt.

Bibliographic Details
Title: Virulence genotyping and antimicrobial resistance profiles of Yersinia enterocolitica isolated from meat and meat products in Egypt.
Authors: Younis GA; Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, 35516, Mansoura, Egypt., Elkenany RM; Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, 35516, Mansoura, Egypt., Dowidar HA; Al-Azhar University, Mansoura, Egypt.
Source: Brazilian journal of biology = Revista brasleira de biologia [Braz J Biol] 2021 Mar-May; Vol. 81 (2), pp. 424-436.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: International Institute of Ecology Country of Publication: Brazil NLM ID: 101129542 Publication Model: Print Cited Medium: Internet ISSN: 1678-4375 (Electronic) Linking ISSN: 15196984 NLM ISO Abbreviation: Braz J Biol Subsets: MEDLINE
Imprint Name(s): Original Publication: São Carlos, SP [Brazil] : International Institute of Ecology, [2000]-
MeSH Terms: Meat Products* , Yersinia enterocolitica*/genetics, Anti-Bacterial Agents/pharmacology ; Drug Resistance, Bacterial/genetics ; Egypt ; Genotype ; Humans ; Meat ; Phylogeny ; RNA, Ribosomal, 16S ; Virulence/genetics
Abstract: Pathogenic Yersinia enterocolitica (Y. enterocolitica) is one of the food-borne entero-pathogen responsible for yersiniosis in humans. The purpose of this research was to survey the prevalence, virulence-associated genes, and antimicrobial resistance of Y. enterocolitica isolated from meat and meat product samples in Egypt. Forty-one (5.9%) out of 700- samples of chicken meat, beef, ground beef, and sausage were positive Y. enterocolitica with a high prevalence in chicken meat (12%). Five virulence genes (ail, inv, ystA, ystB, and yadA) were characterized among 41 Y. enterocolitica isolates with variable frequencies. Among the strains tested, the ystB gene was detected with a high percentage (78.1%), followed by inv gene (70.7%), ail gene (14.6%), ystA gene (12.2%), and yadA gene (2.4%). A high resistance rate was estimated to amoxicillin-clavulanic acid (100%), followed by cefazolin (95%), ampicillin (65.9%), and doxycycline (51.2%), whilst a high sensitivity rate was observed to gentamicin and ciprofloxacin (97.6% each). Interestingly, the multidrug resistance was specified in the 70.7% of strains and showing 13 resistance patterns. Based on nucleotide sequence analysis of the 16s rRNA gene, the phylogenetic tree showed the genetic relatedness amongst Y. enterocolitica isolates. These findings highlighted the emergence of virulent and multidrug-resistant pathogenic Y. entrocolitica in retailed meat and meat products in Egypt.
Substance Nomenclature: 0 (Anti-Bacterial Agents)
0 (RNA, Ribosomal, 16S)
Entry Date(s): Date Created: 20200930 Date Completed: 20210222 Latest Revision: 20210222
Update Code: 20210623
DOI: 10.1590/1519-6984.229998
PMID: 32996992
Published: 2021 Mar-May
Database: MEDLINE

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